Share This Article:

Characterization of an Early Berry Development Grapevine Somatic Variant (Vitis labrusca L. cv. Isabel Precoce)

Abstract Full-Text HTML XML Download Download as PDF (Size:3709KB) PP. 3848-3865
DOI: 10.4236/ajps.2014.526403    3,649 Downloads   4,230 Views   Citations

ABSTRACT

Over the past 10 years significant advances have been made towards the description of genetics and molecular mechanisms controlling grapevine berry growth. Regardless of this, many aspects of early fruit morphogenesis and its development control remain to be elucidated. In an attempt to understand gene expression patterns associated with the berry growth development, the contrasting phenotype between the cv. Isabel (Vitis labrusca L.) and its early berry development mutant “Isabel Precoce” has been explored by a candidate gene approach. “Isabel Precoce” (Vitis labrusca L.) was confirmed as an EDV (Essentially Derived Variety) of Isabel, with a 30-35-day reduction in the berry growth phase when compared to the wild type and thus, it constitutes an informative model to investigate many aspects of fruit growth and development. Phenotypic analysis showed that “Isabel Precoce” develops fruits that are smaller in diameter and volume despite of following similar development kinetics. The expression of many genes associated with plant growth and development (MIKCC-type MADS box genes), sugar transport and with the control of flavonoid biosynthetic pathway have been evaluated. The majority of the genes presented a remarkably similar transcription profile. However, a higher induction of transcript accumulation for some genes has been observed in the “Isabel Precoce” genetic background.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Passaia, G. , Margis-Pinheiro, M. , Fialho, F. , Sbeghen, F. , Porto, D. and Revers, L. (2014) Characterization of an Early Berry Development Grapevine Somatic Variant (Vitis labrusca L. cv. Isabel Precoce). American Journal of Plant Sciences, 5, 3848-3865. doi: 10.4236/ajps.2014.526403.

References

[1] Fernandez, L., Torregrosa, L., Terrier, N., Sreekantan, L., Grimplet, J., Davies, C., et al. (2007) Identification of Genes Associated with Flesh Morphogenesis during Grapevine Fruit Development. Plant Molecular Biology, 63, 307-323. http://dx.doi.org/10.1007/s11103-006-9090-2
[2] Jaillon, O., Aury, J.M., Noel, B., Policriti, A., Clepet, C., Casagrande, A., et al. (2007) The Grapevine Genome Sequence Suggests Ancestral Hexaploidization in Major Angiosperm Phyla. Nature, 449, 463-467. http://dx.doi.org/10.1038/nature06148
[3] Burger, A.L.B. and Botha, F.C.B. (2004) Ripening-Related Gene Expression during Fruit Ripening in Vitis vinifera L. cv. Cabernet Sauvignon and Clairette Blanche. Vitis, 43, 59-64.
[4] Goes, F., Iandolino, A., Al-kayal, F., Bohlmann, M.C., Cushman, M.A., Lim, H., et al. (2005) Characterizing the Grape Transcriptome Analysis of Expressed Sequence Tags from Multiple Vitis Species and Development of a Compendium of Gene Expression during Berry Development. Plant Physiology, 139, 574-597. http://dx.doi.org/10.1104/pp.105.065748
[5] Terrier, N., Glissant, D., Grimplet, J., Barrieu, F., Abbal, P., Couture, C., et al. (2005) Isogene Specific Oligo Arrays Reveal Multifaceted Changes in Gene Expression during Grape Berry (Vitis vinifera L.) Development. Planta, 222, 832-847. http://dx.doi.org/10.1007/s00425-005-0017-y
[6] Waters, D.L.E., Holton, T. A., Ablett, E.M., Slade, L. and Henry, R.J. (2006) The Ripening Wine Grape Berry Skin Transcriptome. Plant Science, 171, 132-138.
