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Uncoupling protein 2 regulates myocardial apoptosis via the diabetogenic action of streptozotocin

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DOI: 10.4236/jbise.2011.47064    3,081 Downloads   5,989 Views   Citations

ABSTRACT

Objective: Determine the role of uncoupling protein 2 (UCP2) in the myocardial apoptosis of diabetic mellitus(DM). Methods: DM animal models were induced by streptozotocinon (STZ) on UCP2 knock-out mice (UCP2KO) and wild-type mice (WT), which were reared for 7 and 28 days after successful modeling, respectively. The expressions of relative protein for myocardial apoptosis, pro-caspase-9, were investigated using western blot. However, the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) was used to explain apoptosis at the DNA level. Results: Image analysis showed that the expression of pro-caspase-9 protein levels increased slightly in UCP-/- + DM-7-day group comparing with DM-7-day group (P > 0.05). The expression of pro-caspase-9 protein levels increased significantly (P < 0.05)in UCP-/- + DM-28-day group comparing with DM-28-day group. TUNEL analysis indicated that UCP2 reduced the number of apoptotic myocytes in the DM-28-day group by 70% in comparison to DM-7-day group by 30% (P < 0.05). Conclusion UCP2 may be one of the most important factors that contribute to the myocardial apoptosis of DM.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Li, X. , Tan, R. and Lu, X. (2011) Uncoupling protein 2 regulates myocardial apoptosis via the diabetogenic action of streptozotocin. Journal of Biomedical Science and Engineering, 4, 506-510. doi: 10.4236/jbise.2011.47064.

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