Author(s)

Jinlong Ma, Guobin Jiang, Shanjing Yao, Hua Jin, Changhai Wang

Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China.
Environment and Resources College, Dalian Nationalities University, Dalian, China.
Life Science College, Dalian Nationalities University, Dalian, China.
Nanjing Agricultural University, Nanjing, China.

In the current study, in order to change the permeability of cell membrane and solve the problem of linked group of fungi mycelium, the method of adjusting osmotic pressure of medium and adding tween-80 was established. The utilized strain with relatively high exopolysaccharide (EPS) yield and low pigment level was obtained after the rejuvenation and sifting of long-preserved Aureobasidium pullulans strain. The optimal proportion of substrate was determined by means of orthogonal test. The transformation ratio of EPS was increased by 10% - 20% and the pigment content was greatly reduced. The fermenting liquor is between creamy white and pale yellow, and the white primary product can be gained without decolourization step. Furthermore, to magnify to 5 L bioreactor can get the similar result.

Aureobasidium pullulans; Exopolysaccharide (EPS); Orthogonal Test; Group of Fungi Mycelium