http://dx.doi.org/10.1016/j.plantsci.2006.03.002
[7] Zamboni, A., Minoia, L., Ferrarini, A., Tornielli, G.B., Zago, E., Delledonne, M., et al. (2008) Molecular Analysis of Post-Harvest Withering in Grape by AFLP Transcriptional Profiling. Journal of Experimental Botany, 59, 4145-4159. http://dx.doi.org/10.1093/jxb/ern256
[8] Zamboni, A., Di Carli, M., Guzzo, F., Stocchero M., Zenoni, S., Ferrarini, A., et al. (2010) Identification of Putative Stage-Specific Grapevine Berry Biomarkers and Omics Data Integration into Networks. Plant Physiology, 154, 1439-1459. http://dx.doi.org/10.1104/pp.110.160275
[9] Zenoni, S., Ferrarini, A., Giacomelli, E., Xumerle, L., Fasoli, M., Malerba, G., et al. (2010) Characterization of Transcriptional Complexity during Berry Development in Vitis vinifera Using RNA-Seq. Plant Physiology, 152, 1787-1795. http://dx.doi.org/10.1104/pp.109.149716
[10] Lijavetzky, D., Carbonell-Bejerano, P., Grimplet, J., Bravo, G., Flores, P., Fenoll, J., et al. (2012) Berry Flesh and Skin Ripening Features in Vitis vinifera as Assessed by Transcriptional Profiling. PLoS One, 7, e39547. http://dx.doi.org/10.1371/journal.pone.0039547
[11] Tornielli, P.M., Zamboni, A., Zenoni, S. and Delledonne, M. (2012) Transcriptomics and Metabolomics for the Analysis of Grape Berry Development. In: Geros, S.D.H. and Chavez, M.M., Eds., The Biochemistry of the Grape Berry, Bentham Science Publishers, Sharjan, 218-240.
[12] Fasoli, M., Dal Santo, S., Zenoni, S., Tornielli, G.B., Farina, L., Zamboni, A., et al. (2012) The Grapevine Expression Atlas Reveals a Deep Transcriptome Shift Driving the Entire Plant into a Maturation Program. Plant Cell, 24, 3489-3505. http://dx.doi.org/10.1105/tpc.112.100230
[13] Boss, P.K., Sensi, E., Hua, C., Davies, C. and Thomas, M.R. (2002) Cloning and Characterisation of Grape Vine (Vitis vinifera L.) MADS-Box Genes Expressed during Inflorescence and Berry Development. Plant Science, 162, 887-895. http://dx.doi.org/10.1016/S0168-9452(02)00034-1
[14] Boss, P.K., Vivier, M., Matsumoto, S., Dry, I.B. and Thomas, M.R. (2001) A cDNA from Grapevine (Vitis vinifera L.), Which Shows Homology to AGAMOUS and SHATTERPROOF, Is Not Only Expressed in Flowers but Also throughout Berry Development. Plant Molecular Biology, 45, 541-553. http://dx.doi.org/10.1023/A:1010634132156
[15] Carmona, M.J., Cubas, P. and Martínez-Zapater, J.M. (2002) VFL, the Grapevine FLORICAULA/LEAFY Ortholog Is Expressed in Meristematic Regions Independently of Their Fate. Plant Physiology, 130, 69-77. http://dx.doi.org/10.1104/pp.002428
[16] Chatelet, P., Laucou, V., Fernandez, L., Sreekantan, L., Lacombe, T., Martinez-Zapater, J.M., et al. (2007) Characterization of Vitis vinifera L. Somatic Variants Exhibiting Abnormal Flower Development Patterns. Journal of Experimental Botany, 58, 4107-4118. http://dx.doi.org/10.1093/jxb/erm269
[17] Fernandez, L., Romieu, C., Moing, A., Bouquet, A., Maucourt, M., Thomas, M.R., et al. (2006) The Grapevine Fleshless Berry Mutation. A Unique Genotype to Investigate Differences between Fleshy and Nonfleshy Fruit. Plant Physiology, 140, 537-547. http://dx.doi.org/10.1104/pp.105.067488
