In vitro differentiation of human umbilical cord-derived mesenchymal stem cells into CD34⁺ cells via CD34 antibody

Shengnan Guo, Libin Guo, Maoxiang Sun, Wenyue Ma, Yujia Lu, Ying Liu

Department of Stem Cell Clinical Application Central, Siping Hospital Affiliated to China Medical University, Siping, China.
Department of Stem Cell Clinical Application Central, Siping Hospital Affiliated to China Medical University, Siping, China Tuhua Bioengineering Company Ltd., Siping, China.

DOI: 10.4236/jbise.2013.68A1005                

Abstract

CD34⁺cells differentiated from mesenchymal stem cells (MSCs) have a strong biological function in cardiovascular regeneration. However, the molecular mechanisms of and the methods to improve the CD34⁺ cell differentiation from MSCs, especially from human MSCs (hUC-MSCs) are still unclear. In the current study, the effect of CD34 antibody on the CD34⁺ cell differentiation from human umbilical cord (UC)-derived MSCs (hUC-MSCs) is determined. The results have demonstrated that the expression of cd34 protein is significantly increased in hUC-MSCs treated with CD34 antibody. In addition, the cell proliferation is increased in hUC-MSCs after treatment with CD34 antibody. Moreover, the expression of PI3K, AKT, p-AKT proteins, which are signaling molecules related to stem cell differentiation, is increased by CD34 antibody. The results suggest that CD34 antibody could promote the differentiation of hUC-MSCs into CD34⁺ cells and PI3K/AKT may be involved in this important process.

Keywords

Differentiation; Human Umbilical Cord-Derived Mesenchymal Stem Cells; CD34⁺ Cells

