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Effects of AGE Inhibition with Aminoguanidine in a Diabetic db/db Mouse Wound Model

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DOI: 10.4236/jdm.2014.42017    2,516 Downloads   3,522 Views   Citations

ABSTRACT

Advanced glycation end products (AGEs) react non-enzymatically with tissue proteins to form irreversible structures involved in atherosclerosis, nephropathy, retinopathy, neuropathy, and wound healing. Studies on AGE-inhibitors have demonstrated possible prevention of diabetes complications. The present open label study was conducted on aminoguanidine (AGu), an inhibitor of AGE-formation, to examine potential effects on wound healing in diabetes type 2-like db/db mice during 5 - 6 weeks. The animals were divided into 4 groups: AGu from the day of wounding (day 0) topically and/or systemically in drinking water (1 g/L; group 1, n = 13); AGu 1 g/L in drinking water from 7 weeks prior to day 0 (group 2, n = 21); AGu 5 g/L in drinking water from 9 - 11 weeks prior to day 0 (group 3, n = 6); placebo controls (group 4, n = 8). Results: Glycated hemoglobin (A1C) was significantly lower in group 3 compared to the other groups (P < 0.05). Percentage change in A1C and body weight from baseline to the end of the experiment were both related to the AGu doses (1 or 5 g/L; A1C-change, P = 0.01; weight-change, P = 0.04, both for linear trend across groups 4, 2, and 3, respectively). Even so, percentage wound closure was not improved in the AGu-treated groups compared to controls (P ≥ 0.8).

Conflicts of Interest

The authors declare no conflicts of interest.

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Berdal, M. and Jenssen, T. (2014) Effects of AGE Inhibition with Aminoguanidine in a Diabetic db/db Mouse Wound Model. Journal of Diabetes Mellitus, 4, 107-114. doi: 10.4236/jdm.2014.42017.

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