[18] Boss, P.K. and Thomas, M.R. (2002) Association of Dwarfism and Floral Induction with a Grape “Green Revolution” Mutation. Nature, 416, 847-850. http://dx.doi.org/10.1038/416847a
[19] Kobayashi, S., Goto-yamamoto, N. and Hirochika, H. (2004) Retrotransposon-Induced Mutations in Grape Skin Color. Science, 304, 982. http://dx.doi.org/10.1126/science.1095011
[20] Ageorges, A., Fernandez, L., Vialet, S., Merdinoglu, D., Terrier, N. and Romieu, C. (2006) Four Specific Isogenes of the Anthocyanin Metabolic Pathway Are Systematically Co-Expressed with the Red Color of Grape Berries. Plant Science, 170, 372-383. http://dx.doi.org/10.1016/j.plantsci.2005.09.007
[21] Fernandez, L., Chaïb, J., Martinez-Zapater, J.M., Thomas, M.R. and Torregrosa, L. (2013) Mis-Expression of a PISTILLATA-Like MADS Box Gene Prevents Fruit Development in Grapevine. Plant Journal, 73, 918-928. http://dx.doi.org/10.1111/tpj.12083
[22] Fernandez, L., Torregrosa, L., Segura, V., Bouquet, A. and Martinez-Zapater, J.M. (2010) Transposon-Induced Gene Activation as a Mechanism Generating Cluster Shape Somatic Variation in Grapevine. Plant Journal, 61, 545-557. http://dx.doi.org/10.1111/j.1365-313X.2009.04090.x
[23] Torregrosa, L., Fernandez, L., Bouquet, A., Boursiquot, J.M., Pelsy, F. and Martínez-Zapater, J.M. (2011) Origins and Consequences of Somatic Variation in Grapevine. In: Kole, C., Ed., Genetics, Genomics, and Breeding of Grapes, Science Publishers, Enfield, 68-92.
[24] Meier, U. (2001) Growth Stages of Mono and Dicotyledonous Plants. BBCH Monograph, Federal Biological Research Centre for Agriculture and Forestry, Bonn.
[25] Fialho, F. (1999) Interpretação da Curva de Crescimento de Gompertz. Embrapa Suínos e Aves. Comunicado técnico, Concórdia, 237.
[26] Gompertz, B. (1825) On the Nature of the Function Expressive of the Law of Human Mortality, and on a New Mode of Determining the Value of Life Contigencies. Philosophical Transactions of the Royal Society of London, 115, 513-583. http://dx.doi.org/10.1098/rstl.1825.0026
[27] Windsor, C.P. (1932) The Gompertz Curve as a Growth Curve. Proceedings of the National Academy of Sciences of the United States of America, 18, 1-8. http://dx.doi.org/10.1073/pnas.18.1.1
[28] Lodhi, M.A., Ye, G.N., Weeden, N.F. and Reisch, B.I. (1994) A Simple and Efficient Method for DNA Extraction from Grapevine Cultivars and Vitis Species. Plant Molecular Biology Report, 12, 6-13. http://dx.doi.org/10.1007/BF02668658
[29] Lefort, F. and Douglas, G.C. (1999) An Efficient Micro-Method of DNA Isolation from Mature Leaves of Four Hardwood Tree Species Acer, Fraxinus, Prumus and Quercus. Annals of Forest Sciences, 56, 259-263. http://dx.doi.org/10.1051/forest:19990308
[30] This, P., Jung, A., Boccacci, P., Borrego, J., Botta, R., Costantini, L., et al. (2004) Development of a Standard Set of Microsatellite Reference Alleles for Identification of Grape Cultivars. Theoretical and Applied Genetics, 109, 1448- 1458. http://dx.doi.org/10.1007/s00122-004-1760-3
[31] Paetkau, D., Calvert, W., Stirling, I. and Strobeck, C. (1995) Microsatellite Analysis of Population Structure in Canadian Polar Bears. Molecular Ecology, 4, 347-354.
http://dx.doi.org/10.1111/j.1365-294X.1995.tb00227.x
[32] Wagner, H.W. and Sefc, K.M. (1999) Identity 1.0. University of Agricultural Sciences, Vienna.
