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Cryopreservation of Seeds and Embryos of Jatropha curcas L.

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DOI: 10.4236/ajps.2015.61020    3,690 Downloads   4,225 Views   Citations

ABSTRACT

Jatropha curcas is a species with a variety of uses. It is grown primarily for oil for biodiesel, but also has agronomic and medicinal applications. Two methods were evaluated for cryopreservation of seeds and zygotic embryos of J. curcas: desiccation followed by rapid immersion of seeds and embryos in liquid nitrogen (LN, -196°C), and vitrification of zygotic embryos. Prior to cryo-preservation, seeds were manually scarified and the moisture content (MC) of seeds and embryos was determined. Explants were disinfected after cryopreservation. Seed germination after LN exposure was 100%. Plantlet development was better in sand substrate than that in vitro. Survival of zygotic embryos after cryopreservation was also 100%, without significant differences between treatments. Optimal development (100%) and plantlet length (51.77 mm) were observed with embryos dried for 60 min to 9.4% MC under laminar flow prior to cryopreservation. Zygotic embryos subjected to the vitrification procedure did not withstand LN exposure. Survival data for non-cryopreserved embryos after each step of the vitrification procedure provided information about embryo tolerance to cryoprotectants.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Prada, J. , Aguilar, M. , Abdelnour-Esquivel, A. and Engelmann, F. (2015) Cryopreservation of Seeds and Embryos of Jatropha curcas L.. American Journal of Plant Sciences, 6, 172-180. doi: 10.4236/ajps.2015.61020.

