Share This Article:

Action of Gaseous Nitric Oxide on Some Physical and Chemical Parameters of Human Blood Samples

Abstract Full-Text HTML Download Download as PDF (Size:918KB) PP. 675-681
DOI: 10.4236/jbise.2014.79067    2,461 Downloads   3,107 Views   Citations

ABSTRACT

We studied the metabolic changes induced by gaseous nitric oxide in whole blood samples in vitro. Blood samples were collected from healthy donors (Nizhny Novgorod station of blood transfusion). We carried out the direct bubbling of blood samples (n = 14) with gaseous flow with NO in a special appliance. We modeled standard conditions using the apparatus “Plazon” (concentration NO 800 mcg/l). Middle power of gas flow was used. The blood sparging time was 2 min, and exposition time lasted 3 min. Every blood sample volume was 5 ml. All the parameters were controlled before and after blood processing with NO. We tested lactate dehydrogenase activity in direct and reverse reactions spectrometrically by G. A. Kochetov’s method. Aldehyde dehydrogenase activity was examined by B. M. Kershnhots’s and E. V. Serkina’s methods, superoxide dismutase—by T. V. Sirota’s technology. Total protein level was examined by modified Louri’s method. The concentration of lactate was tested with the automatic analyzer “SuperGL Ambulance”. The indices of acidbase balance and blood gases partial pressure were estimated with special analyzer “ABL-77”. Additional control of energy metabolism changes was accomplished with derivative parameters, such as coefficient of energy reaction balance and coefficient of substrate provision. Different changes of blood physical and chemical parameters are induced by NO-processing which was fixed in our experiments. There is an inhibition of erythrocytes energy metabolism, decreasing of plasma antioxidant reserves, moderate ionic disorders and of acid-base misbalance in blood samples in vitro. Besides, according to the indirect signs, the used regimen of NO-processing mainly affected erythrocytes, and stipulated methemoglobin formation. These data testify that the used dose of gaseous nitric oxide is too high for investigated human blood. In our opinion, registered negative effects of free NO may be eliminated by bound nitric oxide use (first of all in its natural form—dinitrosyl-iron complexes).

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Martusevich, A. , Soloveva, A. , Peretyagin, S. and Vanin, A. (2014) Action of Gaseous Nitric Oxide on Some Physical and Chemical Parameters of Human Blood Samples. Journal of Biomedical Science and Engineering, 7, 675-681. doi: 10.4236/jbise.2014.79067.