[33] Reid, K.E., Olsson, N., Schlosser, J., Peng, F. and Lund, S.T. (2006) An Optimized Grapevine RNA Isolation Procedure and Statistical Determination of Reference Genes for Real-Time RT-PCR during Berry Development. BMC Plant Biology, 6, 27. http://dx.doi.org/10.1186/1471-2229-6-27
[34] Livak, K.J. and Schmittgen, T.D. (2001) Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the Method. Methods, 25, 402-408. http://dx.doi.org/10.1006/meth.2001.1262
[35] Sturn, A. and Quackenbush, J. (2002) Genesis: Cluster Analysis of Microarray Data. Bioinformatics, 18, 207-208. http://dx.doi.org/10.1093/bioinformatics/18.1.207
[36] Ramakers, C., Ruijter, J.M., Deprez, R.H.L. and Moorman, A.F. (2003) Assumption-Free Analysis of Quantitative Real-Time Polymerase Chain Reaction (PCR) Data. Neurosciense Letters, 339, 62-66. http://dx.doi.org/10.1016/S0304-3940(02)01423-4
[37] Ruijter, J.M., Ramakers, C., Hoogaars, W.M., Karlen, Y., Bakker, O. and van den Hoff, M.J. (2009) Amplification Efficiency: Linking Baseline and Bias in the Analysis of Quantitative PCR Data. Nucleic Acids Research, 37, e45. http://dx.doi.org/10.1093/nar/gkp045
[38] Camargo, U.A. (2004) Isabel Precoce: Alternativa Para a Vitivinicultura Brasileira. Comunicado técnico 54, Embrapa Uva e Vinho, Bento Gonçalves.
[39] Aradhya, M.K., Dangl, G.S., Prins, B.H., Boursiquot, J.M., Walker, M.A., Meredith, C.P., et al. (2003) Genetic Structure and Differentiation in Cultivated Grape, Vitis vinifera L. Genetical Research, 81, 179-192. http://dx.doi.org/10.1017/S0016672303006177
[40] Franks, T., Botta, R. and Thomas, R. (2002) Chimerism in Grapevines: Implications for Cultivar Identity, Ancestry and Genetic Improvement. Theoretical and Applied Genetics, 104, 192-199. http://dx.doi.org/10.1007/s001220100683
[41] Riaz, S., Garrison, K.E., Dangl, G.S., Roi, G. and Meredith, C.P. (2002) Genetic Divergence and Chimerism within Ancient Asexually Propagated Winegrape Cultivars. Journal of the American Society for Horticultural Science, 127, 508-514.
[42] Vignani, R., Bowers, J.E. and Meredith, C.P. (1996) Microsatellite DNA Polymorphism Analysis of Clones of Vitis vinifera Sangiovese. Sciencia Horticulturae, 65, 163-169.
http://dx.doi.org/10.1016/0304-4238(95)00865-9
[43] Ibánez, J. and Vélez, M. (2007) A Microsatellite Based System for the Protection of Grapevine Varieties. Bulletin De L'OIV, 914-916.
[44] Coombe, B.G. and Hale, C.R. (1973) The Hormone Content of Ripening Grape Berries and the Effects of Growth Substance Treatments. Plant Physiology, 51, 629-634.
http://dx.doi.org/10.1104/pp.51.4.629
[45] Symons, G.M., Davies, C., Shavrukov, Y., Dry, I.B., Reid, J.B. and Thomas, M.R. (2006) Grapes on Steroids. Brassinosteroids Are Involved in Grape Berry Ripening. Plant Physiology, 140, 150-158. http://dx.doi.org/10.1104/pp.105.070706
[46] Grimplet, J., Deluc, L.G., Tillett, R.L., Wheatley, M.D., Schlauch, K.A., Cramer, G.R., et al. (2007) Tissue-Specific mRNA Expression Profiling in Grape Berry Tissues. BMC Genomics, 8, 187.
http://dx.doi.org/10.1186/1471-2164-8-187
[47] Fortes, A.M., Agudelo-Romero, P., Silva, M.S., Ali, K., Sousa, L., Maltese, F., et al. (2011) Transcript and Metabolite Analysis in Trincadeira Cultivar Reveals Novel Information Regarding the Dynamics of Grape Ripening. BMC Plant Biology, 11, 149. http://dx.doi.org/10.1186/1471-2229-11-149
[48] Deluc, L.G., Quilici, D.R., Decendit, A., Grimplet, J., Wheatley, M.D., Schlauch, K.A., et al. (2009) Water Deficit Alters Differentially Metabolic Pathways Affecting Important Flavor and Quality Traits in Grape Berries of Cabernet Sauvignon and Chardonnay. BMC Genomics, 10, 212.
