Share This Article:

Genomic Fingerprinting of Camelina Species Using cTBP as Molecular Marker

Abstract Full-Text HTML XML Download Download as PDF (Size:1768KB) PP. 1184-1200
DOI: 10.4236/ajps.2015.68122    3,029 Downloads   3,527 Views   Citations

ABSTRACT

Interest on the genus Camelina has recently increased due to the biofuel, or jet fuel, potential of the oil extracted from seeds of the cultivated species Camelina sativa (L.) Crantz. While our knowledge on C. sativa is constantly augmenting, only few studies have been performed on the other species of the genus, which could be a potentially useful material for the genetic improvement of C. sativa. The genus Camelina consists of 11 species, but only six (C. sativa, C. microcarpa, C. alyssum, C. rumelica, C. hispida and C. laxa) could be retrieved from germplasm banks to carry out genomic fingerprinting studies based on the use of the cTBP molecular marker. Each species, with the exception of C. alyssum that is proposed to be a subspecies of C. sativa, shows a distinct cTBP profile resulting from multiple DNA length polymorphisms present in the second intron of the members of the β-tubulin gene family. In contrast to the high level of genetic diversity detected among the six Camelina species, low variability is observed among and within the accessions of the same species, except for C. hispida that is characterized by an intra-accession high number of cTBP polymorphic bands. In addition, cTBP is also able to identify incorrectly classified accessions and provide information on the ploidy level of each species.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Galasso, I. , Manca, A. , Braglia, L. , Ponzoni, E. and Breviario, D. (2015) Genomic Fingerprinting of Camelina Species Using cTBP as Molecular Marker. American Journal of Plant Sciences, 6, 1184-1200. doi: 10.4236/ajps.2015.68122.

