Identification of Molecular Markers Linked to Leaf Rust Resistance Genes in Wheat and Their Detection in the Local Near-Isogenic Line
Navjot Kaur Dhillon, Harcharan Singh Dhaliwal
.
DOI: 10.4236/ajps.2011.23049   PDF    HTML     5,337 Downloads   10,867 Views   Citations

Abstract

Sixty-five random amplified polymorphic DNA (RAPD) primers were used for the detection of polymorphism among recipient and donor parents and their isogenic lines linked to leaf rust resistance genes, Lr9 and the resistant gene in kharchia local mutant KLM4-3B. Three primers showed polymorphism among recurrent parent, donor parent and isogenic lines.

Share and Cite:

Dhillon, N. and Dhaliwal, H. (2011) Identification of Molecular Markers Linked to Leaf Rust Resistance Genes in Wheat and Their Detection in the Local Near-Isogenic Line. American Journal of Plant Sciences, 2, 433-437. doi: 10.4236/ajps.2011.23049.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] M. G. Eversmeyer and L. E. Browder, “Effect of Leaf and Stem Rust on 1973 Kansaswheat Yields,” Plant Disease Reporter, Vol. 58, No. 5, 1974, pp. 469-471.
[2] R. G. Saini and A K Gupta, “Genes for Resistance to Brown Rust Puccinia recondita) in Wheat. II. Lr Genes in Frontana, WG138 and E6360,” Cereal Research Communications, Vol. 7, 1979, pp. 289-291.
[3] D. Anand, R. G. Saini and A. K. Gupta, “Slow Leaf Rust Development Due to Combination of Some Genes in Wheat,” Plant Disease Reporter, Vol. 3, 1988, p. 97.
[4] M. Seck, A. P. Roelfs and P. S. Teng, “Effect of Leaf Rust Puccinia recondite triticii on Yield of Four Isogenic Wheat Lines,” Crop Production, Vol. 7, No. 1, 1988, pp. 39-43. doi:10.1016/0261-2194(88)90036-1
[5] K. V. Subha Rao, J. P. Snow and G. T. Berggren, “Effect or Growth Stage and Initial Inoculum Level on Leaf Rust Development and Yield Loss Caused by Puccinia recondita f. sp. tritici,” Journal of Phytopathology, Vol. 127, No. 3, 1989, pp. 200-210. doi:10.1111/j.1439-0434.1989.tb01130.x
[6] M. Baum, E. S. Laguadah and R. Appels, “Wide Crosses in Cereals,” Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 43, 1992, pp. 117-143. doi:10.1146/annurev.pp.43.060192.001001
[7] E. R. Sears, “The Transfer of Leaf Rust Resistance from Aegilops umbellulata into Wheat,” Brookhaven Symposia in Biology, Vol. 9, 1956, pp. 1-21.
[8] A. S. Soliman, E. Y. Heyne and C. O. Johnston, “Resistance to Leaf Rust in Wheat Derived from Chinese Aegilops umbellulata Translocation Lines,” Crop Science, Vol. 3, No. 3, 1963, pp. 254-256. doi:10.2135/cropsci1963.0011183X000300030025x
[9] S. L. Dellaporta, J. Wood and J. B. Hicks, “A Plant DNA Mini Preparation. Version 11,” Plant Molecular Biology Reporter, Vol. 1, No. 4, 1983, pp. 19-21. doi:10.1007/BF02712670
[10] J. G. K. Williams, A. R. Kubelik, K. J. Livak, J. A. Rafulski and S. V. Tingey,“ DNA Polymorphisms Amplified by Arbitrary Primers Are Useful Genetic Marker,” Nucleic Acids Research, Vol. 18, No. 22, 1990, pp. 6531-6535. doi:10.1093/nar/18.22.6531
[11] G. B. Martin, J. G. K. Williams and S. D. Tanksley, “Rapid Identification of Markers Linked to a Pseudomonas Resistance Gene in Tomato by Using Random Primers and Near Isogenic Lines,” Proceedings of the National Academy of Sciences of the USA, Vol. 88, No. 6, 1991, pp. 2236-2340. doi:10.1073/pnas.88.6.2336
[12] L. R. M. Klein, A. Vermunt, R. Weide, T. Liharska and R. Zabel, “Isolation of Molecular Markers for Tomato (L. esculentum) Using Random Amplified Polymorphic DNA (RAPD),” Theoretische und Angewandte Genetik, Vol. 83, No. 1, 1991, pp. 108-114.
[13] P. C. Ronald, B. Albano, R. Tabien, L. Abenes, K. S. Wu, S. McCouch and S. D. Tanskley, “Genetic and Physical Analysis of the Rice Bacterial Blight Disease Resistance Locus, Xa21,” Molecular & General Genetics, Vol. 236, No. 1, 1992, pp. 113-120.
