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Assessment of Antifungal Activity of Some Himalayan Foliose Lichens Against Plant Pathogenic Fungi

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DOI: 10.4236/ajps.2011.26099    5,701 Downloads   11,427 Views   Citations

ABSTRACT

In vitro antifungal activity of the acetone, methanol and chloroform extracts of four lichen species viz, Bulbothirx setschwanensis, Everniastrum nepalense, Heterodermia diademata, Parmelaria thomsonii were investigated against seven plant pathogenic fungi (Aspergillus flavus, A. fumigatus, Alternaria alternata, Fusarium oxysporum, F. solani, F. roseum and Penicillium citrinum) with reference to commercially available synthetic antifungal drug Ketoconazole (positive control). Lichen secondary metabolites were extracted using Soxhlet extractor and were further recovered through gentle evaporation of solvents in rotatory evaporator. Antifungal activity was analysed employing BauerKirby disc diffusion assay. Acetone and methanol extracts of lichenized fungi were found more effective against tested plant pathogenic fungi. Principal component analysis concluded that though, Ketoconazole was effective against four of the tested plant pathogenic fungi, acetone and methanol extracts of lichens were comparatively more effective against some broad spectrum plant pathogenic fungi (Fusarium oxysporum, F. solani, F. roseum).

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

P. Tiwari, H. Rai, D. Upreti, S. Trivedi and P. Shukla, "Assessment of Antifungal Activity of Some Himalayan Foliose Lichens Against Plant Pathogenic Fungi," American Journal of Plant Sciences, Vol. 2 No. 6, 2011, pp. 841-846. doi: 10.4236/ajps.2011.26099.