References

[1] Gryglewsky, R.J. and Minuz P. (2001) Nitric Oxide. Basic Research and Clinical Application. IOS Press, Amsterdam, Berlin, Oxford, Tokyo, Washington.
[2] Murad, F. (1994) The Role of Nitric Oxide in Modulating Guanylyl Cyclase. Neurotransmissions, 10, 1-4.
[3] Vanin, A.F. (2009) Dinitrosyl-Iron Complexes with Thiolate Ligands: Physico-Chemistry, Biochemistry and Physiology. Nitric Oxide: Biology and Chemistry, 21, 136-149.
http://dx.doi.org/10.1016/j.niox.2009.03.005
[4] Chazov, E.I., Rodnenkov, O.V., Zorin, A.V., et al. (2012) Hypotensive Effect of Oxacom Containing a Dinitrosyl Iron Complex with Glutathione. Animal Studies and Clinical Trials on Healthy Volunteers. Nitric Oxide: Biology and Chemistry, 26, 148-156.
http://dx.doi.org/10.1016/j.niox.2012.01.008
[5] Van Faassen, E. and Vanin, A.F. (2007) Radicals for Life: The Various Forms of Nitric Oxide. Elsevier, Amsterdam.
[6] Shekhter, A.B., Serezhenkov, V.A., Rudenko, T.G., et al. (2005) Beneficial Effect of Gaseous Nitric Oxide on the Healing of Skin Wounds. Nitric Oxide: Biology and Chemistry, 12, 210-219.
http://dx.doi.org/10.1016/j.niox.2005.03.004
[7] Martusevich, A.K., Peretyagin, S.P. and Ivannikova, E.V. (2012) Physical and Chemical Properties of Physiological Solution at Action of Oxygen and Nitrogen Reactive Species. Fundamental Research, 11, 197-201.
[8] Duarte, S., Kuo, S.P., Murata, R.M., et al. (2011) Air Plasma Effect on Dental Disinfection. Physics of Plasmas, 18, 073501-073507.
http://dx.doi.org/10.1063/1.3606486
[9] Lai, W., Lai, H., Kuo, S.P., et al. (2005) Decontamination of Biological Warfare Agents by a Microwave Plasma Torch. Physics of Plasmas, 12, 023501-023506.
http://dx.doi.org/10.1063/1.1843131
[10] Kuo, S.P. (2012) Air Plasma for Medical Applications. J. Biomedical Science and Engineering, 5, 481-495.
http://dx.doi.org/10.4236/jbise.2012.59061
[11] Baxter, H.C., Campbell, G.A., Whittaker, A.G., et al. (2005) Elimination of TSE Infectivity and Decontamination of Surgical Instruments Using RF Gas-Plasma Treatment. Journal of General Virology, 86, 2393-2399.
http://dx.doi.org/10.1099/vir.0.81016-0
[12] Kuo, S.P., Tarasenko, O., Popovic, S. and Levon, K. (2006) Killing of Bacterial Spores Contained in a Paper Envelope by a Microwave Plasma Torch. IEEE Transactions on Plasma Science, 34, 1275-1280.
http://dx.doi.org/10.1109/TPS.2006.878389
[13] Kuo, S.P., Chen, C.Y., Lin, C.S. and Chiang, S.H. (2010) Wound Bleeding Control by Low Temperature Air Plasma. IEEE Transactions on Plasma Science, 38, 1908-1914.
http://dx.doi.org/10.1109/TPS.2010.2047028
[14] Kuo, S.P., Chen, C.Y., Lin, C.S. and Chiang, S.H. (2012) Applications of Air Plasma for Wound Bleeding Control and Healing. IEEE Transactions on Plasma Science, 40, 1117-1123.
http://dx.doi.org/10.1109/TPS.2012.2184142
[15] Brune, B. and Hanstein, K. (1998) Rapid Reversibility of Nitric Oxide Induced Platelet Inhibition. Thrombosis Research, 90, 83-91.
http://dx.doi.org/10.1016/S0049-3848(98)00024-3
[16] Gries, A., Bode, C., Peter, K., et al. (1998) Inhaled Nitric Oxide Inhibits Human Platelet Aggregation, p-Selectin Expression, and Fibrinogen Binding in Vitro and in Vivo. Circulation, 97, 1481-1487.
http://dx.doi.org/10.1161/01.CIR.97.15.1481
[17] Nong, Z., Hoylaerts, M., Van Pelt, N., et al. (1997) Nitric Oxide Inhalation Inhibits Platelet Aggregation and Platelet-Mediated Pulmonary Thrombosis in Rats. Circulation Research, 81, 865-869.
http://dx.doi.org/10.1161/01.RES.81.5.865
[18] Weber, A., Strobach, H. and Schror, K. (1993) Direct Inhibition of Platelet Function by Organic Nitrates via Nitric Oxide Formation. European Journal of Pharmacology, 247, 29-37.
http://dx.doi.org/10.1016/0922-4106(93)90134-U
[19] Giliano, N.Ya., Konevega, L.V., Noskin, L.A., et al. (2011) Dinitrosyl Iron Complexes with Thiol-Containing Ligands and Apoptosis: Studies with HeLa Cell Cultures. Nitric Oxide: Biology and Chemistry, 24, 151-159.
http://dx.doi.org/10.1016/j.niox.2011.02.005
[20] Mathisen, D.J., Kuo, E.Y., Hahn, C., et al. (1998) Inhaled Nitric Oxide for Adult Respiratory Distress Syndrome after Pulmonary Resection. The Annals of Thoracic Surgery, 66, 1894-1902.
http://dx.doi.org/10.1016/S0003-4975(98)01167-9
[21] Ricciardi, M.J., Knight, B.P., Martinez, F.J. and Rubenfire, M. (1998) Inhaled Nitric Oxide in Primary Hypertension: A Safe and Effective Agent for Predicting Response to Nifedipine. Journal of the American College of Cardiology, 32, 1068-1073.
http://dx.doi.org/10.1016/S0735-1097(98)00361-1
[22] van der Vliet, A., et al. (1997) Formation of Reactive Nitrogen Species during Peroxidase-Catalyzed Oxidation of Nitrite. A Potential Additional Mechanism of Nitric Oxide-Dependent Toxicity. The Journal of Biological Chemistry, 272, 7617-7625.
http://dx.doi.org/10.1074/jbc.272.12.7617
[23] Onufriev, M.V. (2010) Nitrosative Stress in the Brain: Autoantibodies to Nitrotyrosine in Liquor as Potential Marker, Neurochemistry, 27, 257-263.
[24] Martusevich, A.K. and Peretyagin, S.P. (2013) Modification of Blood Plasma Crystallogenesis with Nitrogen Oxide Processing. Biophysics, 58, 1038-1042.
http://dx.doi.org/10.1134/S0006350913060134
[25] Hall, C.N. and Garthwaite, J. (2009) What Is the Real Physiological NO Concentration in Vivo? Nitric Oxide: Biology and Chemistry, 12, 92-103.
http://dx.doi.org/10.1016/j.niox.2009.07.002
[26] Martusevich, A.K., Soloveva, A.G., Peretyagin, S.P. and Mitrofanov V.N. (2013) Estimation of Some Physical Factors Influence on Blood Energy Metabolism in Vitro. Biomedicine, 1, 103-108.
[27] Schweitzer, C. and Schmidt, R. (2003) Physical Mechanisms of Generation and Deactivation of Singlet Oxygen. Chemical Reviews, 103, 1685-1757.
[28] Genestra, M. (2007) Oxyl Radicals, Redox-Sensitive Signalling Cascades and Antioxidants. Cellular Signalling, 19, 1807-1819.
http://dx.doi.org/10.1016/j.cellsig.2007.04.009
[29] Halliwell, B. and Gutteridge, J.M.C. (1999) Free Radicals in Biology and Medicine. Oxford University Press, Oxford.
[30] He, X., Azarov, I., Jeffers A., et al. (2008) The Potential of Angelis Salt to Decrease Nitric Oxide Scavenging by Plasma Hemoglobin. Free Radical Biology Medicine, 44, 1420-1432.
http://dx.doi.org/10.1016/j.freeradbiomed.2007.12.038
[31] Shekhter, A.B., Rudenko, T.G., Serezhenkov, V.A. and Vanin, A.F. (2007) Dinitrosyl Iron Complexes with Thiol Ligands Promote Skin Wound Healing in Animals. Biophysics, 52, 539-547.
http://dx.doi.org/10.1134/S0006350907050120
[32] Shumaev, K.B., Gubkin, A.A., Serezhenkov, V.A., et al. (2008) Interaction of Reactive Oxygen and Nitrogen Species with Albumin- and Methemoglobin-Bound Dinitrosyl Iron Complexes. Nitric Oxide: Biology and Chemistry, 18, 37-46.
http://dx.doi.org/10.1016/j.niox.2007.09.085

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.