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Agro-Industrial Waste Materials as Substrates for the Production of Poly(3-Hydroxybutyric Acid)

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DOI: 10.4236/jbnb.2014.54027    3,907 Downloads   5,012 Views   Citations

ABSTRACT

Accumulation of recalcitrant plastics in the environment has become a world-wide problem in today’s societies. Rapid depletion of natural resources for synthetic plastics along with environmental concerns has directed research towards finding alternatives to petroleum-based polymers. Poly(3-hydroxybutyric acid) P(3HB), as one of these alternatives, have attracted much attention in recent years due to their varied mechanical properties, biocompatibility and biodegradability. The aim of this study was to identify an agro-industrial waste resource economically suitable for large-scale production of P(3HB), to optimize the production using Response Surface Methodology in small-scale and subsequently, to test the production in a continuously stirred tank reactor. Among a range of agro-industrial waste, orange peel was selected as the most suitable for P(3HB) production. P(3HB) concentration of 1.24 g P(3HB)/L culture broth with 41% P(3HB)/dcw yield was obtained using orange peel as the sole carbon source in optimized medium with a modified strain of Bacillus subtilis (B. subtilis OK2).

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Sukan, A. , Roy, I. and Keshavarz, T. (2014) Agro-Industrial Waste Materials as Substrates for the Production of Poly(3-Hydroxybutyric Acid). Journal of Biomaterials and Nanobiotechnology, 5, 229-240. doi: 10.4236/jbnb.2014.54027.

