Author(s)

Samira M. Sallam¹, Abdelsattar M. Sallam², El-Sayed M. El-Sayed², L. I. Abo Salem¹, Mona M. Rizk¹

¹Department of Physics, Faculty of Science, Benha University, Benha, Egypt.
²Department of BioPhysics, Faculty of Science, Ain Shams University, Cairo, Egypt.

The aim of the present work is to investigate the effect of the He-Ne laser irradiation on the whole human blood (HB) in order to enhance the conditions of conservation. The HB was irradiated by He-Ne laser; (λ = 632 nm, continuous wave, power 30 mW, 2 mm diameter beam spot), electrical properties and complete blood count CBC were measured at three doses (0.0287, 0.0563 and 0.198 J/cm³) to the relevant best exposure dose during storage periods 9, 24, 30, 35 & 50 days. The irradiation process with the selected dose was performed by the exposure of the laser beam on the blood sample flow through narrow tube of cross section area, 0.0831 cm². Blood dielectric parameters, (electric conductivity, dielectric constant, dielectric loss and dipole moment) and CBC, (red blood cell, white blood cell, hematocrit, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin, and mean cell or corpuscular hemoglobin in concentration) were measured. The obtained results were compared with that of the control and showed that the best irradiation exposure dose suitable for increasing the time of blood storage with minimum changes in properties is 0.198 J/cm³ and storage period of about 50 days. The present study revealed that irradiation by He-Ne laser could be considered a good means to improve the conservation conditions of human blood.

He-Ne Laser, Whole Blood Storage, Electrical Properties, Dielectric Constant

Sallam, S. , Sallam, A. , El-Sayed, E. , Salem, L. and Rizk, M. (2015) Enhancement of Human Blood Storage Period by Irradiation of Low Level He-Ne Laser. Journal of Biophysical Chemistry, 6, 77-86. doi: 10.4236/jbpc.2015.63008.