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Qiu, X., Sun, C., Yu, W., Lin, H., Sun, Z., Chen, Y., Wang, R. and Dai, Y. (2012) Combined strategy of mesenchymal stem cell injection with vascular endothelial growth factor gene therapy for the treatment of diabetes-associated erectile dysfunction. Journal of Andrology, 33, 37-44. doi:10.2164/jandrol.110.012666
[2]	Radhakrishnan, V., Muthurangan, M., May, A.-N., Balamuthu, K., Abdullah, A., Nehad, M.A. (2012) In vitro differentiation of human skin-derived multipotent stromal cells into putative endothelial-like cells. BMC Developmental Biology, 12, 7. doi:10.1186/1471-213X-12-7
[3]	Uccelli, A., Moretta, L. and Pistoia, V. (2008) Mesenchymal stem cells in health and disease. Nature Reviews Immunology, 8, 726-736. doi:10.1038/nri2395
[4]	Oswald, J., Boxberger, S., Jorgensen, B., Feldmann, S., Ehninger, G., Bornhauser, M. and Werner, C. (2004) Mesenchymal stem cells can be differentiated into endothelial cells in vitro. Stem Cells, 22, 377-384. doi:10.1634/stemcells.22-3-377
[5]	Pittenger, M.F., Mackay, A.M., Beck, S.C., Jaiswal, R.K., Douglas, R., Mosca, J.D., Moorman, M.A., Simonetti, D.W., Craig, S. and Marshak, D.R. (1999) Multilineage potential of adult human mesenchymal stem cells. Science, 284, 143-147. doi:10.1126/science.284.5411.143
[6]	Lian, Q., Zhang, Y., Zhang, J., Zhang, H.K., Wu, X., Lam, F.F., Kang, S., Xia, J.C., Lai, W.H., Au, K.W., Chow, Y.Y., Siu, C.W., Lee, C.N. and Tse, H.F. (2010) Functional mesenchymal stem cells derived from human induced pluripotent stem cells attenuate limb ischemia in mice. Circulation, 121, 1113-1123. doi:10.1161/CIRCULATIONAHA.109.898312
[7]	Liu, Z.J., Zhuge, Y. and Velazquez, O.C. (2009) Trafcking and differentiation of mesenchymalfi stem cells. Journal of Cellular Biochemistry, 106, 984-991. doi:10.1002/jcb.22091
[8]	Qu, Z.G., Guo, L.B., Fang, G.J., Cui, Z.H., Guo, S.N. and Liu, Y. (2012) Biological characteristics and effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) grafting with blood plasma on bone regeneration in rats. Cell Biochemistry and Biophysics, 63, 171-181. doi:10.1007/s12013-012-9354-1
[9]	Timmermans, F., Van Hauwermeiren, F., De Smedt, M., Raedt, R., Plasschaert, F., De Buyzere, M.L., Gillebert, T.C., Plum, J. and Vandekerckhove, B. (2007) Endothelial outgrowth cells are not derived from CD133+ cells or CD45+ hematopoietic precursors. Arteriosclerosis, Thrombosis, and Vascular Biology, 7, 1572-1579. doi:10.1161/ATVBAHA.107.144972
[10]	Zhang, Y., Fisher, N., Newey, S.E., Smythe, J., Tatton, L., Tsaknakis, G., Forde, S.P., Carpenter, L., Athanassopoulos, T., Hale, S.J., Ferguson, D.J., Tyler, M.P. and Watt, S.M. (2009) The impact of proliferative potential of umbilical cord-derived endothelial progenitor cells and hypoxia on vascular tubule formation in vitro. Stem Cells and Development, 18, 359-375. doi:10.1089/scd.2008.0071
[11]	Wu, K.H., Sheu, J.N., Wu, H.P., Tsai, C., Sieber, M., Peng, C.T. and Chao, Y.H. (2013) Cotransplantation of umbilical cord-derived mesenchymal stem cells promote hematopoietic engraftment in cord blood transplantation: A pilot study. Transplantation, 95, 773-777. doi:10.1097/TP.0b013e31827a93dd
[12]	Fan, C.G., Zhang, Q.J. and Zhou, J.R. (2011) Therapeutic potentials of mesenchymal stem cells derived from human umbilical cord. Stem Cells and Development, 7, 195-207. doi:10.1007/s12015-010-9168-8
[13]	Bayat, H., Fathi, F., Peyrovi, H. and Mowla, S.J. (2013) Evaluating the expression of self-renewal genes in human endothelial progenitor cells. Cell Journal, 14, 298-305.
[14]	Cheng, C.C., Chang, S.J., Chueh, Y.N., Huang, T.S., Huang, P.H., Cheng, S.M., Tsai, T.N., Chen, J.W. and Wang, H.W. (2013) Distinct angiogenesis roles and surface markers of early and late endothelial progenitor cells revealed by functional group analyses. BMC Genomics, 15, 14-182.
[15]	Stuart, J., Smith, Hanna, T., Jennifer, H.W., Donald, C.M., James, L., Beth, C. and Richard, G.G. (2012) CD105 (Endoglin) exerts prognostic effects via its role in the microvascular niche of paediatric high grade glioma. Acta Neuropathologica, 124, 99-110. doi:10.1007/s00401-012-0952-1
[16]	Jason, D.R., Rajendra, S.-M., Matthew, P.B., Steven, M.J., Lesley, D., Deepak, A.R., Tai, Y., Tamar, L.M., Ani, N., Brooks, U., Narutoshi, H., Toshiharu, S., Saltzman, W.M., Edward, S., Themis, R.K., Jordan, S.P., Christopher, K.B. (2010) Tissue-engineered vascular grafts transform into mature blood vessels via an inflammation-mediated process of vascular remodeling. Proceedings of the National Academy of Sciences of the USA, 107, 4669-4674.
[17]	Bellacosa, A., Kumar, C.C., Di Cristofano, A. and Testa, J.R. (2005) Activation of AKT kinases in cancer: Implications for therapeutic targeting. Advanced Cancer Research, 94, 29-86. doi:10.1016/S0065-230X(05)94002-5
[18]	Boomsma, R.A. and Geenen, D.L. (2012) Mesenchymal stem cells secrete multiple cytokines that promote angiogenesis and have contrasting effects on chemotaxis and apoptosis. PLoS One, 7, e35685. doi:10.1371/journal.pone.0035685