References

[1] Khanna, S. and Srivastava, A.K. (2005) Recent Advances in Microbial Polyhydroxyalkanoates. Process Biochemistry, 40, 607-619. http://dx.doi.org/10.1016/j.procbio.2004.01.053
[2] (2010) Eco-Cycle Environmental Facts and Figures to Inspire Action toward Zero Waste.
http://www.ecocycle.org/files/pdfs/Eco-CycleEnvironmentalFacts.pdf
[3] Francis, L. (2011) Biosynthesis of Polyhydroxyalkanoates and Their Medical Applications. University of Westminster, London.
[4] Castilho, L.R., Mitchell, D.A. and Freire, D.M.G. (2009) Production of Polyhydroxyalkanoates (PHAs) from Waste Materials and By-Products by Submerged and Solid-State Fermentation. Bioresource Technology, 100, 5996-6009.
http://dx.doi.org/10.1016/j.biortech.2009.03.088
[5] Philip, S., Keshavarz, T. and Roy, I. (2007) Polyhydroxyalkanoates: Biodegradable Polymers with a Range of Applications. Journal of Chemical Technology and Biotechnology, 82, 233-247.
http://dx.doi.org/10.1002/jctb.1667
[6] Albuquerque, M., Eiroa, M., Torres, C., Nunes, B. and Reis, M. (2007) Strategies for the Development of a Side Stream Process for Polyhydroxyalkanoate (PHA) Production from Sugar Cane Molasses. Journal of Biotechnology, 130, 411-421. http://dx.doi.org/10.1016/j.jbiotec.2007.05.011
[7] Keshavarz, T. and Roy, I. (2010) Polyhydroxyalkanoates: Bioplastics with a Green Agenda. Current Opinion in Microbiology, 13, 321-326. http://dx.doi.org/10.1016/j.mib.2010.02.006
[8] Valappil, S. P., Rai, R., Bucke, C. and Roy, I. (2008) Polyhydroxyalkanoate Biosynthesis in Bacillus cereus SPV under Varied Limiting Conditions and an Insight into the Biosynthetic Genes Involved. Journal of Applied Microbiology, 104, 1624-1635. http://dx.doi.org/10.1111/j.1365-2672.2007.03678.x
[9] Wang, Y., Hua, F., Tsang, Y., Chan, S., Sin, S., Chua, H., Yu, P. and Ren, N. (2007) Synthesis of PHAs from Waster under Various C:N Ratios. Bioresource Technology, 98, 1690-1693.
http://dx.doi.org/10.1016/j.biortech.2006.05.039
[10] Johnson, K., Kleerebezem, R. and van Loosdrecht, M. (2010) Influence of the C/N Ratio on the perf5ormance of polyhydroxybutyrate (P(3HB)) Producing Sequencing Batch Reactors at Short SRTs. Water Research, 44, 52141-2152.
http://dx.doi.org/10.1016/j.watres.2009.12.031
[11] Hong, K., Chen, G.Q., Yu, P.H.F., Zhang, G., Liu, Y. and Chua, H. (2000) Effect of C:N Molar Ratio on Monomer Composition of Polyhdroxyalk Anoates Produced by Pseudomonas mendocina 0806 and Pseudomonas pseudoalkaligenus YS1. Applied Biochemistry and Biotechnology, 84, 971-980. http://dx.doi.org/10.1385/ABAB:84-86:1-9:971
[12] Hartmann, R., Hany, R., Geiger, T., Egli, T., Witholt, B. and Zinn, M. (2004) Tailored Biosynthesis of Olefinic Medium-Chain-Length Poly [(R)-3-hydroxyalkanoates] in Pseudomonas putida GPo1 with Improved Thermal Properties. Macromolecules, 37, 6780-6785. http://dx.doi.org/10.1021/ma040035+
[13] Anderson, A.J. and Dawes, E.A. (1990) Occurrence, Metabolism, Metabolic Role and Industrial Uses of Bacterial Polyhydroxyalk Anoates. Microbiological Reviews, 54, 450-472.
[14] Wolf, O., Crank, M., Patel, M., Marscheider-Weidemann, F., Schleich, J., Hüsing, B. and Angerer, G. (2005) Techno-Economic Feasibility of Large-Scale Production of Bio-Based Polymers in Europe. European Communities.
[15] Akaraonye, E., Keshavarz, T. and Roy, I. (2010) Production of Polyhydroxyalkanoates: The Future Green Materials of Choice. Journal of Chemical Technology and Biotechnology, 85, 732-743. http://dx.doi.org/10.1002/jctb.2392
[16] Albuquerque, M., Torres, C. and Reis, M. (2010) Polyhydroxyalkanoate (PHA) Production by a Mixed Microbial Culture Using Sugar Molasses: Effect of the Influent Substrate Concentration on Culture Selection. Water Research, 44, 3419-3433. http://dx.doi.org/10.1016/j.watres.2010.03.021
[17] Omar, S., Rayes, A., Eqaab, A., VOß, I. and Steinbüchel, A. (2001) Optimization of Cell Growth and P3HB Accumulation on Date Syrup by a Bacillus megaterium Strain. Biotechnology Letters, 23, 1119-1123.
http://dx.doi.org/10.1023/A:1010559800535
[18] Solaiman, D.K.Y., Ashby, R.D., Hotchkiss, A.T. and Foglia, T.A. (2006) Biosynthesis of Medium-Chain-Length Poly (hydroxyalkanoates) from Soy Molasses. Biotechnology Letters, 28, 157-162.
http://dx.doi.org/10.1007/s10529-005-5329-2
[19] Nikel, P.I., De Almeida, A., Melillo, E.C., Galvagno, M.A. and Pettinari, M.J. (2006) New Recombinant Escherichia Coli Strain Tailored for the Production of Poly (3-hydroxybutyrate) from Agroindustrial By-Products. Applied and Environmental Microbiology, 72, 3949-3954.
