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Plasma Citrulline: A New Marker of Gut Epithelium Alteration in Obese Patients?

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DOI: 10.4236/jdm.2015.54028    3,526 Downloads   3,931 Views  

ABSTRACT

Objectives: In the last decade gut microbial diversity was associated with the pathogenesis of obesity in humans. Plasma citrulline was a simple and accurate biomarker for the severity of intestinal failure and was associated with short bowel syndrome and alteration of gut permeability, being developed as an alternative to D-xylose tolerance test for the diagnosis of an abnormal small intestinal absorption of nutrients. This study was performed to ascertain whether obesity might be associated with dysregulation of epithelial gut function. Methods: Fifteen obese individuals (5 M/10 F; BMI 37.4 ± 6.1 Kg/m2; 42 ± 6 yrs) and 15 healthy gender- and age-matched controls (6 M/9 F BMI: 22.7 ± 2.1 Kg/m2; 39 ± 7 yrs) underwent D-xylose load (25 g) and plasma citrulline, plasma insulin, glucose and lipid profile testing. Results: Plasma citrulline was significantly lower in the obese group (p = 0.045) with respect to controls, whilst total cholesterol, LDL and trygliceri- des concentration, insulin level and HOMA-IR were significantly higher in obese patients. In contrast, after D-xylose load no difference in serum xylose was found between the two groups (p = ns). Conclusions: Obese patients show a decreased citrulline concentration with respect to lean subjects. Since citrulline is a known marker of intestinal health, alterations in the gut epithelium are likely to be associated with the obesity syndrome. We propose to measure citrulline level in obese patients on a routine basis.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Benedini, S. , Fermo, I. , Caumo, A. , Terruzzi, I. and Luzi, L. (2015) Plasma Citrulline: A New Marker of Gut Epithelium Alteration in Obese Patients?. Journal of Diabetes Mellitus, 5, 233-237. doi: 10.4236/jdm.2015.54028.

References

[1] DiBaise, J.K., Zhang, H., Crowell, M.D., Krajmalnik-Brown, R., Decker, G.A. and Rittmann, B.E. (2008) Gut Microbiota and Its Possible Relationship with Obesity. Mayo Clinic Proceedings, 83, 460-469.
http://dx.doi.org/10.4065/83.4.460
[2] Backhed, F., Ley, R.E., Sonnenburg, J.L., Peterson, D.A. and Gordon, J.I. (2005) Host-Bacterial Mutualism in the Human Intestine. Science, 307, 1915-1920.
http://dx.doi.org/10.1126/science.1104816
[3] Gong, J.F., Zhu, W.M., Li, N., Liu, F.N., Tan, L., Luo, N. and Li, J.S. (2005) Serum Citrulline Is a Simple Quantitative Marker for Small Intestinal Enterocytes Mass and Absorption Function in Short Bowel Patients. Journal of Surgical Research, 127, 177-182.
http://dx.doi.org/10.1016/j.jss.2005.04.004
[4] Ehrenpreis, E.D., Salvino, M. and Craig, R.M. (2001) Improving the Serum D-Xylose Test for the Identification of Patients with Small Intestinal Malabsorption. Journal of Clinical Gastroenterology, 33, 36-40.
http://dx.doi.org/10.1097/00004836-200107000-00009
[5] Fermo, I., De Vecchi, E., Diomede, L. and Paroni, R. (1990) Serum Amino Acid Analysis with Pre-Column Derivatization: Comparison of the o-Phthaldialdehyde and N,N-Diethyl-2,4-dinitro-5-fluoroaniline Methods. Journal of Chromatography B, 534, 23-35.
http://dx.doi.org/10.1016/S0378-4347(00)82145-3
[6] Turnbaugh, P.J., Ley, R.E., Mahowald, M.A., Magrini, V., Mardis, E.R. and Gordon, J.I. (2006) An Obesity-Associated Gut Microbiome with Increased Capacity for Energy Harvest. Nature, 444, 1027-1031.
[7] David, A.I., Selvaggi, G., Ruiz, P., Gaynor, J.J., Tryphonopoulos, P., Kleiner, G.I., Moon, J.I., Nishida, S., Pappas, P.A., Conanan, L., Weppler, D., Esquenazi, V., Levi, D.M., Kato, T. and Tzakis, A.G. (2007) Blood Citrulline Level Is an Exclusionary Marker for Significant Acute Rejection after Intestinal Transplantation. Transplantation, 84, 1077-1081.
[8] Batista, M.A., Nicoli, J.R., Martins, F.S., Machado, J.A.N., Arantes, R.M.E., Quirino, I.E.P., et al. (2012) Pretreatment with Citrulline Improves Gut Barrier after Intestinal Obstruction in Mice. Journal of Parenteral and Enteral Nutrition, 36, 69-76.
http://dx.doi.org/10.1177/0148607111414024
[9] Tennyson, C.A. and Friedman, G. (2008) Microecology, Obesity, and Probiotics. Current Opinion in Endocrinology, Diabetes & Obesity, 15, 422-427.
http://dx.doi.org/10.1097/MED.0b013e328308dbfb
[10] Brun, P., Castagliuolo, I., Di Leo, V., Buda, A., Pinzani, M., Palu, G. and Martines, D. (2007) Increased Intestinal Permeability in Obese Mice: New Evidence in the Pathogenesis of Nonalcoholic Steatohepatitis. American Journal of Physiology-Gastrointestinal and Liver Physiology, 292, G518-G525.
http://dx.doi.org/10.1152/ajpgi.00024.2006
[11] Osbak, P.S., Bindslev, N. and Hansen, M.B. (2011) Relationships between Body Mass Index and Short-Circuit Current in Human Duodenal and Colonic Mucosal Biopsies. Acta Physiologica (Oxford), 201, 47-53.
http://dx.doi.org/10.1111/j.1748-1716.2010.02202.x

  
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