[14] S. Yoshimura, A. Yoshimura, N. Iwata, S. R. McCouch, M. L. Abenes, M. R. Baraoiden, T. W. Mew and K. J. Nelson, “Tagging and Combining Bacterial Blight Resistance Genes in Rice Using RAPD and RFLP Markers,” Molecular Breeding, Vol. 1, No. 4, 1995, pp. 375-387.
[15] S. Yoshimura, A. Yoshimura, R. J. Nelson, T. W. Mew and N. Iwata, “Tagging Xa-l the Bacterial Blight Resistant Gene in Rice, by Using RAPD Markers,” Breed Science, Vol. 45, 1995, pp. 81-85.
[16] I. Dweikat, H. Ohm, S. MacKenzie, F. Patterson, S. Cambron and R. Rateliffe, “Association of a DNA Marker with Hessian Fly Resistance Gene H9 in Wheat,” Theoretical and Applied Genetics, Vol. 89, No. 7-8, 1994, pp. 964-968. doi:10.1007/BF00224525
[17] J. D. Procunier, T. F. Townley Smith, S. Fox, S. Prashar, M. Gray, W. K. Kim, Czarnecki and P. L. Dyck, “PCR-Based RAPD/DGGE Markers Linked to Leaf Rust Resistance Genes Lr29 and Lr25 in Wheat (T. aestivum),” Journal of Genetics and Breeding, Vol. 49, No. 1, 1995, pp. 87-91.
[18] G. Schachermayr, M. Messmer, C. Feuillet, H. Winzeler, M. Winzeler and B. Keller, “Identification of Molecular Markers Linked to the Agropyron elongatum-Derived Leaf Rust Resistance Gene Lr24 in Wheat,” Theoretical and Applied Genetics, Vol. 90, No. 7-8, 1995, pp. 982-990. doi:10.1007/BF00222911
[19] L. E. Talbert, P. L. Bruckner, L. Y. Smith, R. Sears and T. J. Martin, “Development of PCR Markers Linked to Resistance to Wheat Streak Mosaic Virus in Wheat,” Theoretical and Applied Genetics, Vol. 93, No. 3, 1996, pp. 463-467. doi:10.1007/BF00223191
[20] S. Chao, J. Sharp, A. J. Worland, E. J. Warham, R. Koebner and M. D. Gale, “RFLP-Based Genetic Maps of Wheat Homoeologous Group 7 Chromosomes,” Theoretical and Applied Genetics, Vol. 78, No. 4, 1998, pp. 495-504. doi:10.1007/BF00290833
[21] L. N. W. Kam-Morgan, B. S. Gill and S. Muthukrishan, “DNA Restriction Fragment Length Polymorphisms: A Strategy for Genetic Mapping of D-Genome of Wheat,” Genome, Vol. 32, No. 4, 1989, pp. 724-732.
[22] N. A. Dadkhodaie, H. Karaoglou, C. R. Wellings and R. F. Park, “Mapping Genes Lr53 and Yr35 on the Short Arm of Chromosome 6B of Common Wheat with Microsatellite Markers and Studies of Their Association with Lr36,” Theoretical and Applied Genetics, Vol. 122, No. 3, 2010, pp. 479-487. doi:10.1007/s00122-010-1462-y
[23] G. F. Marais, P. E. Badenhorst, A. Eksteen and Z. A. Pretorius, “Reduction of Aegilops sharonensis Chromatin Associated with Resistance Genes Lr56 and Yr38 in Wheat,” Euphytica, Vol. 171, No. 1, 2010, pp. 15-22.
[24] V. Kuraparthy, S. Sood, D. R. See and B. S. Gill, “Development of a PCR Assay and Marker-Assisted Transfer of Leaf Rust and Stripe Rust Resistance Genes Lr57 and Yr40 into Hard Red Winter Wheats,” Crop Science, Vol. 49, No. 1, 2009, pp. 120-126. doi:10.2135/cropsci2008.03.0143
[25] G. F. Marais, B. Mccallum and A. S. Marais, “Wheat Leaf Rust Resistance Gene Lr59 Derived from Aegilops peregrin,” Plant Breeding, Vol. 127, No. 4, 2008, pp. 340-345. doi:10.1111/j.1439-0523.2008.01513.x
[26] F. Marais, A. Marais, B. Mccallum and Z. Pretorius, “Transfer of Leaf Rust and Stripe Rust Resistance Genes Lr62 and Yr42 from Aegilops neglecta Req. ex Bertol. to Common Wheat,” Crop Science, Vol. 49, No. 3, 2009, pp. 871-879. doi:10.2135/cropsci2008.06.0317
[27] G. F. Marais, T. A. Bekker, A. Eksteen, B. Mccallum, T. Fetch and A. S. Marais, “Attempts to Remove Gametocidal Genes Co-Transferred to Common Wheat with Rust Resistance from Aegilops speltoides,” Euphytica, Vol. 171, No. 1, 2010, pp. 71-85.

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.