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Characterization of the Causal Organism of Soft Rot of Tomatoes and Other Vegetables and Evaluation of Its Most Aggressive Isolates

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DOI: 10.4236/ajps.2015.64055    3,203 Downloads   3,916 Views   Citations

ABSTRACT

To isolate the causal organism of soft rot of vegetables, diseased samples of potato, tomato, carrot, chilies, and bell pepper, were analyzed in the lab, using nutrient agar (NA) and/or the enrichment host (Bell pepper) technique. Successful isolations were purified by sub-culturing, identified as Erwinia carotovora subsp. carotovora through biochemical tests and their pathogenicity was confirmed through inoculation on green tomato fruits. The isolates were tested for their aggressiveness to find out the most aggressive one in term of producing maximum soft rot on tomatoes. CRD (completely randomized design), with four replication was used and data were analyzed using LSD (least significant test) test. Among the five isolates evaluated for aggressiveness on tomato fruits, chili isolate was found to be the most aggressive followed by tomato and potato isolates producing 22.3 mm, 7.9 mm, and 7.8 mm diameter soft rot lesions, respectively.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Akbar, A. , Ahmad, M. , Azra, &. , Neelam, &. , Khan, S. and Ahmad, Z. (2015) Characterization of the Causal Organism of Soft Rot of Tomatoes and Other Vegetables and Evaluation of Its Most Aggressive Isolates. American Journal of Plant Sciences, 6, 511-517. doi: 10.4236/ajps.2015.64055.

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