http://dx.doi.org/10.1128/AEM.00044-06
[20] Koller, M., Bona, R., Chiellini, E., Fernandes, E.G., Horvat, P., Kutschera, C., Hesse, P. and Braunegg, G. (2008) Polyhydroxyalkanoate Production from Whey by Pseudomonas hydrogenovora. Bioresource Technology, 99, 4854-4863.
http://dx.doi.org/10.1016/j.biortech.2007.09.049
[21] Marangoni, C., Furigo, A. and de Aragão, G.M.F. (2002) Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Ralstonia eutropha in Whey and Inverted Sugar with Propionic Acid Feeding. Process Biochemistry, 38, 137-141.
http://dx.doi.org/10.1016/S0032-9592(01)00313-2
[22] Van-Thuoc, D., Quillaguaman, J., Mamo, G. and Mattiasson, B. (2007) Utilization of Agricultural Residues for Poly (3-hydroxybutyrate) Production by Halomonas boliviensis LC1. Journal of Applied Microbiology, 104, 420-428.
[23] Huang, T.Y., Duan, K.J., Huang, S.Y. and Chen, C.W. (2006) Production of Polyhydroxyalkanoates from Inexpensive Extruded Rice Bran and Starch by Haloferax mediterranei. Journal of Industrial Microbiology & Biotechnology, 33, 701-706.
http://dx.doi.org/10.1007/s10295-006-0098-z
[24] Halami, P.M. (2008) Production of Polyhydroxyalkanoate from Starch by the Native Isolate Bacillus cereus CFR06. World Journal of Microbiology and Biotechnology, 24, 805-812.
http://dx.doi.org/10.1007/s11274-007-9543-z
[25] Haas, R., Jin, B. and Zepf, F.T. (2008) Production of Poly (3-hydroxybutyrate) from Waste Potato Starch. Bioscience, Biotechnology and Biochemistry, 72, 253-256. http://dx.doi.org/10.1271/bbb.70503
[26] Quillaguamán, J., Doan-Van, T., Guzmán, H., Guzmán, D., Martín, J., Everest, A. and Hatti-Kaul, R. (2008) Poly (3-hydroxybutyrate) Production by Halomonas boliviensis in Fed-Batch Culture. Applied Microbiology and Biotechnology, 78, 227-232. http://dx.doi.org/10.1007/s00253-007-1297-x
[27] Kim, B.S. and Chang, H.N. (1998) Production of Poly (3-hydroxybutyrate) from Starch by Azotobacter chroococcum. Biotechnology Letters, 20, 109-112. http://dx.doi.org/10.1023/A:1005307903684
[28] Ribera, R.G., Monteoliva-Sanchez, M. and Ramos-Cormenzana, A. (2001) Production of Polyhidroxyalkanoates by Pseudomonas putida KT2442 Harboring pSK2665 in Wastewater from Olive Oil Mills (Alpechín). Electronic Journal of Biotechnology, 4, 11-12.
[29] Pozo, C., Mart¹nez-Toledo, M., Rodelas, B. and Gonzalez-Lopez, J. (2002) Effects of Culture Conditions on the Production of Polyhydroxyalkanoates by Azotobacter chroococcum H23 in Media Containing a High Concentration of Alpechín (Wastewater from Olive Oil Mills) as Primary Carbon Source. Journal of Biotechnology, 97, 125-131.
http://dx.doi.org/10.1016/S0168-1656(02)00056-1
[30] Bhubalan, K., Lee, W.H., Loo, C.Y., Yamamoto, T., Tsuge, T., Doi, Y. and Sudesh, K. (2008) Controlled Biosynthesis and Characterization of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) from Mixtures of Palm Kernel Oil and 3HV-Precursors. Polymer Degradation and Stability, 93, 17-23.
http://dx.doi.org/10.1016/j.polymdegradstab.2007.11.004
[31] Jiang, Y., Song, X., Gong, L., Li, P., Dai, C. and Shao, W. (2008) High Poly(β-hydroxybutyrate) Production by Pseudomonas fluorescens A2a5 from Inexpensive Substrates. Enzyme and Microbial Technology, 42, 167-172.
http://dx.doi.org/10.1016/j.enzmictec.2007.09.003
[32] Yan, S., Tyagi, R. and Surampalli, R. (2006) Polyhydroxyalkanoates (PHA) Production Using Wastewater as Carbon Source and Activated Sludge as Microorganisms. Water Science and Technology, 53, 175-180.
http://dx.doi.org/10.2166/wst.2006.193
[33] Koller, M., Bona, R., Braunegg, G., Hermann, C., Horvat, P., Kroutil, M., Martinz, J., Neto, J., Pereira, L. and Varila, P. (2005) Production of Polyhydroxyalkanoates from Agricultural Waste and Surplus Materials. Biomacromolecules, 6, 561-565. http://dx.doi.org/10.1021/bm049478b
[34] Verlinden, R.A.J., Hill, D.J., Kenward, M.A., Williams, C.D., Piotrowska-Seget, Z. and Radecka, I.K. (2011) Production of Polyhydroxyalkanoates from Waste Frying Oil by Cupriavidus necator. AMB Express, 1, 1-8.
http://dx.doi.org/10.1186/2191-0855-1-11
[35] Koutinas, A., Wang, R. and Webb, C. (2004) Restructuring Upstream Bioprocessing: Technological and Economic Aspects for Production of a Generic Microbial Feedstock from Wheat. Biotechnology and Bioengineering, 85, 524-538.
