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Human Nail Clippings as a Source of DNA for Genetic Studies

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DOI: 10.4236/ojepi.2015.51006    3,984 Downloads   4,815 Views   Citations

ABSTRACT

Blood samples have traditionally been used as the main source of DNA for genetic analysis. How-ever, this source can be difficult in terms of collection, transportation, and long-term storage. In this study, we investigated whether human nail clippings could be used as a source of DNA for SNP genotyping, null-allele detection, and whole-genome amplification. From extracted nail DNA, we achieved amplicons up to a length of ~400 bp and >96% concordance for SNP genotyping and 100% concordance for null-allele detection compared to DNA derived from matched blood sam-ples. For whole-genome amplification, OmniPlex performed better than Multiple Displacement Amplification with a success rate of 89.3% and 76.8% for SNP genotyping and null-allele detection, respectively. Concordance was ~98% for both methods. When combined with OmniPlex whole-genome amplification, human nail clippings could potentially be used as an alternative to whole blood as a less invasive and more convenient source of DNA for genotyping studies.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Truong, L. , Park, H. , Chang, S. , Ziogas, A. , Neuhausen, S. , Wang, S. , Bernstein, L. and Anton-Culver, H. (2015) Human Nail Clippings as a Source of DNA for Genetic Studies. Open Journal of Epidemiology, 5, 41-50. doi: 10.4236/ojepi.2015.51006.

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