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No Statistic Correlation between Superoxide Dismutase and Peroxidase Activities and Aluminum-Induced Lipid Peroxidation in Maize, Implying Limited Roles of Both Enzymes in Prevention against Aluminum-Induced Lipid Peroxidation

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DOI: 10.4236/ajps.2011.22017    5,505 Downloads   10,395 Views   Citations


Changes of and correlation among root tolerance index (RTI), root Aluminum (Al) content, root/shoot ratio (RSR), root malondialdehyde (MDA) content, and Superoxide dismutase (SOD) and peroxidase (POD) isoforms of maize YQ 7-96 were investigated under Al stress and removal of the stress (RS). Consequently, Al stress led to significant decreases in RTI, RSR, SOD and POD activities, but resulted in significant increase in root MD A and, Al accumulation in the tissues; Root SOD and POD activities did not correlate with Al and MDA contents in roots; The activities of SOD and POD were much lower in roots than in leaves. It can be concluded that (1) Al stress can lead to lipid peroxidation; (2) there is a larger POD family composed of different POD isoforms, some of which are of tissue-specific expression and play different roles in detoxification of Al in maize; (3) for POD isoforms, POD 2 is root-specific. POD 6 and POD 7 are all leaf-specific, POD 5 is not only root-specific but also RS-responsive; (4) high sensitivity of maize to Al is in part associated with much lower activities of both SOD and POD in roots; and (5) more importantly, both SOD and POD are therefore hinted to be not key players in prevention against Al-induced lipid peroxidation.

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L. Wang, J. Zhao, S. Wu, J. Pan, Z. Huang, Z. Wu, X. Fan and Y. Li, "No Statistic Correlation between Superoxide Dismutase and Peroxidase Activities and Aluminum-Induced Lipid Peroxidation in Maize, Implying Limited Roles of Both Enzymes in Prevention against Aluminum-Induced Lipid Peroxidation," American Journal of Plant Sciences, Vol. 2 No. 2, 2011, pp. 156-164. doi: 10.4236/ajps.2011.22017.