http://dx.doi.org/10.1002/bit.10888
[36] Yu, J. and Stahl, H. (2008) Microbial Utilization and Biopolyester Synthesis of Bagasse Hydrolysates. Bioresource Technology, 99, 8042-8048. http://dx.doi.org/10.1016/j.biortech.2008.03.071
[37] Law, J.H. and Slepecky, R.A. (1961) Assay of Poly-β-hydroxybutyric Acid. Journal of
Bacteriology, 82, 33-36.
[38] Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P. and Smith, F. (1956) Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry, 28, 350-356.
http://dx.doi.org/10.1021/ac60111a017
[39] Kjeldahl, J. (1883) A New Method for the Determination of Nitrogen in Organic Matter. Zeitschrift für Analytische Chemie, 22, 366-382. http://dx.doi.org/10.1007/BF01338151
[40] Kessler, B. and Witholt, B. (2001) Factors Involved in the Regulatory Network of Polyhydroxyalkanoate Metabolism. Journal of Biotechnology, 86, 97-104. http://dx.doi.org/10.1016/S0168-1656(00)00404-1
[41] Ersus, S. and Cam, M. (2007) Determination of Organic Acids, Total Phenolic Content and Antioxidant Capacity of Sour Citrus aurantium Fruit. Chemistry of Natural Compounds, 43, 607-609.
http://dx.doi.org/10.1007/s10600-007-0203-1
[42] Ververis, C., Georghiou, K., Danielidis, D., Hatzinikolaou, D.G., Santas, R. and Corleti, V. (2007) Cellulose, Hemicelluloses, Lignin and Ash Content of Some Organic Materials and Their Suitability for Use as Paper Pulp Supplements. Bioresource Technology, 98, 296-301.
http://dx.doi.org/10.1016/j.biortech.2006.01.007
[43] Taherzadeh, M.J. and Karimi, K. (2008) Pretreatment of Lignocellulosic Wastes to Improve Ethanol and Biogas Production: A Review. International Journal of Molecular Sciences, 9, 1621-1651.
http://dx.doi.org/10.3390/ijms9091621
[44] De Aragao, G.M.F., Netto, W.S., Ienczak, J.L., Fiorese, M.L., Dalcaton, F., Schmidt, F., Deucher, R., Vecchi, C. and Rodrigues, R.C. (2008) Preparation of PHA (Polyhydroxyalkanoates) from a Citric Residue. Google Patents.
[45] Page, W.J. (1992) Production of Poly-β-hydroxybutyrate by Azotobacter vinelandii UWD in Media Containing Sugars and Complex Nitrogen Sources. Applied Microbiology and Biotechnology, 38, 117-121.
http://dx.doi.org/10.1007/BF00169430
[46] Lee, S.Y. and Chang, H.N. (1994) Effect of Complex Nitrogen Source on the Synthesis and Accumulation of Poly (3-hydroxybutyric acid) by Recombinant Escherichia Coli in Flask and Fed-Batch Cultures. Journal of Environmental Polymer Degradation, 2, 169-176. http://dx.doi.org/10.1007/BF02067442
[47] Aldor, I.S. and Keasling, J.D. (2003) Process Design for Microbial Plastic Factories: Metabolic Engineering of Polyhydroxyalkanoates. Current Opinion in Biotechnology, 14, 475-483.
http://dx.doi.org/10.1016/j.copbio.2003.09.002
[48] Reddy V.S., Thirumala, M. and Mahmood, S.K. (2009) Production of P(3HB) and P(3HB-co3HV) Biopolymers by Bacillus megaterium Strain OU303A Isolated from Municipal Sewage Sludge. World Journal of Microbiology and Biotechnology, 25, 391-397.
http://dx.doi.org/10.1007/s11274-008-9903-3
[49] Tajima, K., Igari, T., Nishimura, D., Nakamura, M., Satoh, Y. and Munekata, M. (2003) Isolation and Characterization of Bacillus sp. INT005 Accumulating Polyhydroxyalkanoate (PHA) from Gas Field Soil. Journal of Bioscience and Bioengineering, 95, 77-81.
http://dx.doi.org/10.1016/S1389-1723(03)80152-4
[50] Thirumala, M., Reddy, S.V. and Mahmood, S. (2010) Production and Characterization of P(3HB) from Two Novel Strains of Bacillus sp. Isolated from Soil and Activated Sludge. Journal of Industrial Microbiology & Biotechnology, 37, 271-278.
http://dx.doi.org/10.1007/s10295-009-0670-4
[51] Yilmaz, M. and Beyatli, Y. (2005) Poly-b-hydroxybutyrate (P(3HB)) Production by a Bacillus cereus M5 Strain in Sugar Beet Molasses. Zuckerindustrie, 130, 109-112.
[52] Khiyami, M.A., Al-Fadual, S.M. and Bahklia, A.H. (2011) Polyhydroxyalkanoates Production via Bacillus Plastic Composite Support (PCS) Biofilm and Date Palm Syrup. Journal of Medicinal Plant Research, 5, 3312-3320
[53] Ferreira-Leitao, V., Gottschalk, L.M.F., Ferrara, M.A., Nepomuceno, A.L., Molinari, H.B.C. and Bon, E.P.S. (2010) Biomass Residues in Brazil: Availability and Potential Uses. Waste and Biomass Valorization, 1, 65-76. http://dx.doi.org/10.1007/s12649-010-9008-8
[54] Erkut, Y. (2011) Turkey Citrus Annual Report, 2011. Global Agricultural Information Network, USDA Foreign Agricultural Service.

  
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