References

[1] Machado, R. and Suárez, J. (2009) Performance of Three Provenances of Jatropha curcas in the Germoplasm Bank of the EEPF “Indio Hatuey”. Pastos y Forrajes, 32, 29-37.
[2] CATIE (Centro Agronómico Tropical de Investigación y Enseñanza) (2003) Arboles de Centroamérica: Un manual para extensionistas. Cordero, J. y Boshier, D. Turrialba, CR, 1079 p.
[3] Jiménez, R. and Martínez, G. (1994) Redescripción de Jatrophaandrieuxii Muell. Arg. (Euphorbiaceae), una especie endémica del sur de México. Acta Botánica Mexicana, 26, 27-32.
[4] Martínez, G., Jiménez, R., Cruz, R., Juárez, A., Garcia, R., Cervantes, A. and Mejía, R. (2002) Los géneros de la familia Euphorbiaceae en México. Anales del Instituto de Biología, UNAM. Ser Bot: UNAM Serie Botanica, 73, 155-281.
[5] Kone-Bamba, D., Pelissier, Y., Ozouko, Z. and Ouao, D. (1987) Etude de l’activité hémostatique de quinze plantes médicinales de la “Pharmacopée Traditionnelle Ivoirienne”. Plantes Médicinales et Phytothérapie, 21, 121-130.
[6] Heller, J. (1996) Physic Nut. Jatropha curcas L. Promoting the Conservation and Use of Underutilized and Neglected Crops. 1. Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute, Rome, 66 p.
[7] Grainge, M. and Ahmed, S. (1988) Handboook of Plants with Pest-Control Properties. John Wiley & Sons, Hoboken, 470 p.
[8] Mukherjee, P., Varhney, A., Johnson, T.S. and Jha, T.B. (2011) Jatropha curcas: A Review on Biotechnological Status and Challenges. Plant Biotechnology Reports, 5, 197-215.
[9] Deng, Z.J., Xiang, Z.Y., Cheng, H.Y., Li, Y.J. and Song, S.Q. (2008) Preliminary Study on Development, Germination and Desiccation Tolerance of Jatropha curcas (Euphorbiaceae). Acta Botanica Yunnanica, 30, 355-359.
[10] Joker, D. and Jepsen, J. (2003) Jatropha curcas L. Seed Leaflet, No 83 August Danida Forest Seed Centre. Demark, 81-108.
[11] GonzalezArnao, M.T. and Engelmann, F. (2013) Introducción a la conservación ex situ de los recursos genéticos vegetables. In: GonzalezArnao, M.T. and Engelmann, F., Eds., Crioconservación de plantas en América Latina y el Caribe, Editorial Instituto Interamericano de Cooperación para la Agricultura, San José, 39-48.
[12] Engelmann, F. (1991) In Vitro Conservation of Tropical Plant Germplasm—A Review. Euphytica, 57, 227-243. http://dx.doi.org/10.1007/BF00039669
[13] Reed, B.M. (2008) Plant Cryopreservation: A Practical Guide. Springer, New York.
http://dx.doi.org/10.1007/978-0-387-72276-4
[14] Hine-Gómez, A. and Abdelnour-Esquivel, A. (2013) Establecimiento in vitro de arándano (Vaccinium corymbosum L). Tecnología en Marcha, 26, 64-71.
[15] Engelmann, F. (2011) Use of Biotechnologies for the Conservation of Plant Biodiversity. In Vitro Cellular & Developmental Biology—Plant, 47, 5-16. http://dx.doi.org/10.1007/s11627-010-9327-2
[16] International Seed Testing Association (ISTA) (2008) International Ruler For Seed Testing: The Germination Test. Seed Science and Technology, p. 7.
[17] Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497.
http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
[18] Matsumoto, T., Sakai, A. and Yamada, K. (1994) Cryopreservation of in Vitro Grown Apical Meristems of Wasabi (Wasabia japonica) by Vitrification and Subsequent High Plant Regeneration. Plant Cell Reports, 13, 442-446. http://dx.doi.org/10.1007/BF00231963
[19] Sakai, A., Kobayashi, S. and Oiyama, I. (1990) Cryopreservation of Nucellar Cells of Navel Orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by Vitrification. Plant Cell Reports, 9, 30-33.
[20] Di Rienzo, J.A., et al. (2008) Infostat: Manual del usuario versión 2008. Coordinado por Di Rienzo, J.A.-1ed.-Brujas, Cordoba, ISBN 98-987-591-100-9.
[21] Silva, R., Camillo, J. and Pereira, S. (2012) A Method for Seedling Recovery in Jatropha curcas after Cryogenic Exposure of the Seeds. Revista de Biologia Tropical, 60, 473-482.
[22] Toral, O., Iglesias, J., Oca, S., Sotolongo, J., Garcia, S. and Torsti, M. (2008) Jatrpha curcas L., a Tree Species with Energetic Potential in Cuba. Pastos y Forrajes, 31, 191-207.
[23] Verma, K. and Gaur, A. (2009) Jatropha curcas L.: Substitute for Conservation Energy. World Journal of Agricultural Sciences, 5, 552-556.
[24] Abdelnour-Esquivel, A., Villalobos, V. and Engelmann, F. (1992) Cryopreservation of Zygotic Embryos of Coffea spp. Cryoletters, 13, 197-302.
[25] Normah, N., Choo, K., Yap, V. and Zeti, M. (2011) In Vitro Conservation of Malaysian Biodiversity-Archievements, Challenges and Future Directions. In Vitro Cellular & Developmental Biology—Plant, 47, 26-36. http://dx.doi.org/10.1007/s11627-010-9306-7
[26] Abdelnour-Esquivel, A. and Aguilar, M.E. (2013) Crioconservación de germoplasma vegetal en Costa Rica. In: GonzalezArnao, M.T. and Engelmann, F., Eds., Crioconservación de plantas en America Latina y el Caribe, Editorial Instituto Interamericano de Cooperación para la Agricultura, San José, 113-126.
[27] Scocchi, A., Dieringer, E., Mroginski, E. and Mroginski, L. (2004) Conservación de semillas de cedro australiano (Toona ciliata). Plant Genetic Resources Newsletter, 137, 1-4.
[28] Scocchi, A.M. and Rey, H.Y. (2004) Conservación de germoplasma in vitro. In: Echenique, V., Rubinstein, C. and Mroginski, L., Eds., Biotecnología y mejoramiento vegetal, Ediciones INTA, Buenos Aires, 179-185.
[29] Abdelnour-Esquivel, A., Rojas, G. and Alfaro, U. (2007) Estudios preliminares para la crioconservación de especies forestales arbóreas. Tecnología en Marcha, 20, 98-103.
[30] Aguilar, M.E. and Abdelnour-Esquivel, A. (2010) Desarrollo de modelos para la crioconservación de semillas y material clonal de especies forestales de Costa Rica en peligro de extinción y seleccionadas en programas de mejoramiento genético. Boletin de Ciencia y Tecnología No. 99, San José, CONICIT. http://163.178.205.6/boletin/boletin99/PalabrasMariaElenaAguilar.html
[31] Meryman, H.T., Williams, R.J. and Douglas, S.J. (1977) Freezing Injury from Solution Effects and Its Prevention by Natural or Artificial Cryoprotection. Cryobiology, 14, 287-302.
http://dx.doi.org/10.1016/0011-2240(77)90177-8
[32] Goldfarb, M., Duarte, M. and Mata, M. (2010) Armazenamento criogênico de sementes de pinhão manso (Jatropha curcas L.) Euphorbiaceae. Revista Biotemas, 23, 27-33.

  
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