[1] L. V. Kochian, “Cellular mechanisms of aluminum toxicity and resistance in plants,” Annual Review of Plant Physiology and Plant Molecular Biology Vol. 46, June 1995, pp. 237-260.
[2] C. F. Meng, P. K. Jiang, Z. H. Cao, Q. F. Xu, and G. M. Zhou, “Study progress on mechanisms of aluminum toxicity and resistance in plants,” Acta Agriculturae Zhejiangensis, Vol. 21, July, 2009, pp. 411-416.
[3] S. R. Devi, Y. Yamamoto and H. Matsumoto. “An intracellular mechanism of aluminum tolerance associated with high antioxidant status in cultured tobacco cells,” Journal of Inorganic Biochemistry, Vol. 97, September 2003, pp. 59-68.
[4] I. Cakmak and W. J. Horst, “Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, peroxidase activities in root tips of soybean (Glycine max L.),” Physiologia Plantarum, Vol. 83, November 1991, pp. 463-468.
[5] B. Meriga, B. K. Reddy, K. R. Rao L. A. Reddy and P. B. Kishor, “Aluminium-induced production of oxygen radicals, lipid peroxidation and DNA damage in seedlings of rice (Oryza sativa),” Journal of Plant Physiology, Vol. 161, January 2004, pp.63-68.
[6] L. Tamás, M. ?imonovi?ová, J. Huttová and I. Mistrík, “Aluminium stimulated hydrogen production of germinating barley seeds,” Environmental and Experimental Botany, Vol. 51, June 2004, pp.281-288.
[7] L. Tamás, S. Budíková, J. Huttová and I. Mistrík, M. Simonovicová and B. Siroká, “Aluminum-induced cell death of barley-root border cells is correlated with peroxidase- and oxalate oxidase-mediated hydrogen peroxide production,” Plant Cell Reports, Vol. 24, Jun 2005, pp. 189-194.
[8] L. Tamás, J. Huttová, I. Mistrík, M. Simonovicová and B. Siroká, “Aluminium-induced drought and oxidative stress in barley roots,” Journal of Plant Physiology, Vol.163, May 2006, pp. 781-784.
[9] T. Mossor-Pietraszewska, “Effect of aluminium on plant growth and metabolism,” Acta Biochimica Poinical, Vol. 48, 2001, pp. 673-686.
[10] R. Chaffai, B. Marzouk and E. El Ferjani,”Aluminum mediates compositional alterations of polar lipid classes in maize seedlings,” Phytochemistry, Vol. 66, August 2005, pp. 1903-1912.
[11] A. Giannakoula, M. Moustakas, P. Mylona, I. Papadakis and T. Yupsanis, “Aluminum tolerance in maize is correlated with increased levels of mineral nutrients, carbohydrates and proline, and decreased levels of lipid peroxidation and Al accumulation,” Journal of Plant Physiology, Vol. 165, 2008, pp. 385-396.
[12] Z. Rengel, “Uptake of aluminium by plant cells,” New Phytologist, Vol. 134, March 1996, pp. 389-406.
[13] P. R. S. Boscolo, M. Menossi and R.A. Jorge, “Aluminum-induced oxidative stress in maize,” Phytochemistry, Vol. 62, January 2003, 181-189.
[14] L. S. Chen, “Physiological responses and tolerance of plant shoot to aluminum toxicity,” Journal of Plant Physiology and Molecular Biology, Vol. 32, April 2006, pp.143-155.
[15] C. Poschenrieder, B. Gunsé, I. Corrales and J. Barceló, “A glance into aluminum toxicity and resistance in plants,” Science of The Total Environment, Vol. 400, August 2008, pp.356-368.
[16] S. Veljovic-Jovanovic, B. Kukavica, B. Stevanovic and F. Navari-Izzo “Senescence- and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica,” Journal of Experimental Botany, Vol. 57, May 2006, pp. 1759-1768.
[17] é. Darkó, H. Ambrus, é. Stefanovits-Bányai,J. Fodor, F. Bakos and B. Barnabás, “Aluminium toxicity, Al tolerance and oxidative stress in an Al-sensitive wheat genotype and in Al-tolerant lines developed by in vitro microspore selection,” Plant Science, Vol.166, March 2004, pp. 583-591.
[18] S. Doncheva, M. Amenós, C. Poschenrieder and J. Barceló, “Root cell patterning: a primary target for aluminium toxicity in maize,” Journal of Experimental Botany, Vol. 56, April 2005, pp. 1213 -1220.
[19] D. R. Hoagland and D. I. Arnon, ”The water-culture method for growing plants without soil,” Circular 347, University of California, College of Agriculture, Berkeley,1938.
[20] J. E. Shaff, B. A. Schultz, E. J. Craft, R. T. Clark and L. V. Kochian, “GEOCHEM-EZ: a chemical speciation program with greater power and flexibility,” Plant and Soil Journal, Vol. 330, April 2010, pp. 207-214.
[21] B. He and J. Liang “Highly sensitive spectrophotometric determination of aluminium in plant and soil water with chromazurol S, cetyltrimethylammonium bromide and alcohol,” Journal of Guangxi Agricultural University, Vol. 35, 1995, pp.151-155.