References

[1] M. E. Hale, “The Biology of Lichens,” 3rd Edition, Edward Arnold Ltd., London, 1983.
[2] K. O. Vartia, “Antibiotics in Lichens,” In: V. Ahmadjian and M. E. Hale, Eds., The Lichens, Academic Press, New York, 1973, pp. 547-561.
[3] D. Fahselt, “Secondary Biochemistry of Lichens,” Symbiosis, Vol. 16, No. 2, 1994, pp. 117-165.
[4] K. Müller, “Pharmaceutically Relevant Metabolites from Lichens,” Applied Microbiology and Biotechnology, Vol. 56, No. 1-2, 2001, pp. 9-12. doi:10.1007/s002530100684
[5] K. Ingolfsd?ttir, G. A. C. Chung, S. R. Gissurarson, V. G. Skulason and M. Vilhelmsdottir, “In vitro Antimycobacterial Activity of Lichen Metabolites,” European Journal of Pharmaceutical Sciences, Vol. 6, No. 2, 1998, pp. 141-144. doi:10.1016/S0928-0987(97)00078-X
[6] J. A. Elix and E. Stocker-Worgotter, “Biochemistry and Secondary Metabolites,” In: T. H. Nash III, Ed., Lichen biology, Cambridge University Press, Cambridge, 2008, pp. 104-133. doi:10.1017/CBO9780511790478.008
[7] B. Rankovi? and M. Mi?i?, “Antifungal Activity of Extract of the Lichens Alectoria Sarmentosa and Cladonia Rangiferina,” Mikologijai Fitopatologija, Vol. 41, 2007, pp. 276-281.
[8] K. Ingólfsdóttir, G. F. Gudmundsdóttir, H. M. ?gmundsdóttir, K. Paulus, S. Haraldsdóttir, H. Kristinsson and R. Bauer, “Effects of Tenuiorin and Methyl Orsellinate from the Lichen Peltigera leucophlebia on 5-/15-Lipoxygensases and Proliferation of Malignant Cell Lines in Vitro,” Phytomedicine, Vol. 9, 2002, pp. 654-658. doi:10.1078/094471102321616481
[9] M. Gulluce, A. Aslan, M. Sokmen, F. Sahin, A. Adiguzel, G. Agar and A. Sokmen “Screening the Antioxidant and Antimicrobial Properties of the Lichens Parmelia saxatilis, Platismatia glauca, Ramalina pollinaria, Ramalina polymorpha and Umbilicaria nylanderiana,” Phytomedicine, Vol. 13, No. 7, 2006, pp. 515-521. doi:10.1016/j.phymed.2005.09.008
[10] I. Karaman, F. Sanin, M. Güllüce, H. Ogütcü, M. Sengul and A. Adigüzel, “Antimicrobial Activity of Aquaeos and Methanol Extracts of Juniperus oxicedrus,” Journal of Ethnopharmacology, Vol. 37, No. 2-3, 2003, pp. 1-5.
[11] K. Hostettmann, L. Wolfender and S. Rodriguez, “Rapid Detections and Subsequent Isolation of Bioactive Constituents of Crude Plant Extracts,” Planta Medica, Vol. 63, No. 1, 1987, pp. 2-10. doi:10.1055/s-2006-957592
[12] A. Orange, P. W. James and F. J. White, “Microchemical Methods for the Identification of Lichen Products,” British Lichen Society, Edinburgh, 2001.
[13] J. A. Elix and J. L. Ernst-Russel, “A Catalogue of Standardized Thin Layer Crhomatographic Data and Biosynthetic Relationships for Lichen Substances,” 2nd Edition, Australian National University, Canberra, 1993.
[14] D. D. Awasthi, “A Compendium of the Macrolichens from India, Nepal and Sri Lanka,” Bishen Singh Mahendra Pal Singh, Dehra-Dun, 2007.
[15] P. K Divakar and D. K Upreti, “Parmelioid Lichens in India: A Revisionary Study,” Bishen Singh Mahendra Pal Singh., Dehra-Dun, 2005.
[16] F. Soxhlet, “Die Gewichtsanalytische Bestimmung des Milchfettes,” Polytechnisches Journal, Vol. 232, 1879, pp. 461-465.
[17] L. M. Harwood and C. J. Moody, “Experimental Organic Chemistry: Principles and Practice,” Illustrated Edition, Blackwell Scientific, Oxford, 1989.
[18] A. W. Bauer, D. M. Perry and W. M. M. Kirby, “Single Disc Antibiotic Sensitivity Testing of Staphylococci,” Achieve of Internal Medicine, Vol. 104, No. 2, 1959, pp. 208-216.
[19] A. W. Bauer, W. M. M. Kirby, J. K. C. Scherris and M. Turck, “Antibiotic Susceptibility Testing by a Standardized Single Disc Method,” American Journal of Clinical Pathology, Vol. 45, No. 4, 1966, pp. 493-496.
[20] J. M. Larkin, “A Laboratory Manual for Microbiology,” 3rd Edition, Kendal/Hunt Publishing Company, San Francisco, 1982.
[21] W. M. M. Kirby, G. M. Yoshihara, K. Sundstedt and J. Warren, “Clinical Usefulness of a Single Disc Method for Antibiotic Sensitivity Testing,” Antibiotics Annual 19561957, Antibiotica, Inc., New York, 1957, p. 892.
[22] National Committee for Clincal Laboratory Standards, NCCLS Document 1997, M26-P Villanova.
[23] C. J. F. ter Braak and I. C. Prentice, “A Theory of Gradient Analysis,” Advances in Ecological Research, Vol. 18, 1988, pp. 271-313. doi:10.1016/S0065-2504(08)60183-X
[24] H. G. Gauch Jr., “Multivariate Analysis in Community Structure,” Cambridge University Press, Cambridge, 1982.
[25] ?. Hammer, D. A. T. Harper and D. P. Ryan, “PAST: Paleontological Statistics Software Package for Education and Data Analysis,” Palaentologia Electonica, Vol. 4, 2001, pp. 1-9.
[26] P. Tiwari, H. Rai, D. K. Upreti, S. Trivedi and P. Shukla, “Antifungal Activity of a Common Himalayan Foliose Lichen Parmotrema tinctorum (Despr. ex Nyl.) Hale,” Nature and Science, Vol. 9, No. 9, 2011, pp. 167-171.
[27] S. Huneck, “The Significance of Lichens and Their Metabolites,” Naturwissenschaften, Vol. 86, No. 12, 1999, pp. 559-570. doi:10.1007/s001140050676
[28] P. Halama and C. Van Haluwin, “Antifungal Activity of Lichen Extracts and Lichenic Acids,” Biocontrol, Vol. 49, No. 1, 2004, pp. 95-107. doi:10.1023/B:BICO.0000009378.31023.ba
[29] M. Goel, P. Dureja, A. Rani, P. L. Uniyal and H. Laatsch, “Isolation, Characterization and Antifungal Activity of Major Constituents of the Himalayan Lichen Parmelia reticulata Tayl.,” Journal of Agriculture and Food Chemistry, Vol. 59, No. 6, pp. 2299-2307.

  
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