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A new risk assessment method for evaluation of oxidative chemicals using catalase mutant mouse primary hepatocytes

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DOI: 10.4236/health.2011.35050    3,654 Downloads   7,155 Views  

ABSTRACT

We examined the possibility of developing a new risk assessment method for potentially hazardous chemicals by using mouse primary hepatocytes from acatalasemic mice (Csb) and the wild-type (Csa) as predictive model. Chemical-induced cytotoxicities, such as hydrogen peroxide and lawsone, a main hair dye ingredient of henna, were examined. We observed the differences in cell survival between the Csa and Csb in a dose-dependant manner after treatment with either hydrogen peroxide or lawsone, supporting the usefulness of this newly established method for hazard identification of oxidative chemicals in a risk assessment process. More chemicals will be tested to confirm the usefulness of this method for the preliminary screening of oxidative chemicals before animal experimentation.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Wang, D. , Ishikawa, Y. , Miyazaki, M. , Fujita, H. , Tsutsui, K. , Sano, K. , Masuoka, N. and Ogino, K. (2011) A new risk assessment method for evaluation of oxidative chemicals using catalase mutant mouse primary hepatocytes. Health, 3, 288-291. doi: 10.4236/health.2011.35050.

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