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Evaluation of the Anti-Diabetic Potential of the Methanol Extracts of Aloe camperi, Meriandra dianthera and a Polyherb

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DOI: 10.4236/jdm.2015.54033    3,771 Downloads   4,318 Views   Citations

ABSTRACT

The objective of the study was to evaluate the anti-diabetic activities of methanol extracts of Aloe camperi (AC), Meriandra dianthera (MD) and a polyherbal drug (PH) in diabetes induced Wistar albino rats. A single dose of alloxan monohydrate (150 mg/kg, i.p.) was used to induce diabetes mellitus (DM). Diabetes was confirmed by the elevated blood glucose levels determined after 72 h of induction. Animals with mean fasting blood glucose (FBG) level more than 200 mg/dl were recruited for the experiment. The herbal extracts at doses of 200 and 400 mg/kg and standard drugmetformin (5 mg/kg) were administered orally to the diabetic rats for 21 days and the FBG level was estimated on 0, 7, 14 and 21 days. The herbal extracts showed dose-dependent fall in FBG levels and the result exhibited very significant (P < 0.001) decreases in FBG level by the end of the experimental day as compared to the diabetic control. The highest antihyperglycemic effect was observed by MD extract at 400 mg/kg and was comparable to the standard drug. Oral glucose tolerance test (OGTT) was also conducted on normal rats and thus glucose at 2 g/kg per body weight was loaded via oral gavage to all groups 30 min after extract administration. All the groups showed significant increase (P < 0.01 or P < 0.05) in FBG level at 30 min following glucose loading. The hyperglycemia with glucose challenge was significantly brought down (P < 0.001) by all herbal extracts at 60 and 120 min relative to the negative control. Moreover, acute oral toxicity tests was conducted based on the protocols of OECD-425 and thus the LD50 of the herbal extracts was estimated to be greater than 2000 mg/kg. Statistical analysis was performed using One-Way ANOVA followed by Dunnett’s test for multiple comparisons, and values of P < 0.05 were considered as statistically significant.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Mussie, S.D., Kareru, P.G., Kericko, J.M., and Berhane, G.N. (2015) Evaluation of the Anti-diabetic Potential of the Methanol Extracts of Aloe camperi, Meriandra dianthera and a Polyherb. Journal of Diabetes Mellitus.

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