Share This Article:

Biodegradation of Linear Alkylbenzene Sulfonate (LAS) by Immobilized Pseudomonas sp.

Abstract Full-Text HTML XML Download Download as PDF (Size:516KB) PP. 465-475
DOI: 10.4236/aces.2015.54048    3,752 Downloads   4,296 Views   Citations

ABSTRACT

Anionic surfactants are the most widely exploited chemical surfactants, which are being incorporated into majority of detergents and cleaning products used for household and industrial applications. Linear alkylbenzene sulfonates (LAS) is one of the major xenobiotic anionic surfactants. Biodegradation is an effective process to reduce the amount of surfactants released in the environment. In this study soil samples were collected from detergent contaminated sites to isolate linear alkylbenzene sulphonate degrading bacteria using mineral salt media supplemented with LAS as sole source of carbon. From the twenty isolates selected for the study, two of them (L9 and L12) have exhibited excellent ability to degrade LAS. The LAS degradation ability was determined by using MBAS assay and HPLC. The selected isolates were immobilized in alginate and polyvinyl alcohol to check the suitability of the isolates in onsite LAS removal. The percentage of degradation of LAS by alginate entrapped L9 was 85.155 ± 1.2 and that of PVA immobilized cells was 58.535 ± 2.9. Whereas L12 PVA entrapped were good compared to alginate. L12 exhibited 62.977 ± 1.3 percentage of degradation of LAS when immobilized in PVA and 61.07 ± 0.6 percentage in alginate entrapped condition. In comparison between the organisms alginate entrapped Pseudomonas nitroreducens (L9) was found to be superior to Pseudomonas aeruginosa (L12) in immobilised condition. It was found that immobilized cells performed superiorly than free cells. In particular, calcium alginate immobilised cells were more efficient in LAS removal than polyvinyl alcohol immobilised cells.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Asok, A. , Fathima, P. and Jisha, M. (2015) Biodegradation of Linear Alkylbenzene Sulfonate (LAS) by Immobilized Pseudomonas sp.. Advances in Chemical Engineering and Science, 5, 465-475. doi: 10.4236/aces.2015.54048.

