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Total Phenolic Content and Antioxidant Activity of Standardized Extracts from Leaves and Cell Cultures of Three Callistemon Species

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DOI: 10.4236/ajps.2011.26100    9,843 Downloads   16,982 Views   Citations


A comparative study was carried out with ethanolic (80%) extracts from leaves and cell cultures of three Callistemon species, namely C. lanceolatus (CL), C. viridiflorous (CV), and C. comboynensis (CC). Cell suspensions of the three species were grown in liquid Murashige and Skoog (MS) medium (100 ml) supplemented with 0.9 mg·g-1 kinetin in combination with 1.1 mg·g-1 NAA. The CL leaf extract was standardized to contain the highest amount of phenolics (104 ± 2.0 mg·g-1), followed by CC (95.8 ± 1.2 mg·g-1) and CV (79.8 ± 4.6 mg·g-1). On the other hand, cell cultures of CV contained more phenolics (14.9 ± 0.6 mg·g-1) than those of the other two species, CL and CC, which contained 12.2 ± 0.16 and 9.12 ± 0.16 mg·g-1, respectively. Nevertheless, CV leaf extract exhibited the highest antioxidant activity (91.4% ± 0.4%) at a concentration of 1000 µg·ml-1, comparable to 100 µg·ml-1 gallic acid (90.8% ± 1.5%).

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M. Abdelhady, A. Motaal and L. Beerhues, "Total Phenolic Content and Antioxidant Activity of Standardized Extracts from Leaves and Cell Cultures of Three Callistemon Species," American Journal of Plant Sciences, Vol. 2 No. 6, 2011, pp. 847-850. doi: 10.4236/ajps.2011.26100.


[1] P. C. Kanjilal and A. Das, “Flora of Assam,” Omsons Publications, New Delhi, 1992.
[2] I. I. Mahmoud, F. A. Moharram, M. S. Marzouk, M. W. Linscheid and M. I. Salch, “Polyphenolic Constituents of Callistemon Lanceolatus Leaves,” Pharmazie, Vol. 57, No. 7, 2002, pp. 494-496.
[3] R. K. Sharma, R. Kotoky and P. R. Bhattacharya, “Volatile Oil from the Leaves of Callistemon lanceolatus D.C. Grown in Northeastern India,” Flavonoid and Fragrance Journal, Vol. 21, No. 2, 2006, pp. 239-240.
[4] M. R. Ahuja, D. A. Evens, W. R. Sharp and P. J. Ammirato, “Handbook of Plant Cell Culture,” Macmillan, New York, 1986, pp. 626-651.
[5] A. Parsaeimehr, E. Sargsyan and K. Javidnia, “A Comparative Study of the Antibacterial, Antifungal and Antioxidant Activity and Total Content of Phenolic Compounds of Cell Cultures and Wild Plants of Three Endemic Species of Ephedra,” Molecules, Vol. 15, No. 3, 2010, pp. 1668-1678.
[6] E. A. Bell, “The Possible Significance of Secondary Compounds in Plant,” In: E. A. Bell and B. V. Charlwood, Eds., Secondary Plant Products, Springer-Verlag, New York, 1980, pp.11-21.
[7] F. Constable, O. L. Gamborg, W. G. W. Kurz and W. Steek, “Production of Secondary Metabolites in Plant Cell Cultures,” Planta Medica, Vol. 25, 1974, pp. 158-165.
[8] C. A. Rice-Evans, N. J. Miller and G. Paganaga, “Antioxidant Properties of Phenolic Compounds,” Trends in Plant Science, Vol. 2, No. 4, 1997, pp. 152-159.
[9] C. Zollman and A. Vickers, “Complementary Medicine and the Patient,” British Medical Journal, Vol. 319, 1999, pp. 1486-1494.
[10] M. K. Ang-Lee, S. J. Moss and C. S. Yuan, “P.P. Herbal Medicines and Preoperative Care,” The Journal of the American Medical Association, Vol. 286, No. 2, 2001, pp. 208-216.
[11] A. Wojdylo, J. Oszmianski and R. Czemerys, “Antioxidant Activity and Phenolic Compounds in 32 Selected Herbs,” Food Chemistry, Vol. 105, No. 3, 2007, pp. 940-949.
[12] R. S. D. Paul Raj, S. M. Morais and K. Gopalakrishnan, “In Vitro Propagation of Callistemon citrinus. L.,” Indian Journal of Science and Technology, Vol. 3, No. 1, 2010, p. 67.
[13] L. L. Mensor, F. S. Menezes, G. G. Leit?o, A. S. Reis, T. C. dos Santos, C. S. Coube and S. G. Leit?o, “Screening of Brazilian Plant Extracts for Antioxidant Activity by the Use of DPPH Free Radical Method,” Phytotherapy Research, Vol. 15, No. 2, 2001, pp. 127-130.
[14] S. Mcdonald, P. D. Prenzler, M. Autolovich and K. Robards, “Phenolic Content and Antioxidant Activity of Olive Oil Extracts,” Food Chemistry, Vol. 73, No. 1, 2001, pp. 73-84.
[15] A. Bendini, L. Cerretani, L. Pizzolante, T. Gallina-Toschi, F. Guzzo, S. Ceoldo, A. M. Marconi, F. Andreetta and M. Levi, “Phenol Content Related to Antioxidant and Antimicrobial Activity of Passiflora Spp. Extracts,” European Food Research and Technology, Vol. 223, No. 1, 2006, pp. 102-109.
[16] A. Dlugosz, J. Lembas-Bogaczyk and E. Lamer-Zarawska, “Antoxid Increases Ferric Reducing Antioxidant Power (FRAP) even Stronger than Vitamin C,” Acta Poloniae Pharmaceutica, Vol. 63, 2006, pp. 446-448.
[17] R. Harish and T. Shivanandappa, “Antioxidant Activity and Hepatoprotective Potential of Phyllanthus niruri,” Food Chemistry, Vol. 95, No. 2, 2006, pp. 180-185.
[18] N. Hassimotto, M. Genovese and F. Lajolo, “Antioxidant Activity of Dietary Fruits, Vegetables, and Commercial Frozen Fruit Pulps,” Journal of Agricultural and Food Chemistry, Vol. 53, No. 8, 2005, pp. 2928-2935.
[19] D. J. Newman, G. M. Gragg, S. Holbeck and E. A. Sausville, “Natural Products as Leads to Cell Cycle Pathway Targets in Cancer Chemotherapy,” Current Cancer Drug Targets, Vol. 2, No. 4, 2002, pp. 279-308.
[20] V. Rajkumar, H. Guha and R. A. Kumar, “Antioxidant and Anticancer Potentials of Rheum Emodi Rhizome Extracts,” Food and Chemical Toxicology, Vol. 49, No. 2, 2011, pp. 363-369.

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