http://dx.doi.org/10.1186/1471-2164-10-212
[49] Castellarin, S.D., Pfeiffer, A., Sivilotti, P., Degan, M., Peterlunger, E. and Di Gaspero, G. (2007) Transcriptional Regulation of Anthocyanin Biosynthesis in Ripening Fruits of Grapevine under Seasonal Water Deficit. Plant, Cell & Environment, 30, 1381-1399.
http://dx.doi.org/10.1111/j.1365-3040.2007.01716.x
[50] Díaz-Riquelme, J., Lijavetzky, D., Martínez-Zapater, J.M. and Carmona, M.J. (2009) Genome-Wide Analysis of MIKCC-Type MADS Box Genes in Grapevine. Plant Physiology, 149, 354-369. http://dx.doi.org/10.1104/pp.108.131052
[51] Rounsley, S.D., Ditta, G.S. and Yanofsky, M.F. (1995) Diverse Roles for MADS Box Genes in Arabidopsis Development. Plant Cell, 7, 1259-1269.
[52] Colombo, L. (1997) Downregulation of Ovule-Specific MADS Box Genes from Petunia Results in Maternally Controlled Defects in Seed Development. Plant Cell Online, 9, 703-715.
http://dx.doi.org/10.1105/tpc.9.5.703
[53] Mejía, N., Soto, B., Guerrero, M., Casanueva, X., Houel, C., Miccono, M.D.L.á., et al. (2011) Molecular, Genetic and Transcriptional Evidence for a Role of VvAGL11 Stenospermocarpic Seedlessness in Grapevine. BMC Plant Biology, 11, 57. http://dx.doi.org/10.1186/1471-2229-11-57
[54] Conde, C., Silva, P., Fontes, N., Dias, A.C.P., Tavares, R.M., Sousa, M.J., et al. (2007) Biochemical Changes throughout Grape Berry Development and Fruit and Wine Quality. Food, 1, 1-22.
[55] Bogs, J., Jaffé, F.W., Takos, A.M., Walker, A.R. and Robinson, S.P. (2007) The Grapevine Transcription Factor VvMYBPA1 Regulates Proanthocyanidin Synthesis during Fruit Development. Plant Physiology, 143, 1347-1361. http://dx.doi.org/10.1104/pp.106.093203
[56] Çakir, B., Agasse, A., Gaillard, C., Saumonneau, A., Delrot, S. and Atanassova, R. (2003) A Grape ASR Protein Involved in Sugar and Abscisic Acid Signaling. Plant Cell, 15, 2165-2180.
http://dx.doi.org/10.1105/tpc.013854
[57] Boss, P.K., Vivier, M., Matsumoto, S., Dry, I.B. and Thomas, M.R. (2001) A cDNA from Grapevine (Vitis vinifera L.), Which Shows Homology to AGAMOUS and SHATTERPROOF, Is Not Only Expressed in Flowers but Also throughout Berry Development. Plant Molecular Biology, 45, 541-553. http://dx.doi.org/10.1023/A:1010634132156
[58] Skreekantan, L. and Thomas, M.R. (2006) VvFT and VvMADS8 the Grapevine Homologues of the Floral Integrators FT and SOC1, Have Unique Expression Patterns in Grapevine and Hasten Flowering in Arabidopsis. Functional Plant Biology, 33, 1129-1139. http://dx.doi.org/10.1071/FP06144
[59] Tesniere, C., Davies, C., Skreekantan, L., Bogs, J. and Thomas, M.R. (2006) Analysis of the Transcript Levels of VvAdh1, VvAdh2 and VvGrip4, Three Genes Highly Expressed during Vitis vinifera L. Berry Development. Vitis, 45, 75-79.
[60] Jeong, S., Goto-Yamamoto, N., Kobayashi, S. and Esaka, M. (2004) Effects of Plant Hormones and Shading on the Accumulation of Anthocyanins and the Expression of Anthocyanin Biosynthetic Genes in Grape Berry Skins. Plant Science, 167, 247-252. http://dx.doi.org/10.1016/j.plantsci.2004.03.021

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.