References

[1] Warwick, S.I., Francis, A. and Al-Shehbaz, I.A. (2006) Brassicaceae: Species Checklist and Database on CD Rom. Plant Systematic and Evolution, 259, 249-258.
http://dx.doi.org/10.1007/s00606-006-0422-0
[2] Bernardo, A., Howard-Hildige, R., O’Connell, A., Ryan, J., Rice, B., Roche, E. and Leahy, J.J. (2003) Camelina Oil as a Fuel for Diesel Transport Engines. Industrial Crops and Products, 17, 191-197.
http://dx.doi.org/10.1016/S0926-6690(02)00098-5
[3] Fröhlich, A. and Rice, B. (2005) Evaluation of Camelina sativa Oil as a Feedstock for Biodiesel Production. Industrial Crops and Products, 21, 25-31.
http://dx.doi.org/10.1016/j.indcrop.2003.12.004
[4] Soriano, N.U. and Narami, A. (2012) Evaluation of Biodiesel Derived from Camelina sativa Oil. Journal of the American Oil Chemists’ Society, 89, 917-923.
http://dx.doi.org/10.1007/s11746-011-1970-1
[5] Zaleckas, E., Makareviciene, V. and Sendzikiene, E. (2012) Possibilities of Using Camelina sativa Oil for Producing Biodiesel Fuel. Transport, 27, 60-66.
http://dx.doi.org/10.3846/16484142.2012.664827
[6] Shonnard, D.R., Williams, L. and Kalnes, T.N. (2010) Camelina-Derived Jet Fuel and Diesel: Sustainable Advanced Biofuels. Environmental Progress & Sustainable Energy, 3, 382-392.
http://dx.doi.org/10.1002/ep.10461
[7] Agusdinata, D.B., Zhao, F., Ileleji, K. and De Laurentis, D. (2011) Life Cycle Assessment of Potential Biojet Fuel Production in the United States. Environmental Science & Technology, 45, 9133-9143.
http://dx.doi.org/10.1021/es202148g
[8] Zubr, J. (1997) Oil-Seed Crop: Camelina sativa. Industrial Crops and Products, 6, 113-119.
http://dx.doi.org/10.1016/S0926-6690(96)00203-8
[9] Rode, J. (2002) Study of autochthon Camelinasativa (L.) Crantz in Slovenia. Journal of Herbs, Spices & Medicinal Plants, 9, 313-318.
http://dx.doi.org/10.1300/J044v09n04_08
[10] Budin, J.T., Breene, W.M. and Putnam, D.H. (1995) Some Compositional Properties of Camelina (Camelina sativa (L.) Crantz) Seeds and Oils. Journal of the American Oil Chemists’ Society, 72, 309-315.
http://dx.doi.org/10.1007/BF02541088
[11] Zubr, J. and Matthäus, B. (2002) Effects of Growth Conditions on Fatty Acids and Tocopherols in Camelina sativa Oil. Industrial Crops and Products, 15, 155-162.
http://dx.doi.org/10.1016/S0926-6690(01)00106-6
[12] Abramovic, H., Butinar, B. and Nikolic, V. (2007) Changes Occurring in Phenolic Content, Tocopherol Composition and Oxidative Stability of Camelina sativa Oil during Storage. Food Chemistry, 104, 903-909.
http://dx.doi.org/10.1016/j.foodchem.2006.12.044
[13] Hrastar, R., Abramovic, H. and Kosir, I.J. (2012) In Situ Quality Evaluation of Camelina sativa Landrace. European Journal of Lipid Science and Technology, 114, 343-351.
http://dx.doi.org/10.1002/ejlt.201100003
[14] Gehringer, A., Friedt, W., Lühs, W. and Snowdon, R.J. (2006) Genetic Mapping of Agronomic Traits in False Flax (Camelina sativa Subsp. sativa). Genome, 49, 1555-1563.
http://dx.doi.org/10.1139/g06-117
[15] Vollmann, J., Grausgruber, H., Stift, G., Dryzhyruk, V. and Lelley, T. (2005) Genetic Diversity in Camelina germplasm as Revealed by Seed Quality Characteristics and RAPD Polymorphism. Plant Breeding, 124, 446-453.
http://dx.doi.org/10.1111/j.1439-0523.2005.01134.x
[16] Ghamkhar, K., Croser, J., Aryamanesh, N., Campbell, M., Kon’kova, N. and Francis, C. (2010) Camelina (Camelina sativa (L.) Crantz) as an Alternative Oilseed: Molecular and Ecogeographic Analyses. Genome, 53, 558-567.
http://dx.doi.org/10.1139/G10-034
[17] Galasso, I., Manca, A., Braglia, L., Martinelli, T., Morello, L. and Breviario, D. (2011) h-TBP: An Approach Based on Intron-Length Polymorphism for the Rapid Isolation and Characterization of the Multiple Members of the β-Tubulin Gene Family in Camelina sativa (L.) Crantz. Molecular Breeding, 28, 635-645.
http://dx.doi.org/10.1007/s11032-010-9515-0
[18] Manca, A., Pecchia, P., Mapelli, S., Masella, P. and Galasso, I. (2012) Evaluation of Genetic Diversity in a Camelina sativa (L.) Crantz Collection Using Microsatellite Markers and Biochemical Traits. Genetic Resources and Crop Evolution, 60, 1223-1236.
http://dx.doi.org/10.1007/s10722-012-9913-8
[19] Hutcheon, C., Ditt, R.F., Beilstein, M., Comai, L., Schroeder, J., Goldstein, E., Shewmaker, C.K., Nguyen, T., De Rocher, J. and Kiser, J. (2010) Polyploid Genome of Camelina sativa Revealed by Isolation of Fatty Acid Synthesis Genes. BMC Plant Biology, 10, 233.
http://dx.doi.org/10.1186/1471-2229-10-233
[20] Kagale, S., Koh, C., Nixon, J., Bollina, V., Clarke, W.E., Tuteja, R., Spillane, C., Robinson, S.J., Links, M.G., Clarke, C., Higgins, E.E., Huebert, T., Sharpe, A.G. and Parkin, I.A. (2014) The Emerging Biofuel Crop Camelina sativa Retains a Highly Undifferentiated Hexaploid Genome Structure. Nature Communications, 5, Article ID: 3706.
http://dx.doi.org/10.1038/ncomms4706
[21] Plessers, A.G., McGregor, W.G., Carson, R.B. and Nakoneshny, W. (1962) Species Trials with Oilseed Plants: II. Camelina. Canadian Journal of Plant Science, 42, 452-459.
http://dx.doi.org/10.4141/cjps62-073
[22] Bardini, M., Lee, D., Donini, P., Mariani, A., Gianì, S., Toschi, M., Lowe, C. and Breviario, D. (2004) Tubulin-Based Polymorphism (TBP): A New Tool, Based on Functionally Relevant Sequences, to Assess Genetic Diversity in Plant Species. Genome, 47, 281-291.
http://dx.doi.org/10.1139/g03-132
[23] Breviario, D., Vance Baird, W., Sangoi, S., Hilu, K., Blumetti, P. and Gianì, S. (2007) High Polymorphism and Resolution in Targeted Fingerprinting with Combined β-Tubulin Introns. Molecular Breeding, 20, 249-259.
http://dx.doi.org/10.1007/s11032-007-9087-9
[24] Braglia, L., Manca, A., Mastromauro, F. and Breviario, D. (2010) cTBP: A Successful ILP-Based Genotyping Method Targeted to Well Defined Experimental Needs. Diversity, 2, 572-585.
http://dx.doi.org/10.3390/d2040572
[25] Poczai, P., Varga, I., Laos, M., Cseh, A., Bell, N., Valkonen, J.P.T. and Hyvönen, J. (2013). Advances in Plant Gene Targeted and Functional Markers: A Review. Plant Methods, 9, 6.
http://dx.doi.org/10.1186/1746-4811-9-6
[26] Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kuma, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731-2739.
http://dx.doi.org/10.1093/molbev/msr121
[27] Doyle, J.J. and Doyle, J.L. (1987) A Rapid DNA Isolation Procedure for Small Quantities of Fresh Leaf Tissue. Phytochemical Bulletin, 19, 11-15.
[28] Schwarzacher, T. and Heslop-Harrison, J.S. (2000) Practical in Situ Hybridization. BIOS Scientific Publishers, Oxford, 203.
[29] Francis, A. and Warwick, S.I. (2009) The Biology of Canadian Weeds. 142. Camelina alyssum (Mill.) Thell.; C. microcarpa Andrz. ex DC.; C. sativa (L.) Crantz. Canadian Journal of Plant Science, 89, 791-810.
http://dx.doi.org/10.4141/CJPS08185
[30] Jost, W., Baur, A., Nick, P., Reski, R. and Gorr, G. (2004) A Large Plant β-Tubulin Family with Minimal C-Terminal Variation but Differences in Expression. Gene, 340, 151-160.
http://dx.doi.org/10.1016/j.gene.2004.06.009
[31] Oakley, R.V., Wang, Y.S., Ramakrishna, W., Harding, S.A. and Tsai, C.J. (2007) Differential Expansion and Expression of α- and β-Tubulin Gene Families in Populus. Plant Physiology, 145, 961-973.
http://dx.doi.org/10.1104/pp.107.107086
[32] Breviario, D., Gianì, S. and Morello, L. (2013) Multiple Tubulins: Evolutionary Aspects and Biological Implications. Plant Journal, 75, 202-218.
http://dx.doi.org/10.1111/tpj.12243

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.