[22] Z. C. Tang, “Experimental Guide of Modern Plant Physiology,” Beijing. Science Press, 1999, pp. 1-425.
[23] T. Zbigniew and P. Wojciech, “Individual and combined effect of anthracene, cadmium, and chloridazone on growth and activity of SOD izoformes in three Scenedesmus species,” Ecotoxicology and Environmental Safety, Vol. 65, November 2006, pp. 323-331.
[24] R. K. Tewari, P. Kumar and P. N. Sharma, “Morphology and oxidative physiology of sulphur-deficient mulberry plants,” Environmental and Experimental Botany, Vol. 68, May 2010, pp. 301-308.
[25] Y. Pan, L. J. Wu and Z. L. Yu, “Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch),” Plant Growth Regulation, Vol. 49, September 2006, pp. 157-165.
[26] L. Tamás, J. Huttová and I. Mistrík, “Inhibition of Al- induced root elongation and enhancement of Al-induced peroxidase activity in Al-sensitive and Al-resistant barley cultivars are positively correlated,” Plant and Soil, Vol. 250, 2003, pp.193-200.
[27] C. H. Goht and Y. Lee, “Aluminum uptake and aluminum-induced rapid root growth inhibition of rice seedlings,” Journal of Plant Biology, Vol. 42, June 1999, pp. 151-158.
[28] W. J. Horst, N. Schmohl, M. Kollmeier, F. Balu?eka and M. Sivaguru, “Does aluminium affect root growth of maize through interaction with the cell wall-plasma membrane-cytoskeleton continuum?” Plant and Soil, 215, 1999, pp.163-174.
[29] M. Kollmeier, P. Dietrich, C. S. Bauer, W. J. Horst and R. Hedrich, “Aluminum activates a citrate-permeable anion channel in the aluminum-sensitive zone of the maize root apex. A comparison between an aluminum-sensitive and an aluminum-resistant cultivar,” Plant Physiology, Vol. 126, May 2001, pp.397-410.
[30] J. F. Ma, R. Shen, S. Nagao and E. Tanimoto, “Aluminum targets elongating cells by reducing cell wall extensibility in wheat roots,” Plant & Cell Physiology, Vol. 45, May 2004, pp. 583-589.
[31] L. G. Maron, M. Kirst, C. Mao, M. J. Milner, M. Menossi and L.V. Kochian, “Transcriptional profiling of aluminum toxicity and tolerance responses in maize roots,” New Phytologist, Vol. 179, April 2008, pp. 116-128.
[32] J. F. Ma and J. Furukava, “Recent progress in the research of external A1 detoxification in higher plants: a minireview,” Journal of Inorgannic Biochemistry, Vol. 97, 2003, pp. 46-51.
[33] R. Takabatake and T. Shimmen, “Inhibition of electrogenesis by aluminum in characean cells,” Plant & Cell Physiology, Vol. 38, 1997, pp.1264-1271.
[34] A. F. Rangel, I. M. Rao and W. J. Horst, “Intracellular distribution and binding state of aluminum in root apices of two common bean (Phaseolus vulgaris) genotypes in relation to Al toxicity,” Physiologia Plantarum, Vol. 135, February 2009, pp. 162-173.
[35] M. C. Kuo and C. H. Kao, “Aluminum effects on lipid peroxidation and antioxidative enzyme activities in rice leaves,” Biologia Plantarum, Vol. 46, 2003, pp.149-152.
[36] A. Giannakoula, M. Moustakas, T. Syros and T. Yupsanis, “Aluminum stress induces up-regulation of an efficient antioxidant system in the Al-tolerant maize line but not in the Al-sensitive line,” Environmental and Experimental Botany, Vol. 67, January 2010, pp. 487-494.
[37] G. G. Yannarelli, S. M. Gallego and M. L. Tomaro, “Effect of UV-B radiation on the activity and isoforms of enzymes with peroxidase activity in sunflower cotyledons,” Environmental and Experimental Botany, Vol. 56, June 2006, pp.174-181.
[38] J. M. Matés, “Effects of antioxidant enzymes in the molecular control of reactive oxygen species toxicology,” Toxicology, Vol. 153, November 2000, pp. 83-104.
[39] F. C. Lidon and M. da Gra?a Barreiro, “An overview into aluminium toxicity in maize,” Bulgarian Journal of Plant Physiology, Vol. 28, 2002, pp. 96-112.
[40] R. Mittler, “Oxidative stress, antioxidants and stress tolerance,” Trends in Plant Science, Vol.7, September 2002, pp. 405-410.
[41] B. Gunsé, C. Poschenrieder and J. Barceló, “Water transport properties of roots and root cortical cells in proton- and Al-stressed maize varieties,” Plant Physiology, Vol. 113, February 1997, pp. 595-602.
[42] Y. Yamamoto, A. Hachiya and H. Matsumoto, “Oxidative damage to membranes by a combination of aluminum and iron in suspension-cultured tobacco cells,” Plant & Cell Physiology, Vol. 38, 1997, pp. 1333-1339.

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