References

[1] Jensen, J. (1999) Fate and Effects of Linear Alkylbenzene Sulphonates (LAS) in the Terrestrial Environment. Science of the Total Environment, 226, 93-111.
http://dx.doi.org/10.1016/S0048-9697(98)00395-7
[2] Hullar, M.A.J., Kaplan, L.A. and Stahl, D.A. (2006) Recurring Seasonal Dynamics of Microbial Communities in Stream Habitats. Applied and Environmental Microbiology, 72, 713-722.
http://dx.doi.org/10.1128/AEM.72.1.713-722.2006
[3] Zhang, C.L., Valsaraj, K.T., Constant, W.D. and Roy, D. (1999) Aerobic Biodegradation Kinetics of Four Anionic and Nonionic Surfactants at Sub- and Supra-Critical Micelle Concentrations (CMCs). Water Research, 33, 115-124.
http://dx.doi.org/10.1016/S0043-1354(98)00170-5
[4] Mösche, M. and Meyer, U. (2002) Toxicity of Linear Alkylbenzene Sulfonate in Anaerobic Digestion: Influence of Exposure Time. Water Research, 36, 3253-3260.
http://dx.doi.org/10.1016/S0043-1354(02)00034-9
[5] Dhouib, A., Hamad, N., Hassaïri, I. and Sayadi, S. (2003) Degradation of Anionic Surfactants by Citrobacter braakii. Process Biochemistry, 38, 1245-1250.
http://dx.doi.org/10.1016/S0032-9592(02)00322-9
[6] D’Souza, S.F. (2002) Trends in Immobilized Enzyme and Cell Technology. Indian Journal of Biotechnology, 1, 321-338.
[7] Sasaki, H., Nonaka, J., Sasaki, T. and Nakai, Y. (2007) Ammonia Removal from Livestock Wastewater by Ammonia-Assimilating Microorganisms Immobilized in Polyvinyl Alcohol. Journal of Industrial Microbiology & Biotechnology, 34, 105-110.
http://dx.doi.org/10.1007/s10295-006-0172-6
[8] Bandhyopadhyay, K., Das, D., Bhattacharyya, P. and Maiti, B.R. (2001) Reaction Engineering Studies on Biodegradation of Phenol by Pseudomonas putida MTCC 1194 Immobilized on Calcium Alginate. Biochemical Engineering Journal, 8, 179-186.
http://dx.doi.org/10.1016/S1369-703X(01)00101-2
[9] Martins, S.C.S., Martins, C.M., Fiúza, L.M.C.C. and Santaella, S.T. (2013) Immobilization of Microbial Cells: A Promising Tool for Treatment of Toxic Pollutants in Industrial Wastewater. African Journal of Biotechnology, 12, 4412-4418.
http://dx.doi.org/10.5897/AJB12.2677
[10] Hayashi, K. (1975) A Rapid Determination of Sodium Dodecyl Sulfate with Methylene Blue. Analytical Biochemistry, 67, 503-506.
http://dx.doi.org/10.1016/0003-2697(75)90324-3
[11] Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd Edition, Cold Spring Laboratory Press, Cold Spring Harbor, New York.
[12] Zhang, Z., Schwartz, S., Wagner, L. and Miller, W. (2000) A Greedy Algorithm for Aligning DNA Sequences. Journal of Computational Biology, 7, 203-214.
http://dx.doi.org/10.1089/10665270050081478
[13] Abouseoud, M., Yataghene, A., Amrane, A. and Maachi, R. (2008) Biosurfactant Production by Free and Alginate Entrapped Cells of Pseudomonas fluorescens. Journal of Industrial Microbiology & Biotechnology, 35, 1303-1308.
http://dx.doi.org/10.1007/s10295-008-0411-0
[14] Jeong, H.-S., Lim, D.-J., Hwang, S.-H., Ha, S.-D. and Kong, J.-Y. (2004) Rhamnolipid Production by Pseudomonas aeruginosa Immobilised in Polyvinyl Alcohol Beads. Biotechnology Letters, 26, 35-39.
http://dx.doi.org/10.1023/B:BILE.0000009457.42943.90
[15] Vives-Rego, J., López-Amorós, R., García, M.T., Comas, J. and Sánchez-Leal, J. (2000) Microbial Aspects of Linear Alkylbenzene Sulfonate Degradation in Coastal Water. Journal of Surfactants and Detergents, 3, 303-308.
http://dx.doi.org/10.1007/s11743-000-0133-0
[16] Maguire, J.D. (1962) Speed of Germination—Aid in Selection and Evaluation for Seedling Emergence and Vigor. Crop Science, 2, 176-177.
http://dx.doi.org/10.2135/cropsci1962.0011183X000200020033x
[17] Abdul-Baki, A.A. and Anderson, J.D. (1973) Vigor Determination in Soybean Seed by Multiple Criteria. Crop Science, 13, 630-633.
http://dx.doi.org/10.2135/cropsci1973.0011183X001300060013x
[18] Jurado, E., Fernández-Serrano, M., Núñez-Olea, J., Luzón, G. and Lechuga, M. (2006) Simplified Spectrophotometric Method Using Methylene Blue for Determining Anionic Surfactants: Applications to the Study of Primary Biodegradation in Aerobic Screening Tests. Chemosphere, 65, 278-285.
http://dx.doi.org/10.1016/j.chemosphere.2006.02.044
[19] Swisher, R.D. (1986) Surfactant Biodegradation. Vol. 18, CRC Press, Boca Raton.
[20] Thomas, O.R.T. and White, G.F. (1991) Immobilization of the Surfactant-Degrading Bacterium Pseudomonas C12B in Polyacrylamide Gel. III. Biodegradation Specificity for Raw Surfactants and Industrial Wastes. Enzyme and Microbial Technology, 13, 338-343.
http://dx.doi.org/10.1016/0141-0229(91)90154-3
[21] Tallur, P.N., Megadi, V.B. and Ninnekar, H.Z. (2009) Biodegradation of P-Cresol by Immobilized Cells of Bacillus sp. Strain PHN 1. Biodegradation, 20, 79-83.
http://dx.doi.org/10.1007/s10532-008-9201-7
[22] DomÃ-nguez, A., Couto, S.R.G. and Sanroman, M.A. (2005) Dye Decolorization by Trametes hirsuta Immobilized into Alginate Beads. World Journal of Microbiology and Biotechnology, 21, 405-409.
http://dx.doi.org/10.1007/s11274-004-1763-x
[23] Mohammad, B.T. and Bustard, M.T. (2008) Fed Batch Bioconversion of 2-Propanol by a Solvent Tolerant Strain of Alcaligenes faecalis Entrapped in Ca-Alginate Gel. Journal of Industrial Microbiology & Biotechnology, 35, 677-684.
http://dx.doi.org/10.1007/s10295-008-0325-x
[24] Carvalho, W., Silva, S.S., Santos, J.C. and Converti, A. (2003) Xylitol Production by Ca-Alginate Entrapped Cells: Comparison of Different Fermentation Systems. Enzyme and Microbial Technology, 32, 553-559.
http://dx.doi.org/10.1016/S0141-0229(03)00007-3
[25] Westmeier, F. and Rehm, H.J. (1985) Biodegradation of 4-Chlorophenol by Entrapped Alcaligenes sp. A 7-2. Applied Microbiology and Biotechnology, 22, 301-305.
http://dx.doi.org/10.1007/BF00582412
[26] Wijffels, R.H. (2001) Immobilized Cells. Springer Science & Business Media, Berlin.
http://dx.doi.org/10.1007/978-3-642-56891-6
[27] Robinson, T., McMullan, G., Marchant, R. and Nigam, P. (2001) Remediation of Dyes in Textile Effluent: A Critical Review on Current Treatment Technologies with a Proposed Alternative. Bioresource Technology, 77, 247-255.
http://dx.doi.org/10.1016/S0960-8524(00)00080-8
[28] Hosseini, F., Malekzadeh, F., Amirmozafari, N. and Ghaemi, N. (2007) Biodegradation of Anionic Surfactants by Isolated Bacteria from Activated Sludge. International Journal of Environmental Science & Technology, 4, 127-132.
http://dx.doi.org/10.1007/BF03325970
[29] Lal, N. and Mishra, R. (2003) Effect of Synthetic Detergent on Germination Parameters, Seedling Growth and Photosynthetic Pigments in Mungbean (Vigna Radiata) Seedlings. Pollution Research, 22, 335-337.

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.