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Development of a Genetic Transformation Method for Seabuckthorn (Hippophae rhamnoides L.)

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DOI: 10.4236/ajps.2014.55067    3,456 Downloads   4,577 Views  

ABSTRACT

Seabuckthorn (Hippophae rhamnoides L.) is a dioecious plant with berries containing high amounts of several bioactive compounds with nutritional and medicinal traits. It is also planted to control soil erosion. A genetic transformation procedure will facilitate studies of the control of plant development and interactions with symbionts and pathogens, and will provide a tool for plant breeding. Here, we present a particle bombardment method for transforming seabuckthorn. The early stages of induced adventitious shoots from roots were chosen as a novel target tissue for the transformation procedure. The root system was bombarded with gold particles coated with plasmid pRT99gus containing genes for plant kanamycin resistance and for β-glucuronidase expression, and shoots were regenerated under kanamycin selection. PCR analysis of the regenerated transformed lines confirmed the presence of a 603 bp gus (uidA) gene fragment and a 1.5 kb fragment from the 35S promoter in three shoots from independent transformation events.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Sriskandarajah, S. , Clapham, D. and Lundquist, P. (2014) Development of a Genetic Transformation Method for Seabuckthorn (Hippophae rhamnoides L.). American Journal of Plant Sciences, 5, 528-534. doi: 10.4236/ajps.2014.55067.

References

[1] Singh, V (2005) Sea buckthorn (Hippophae L.). A Multipurpose Wonder Plant. Vol. 2, Singh V. Daya Publishing House; New Delhi.
[2] Kalia, R.K., Singh, R., Rai, M.K., Mishra, G.P., Singh, S.R. and Dhawan, A.K. (2011) Biotechnological Interventions in Seabuckthorn (Hippophae L.): Current Status and Future Prospects. Trees, 25, 559-575.
http://dx.doi.org/10.1007/s00468-011-0543-0
[3] Sun, Y.-L. and Hong, S.-K. (2012) Effect of Chitinase on Resistance to Fungal Pathogens in Sea Buckthorn, Hippophae rhamnoides, and Cloning of Class I and III Chitinase Genes. Biochemical Genetics, 50, 600-615.
http://dx.doi.org/10.1007/s10528-012-9504-6
[4] Vershinina, Z.R., Baimiev, A.K. and Chemeris, A.V. (2010) Symbiotic Reactions of Sea-Buckthorn Roots Transformed with the Pea Lectin Gene. Russian Journal of Plant Physiology, 57, 101-109.
http://dx.doi.org/10.1134/S1021443710010140
[5] Le, Q., Bogusz, D., Gherbi, H., Lappartient, A., Duhoux, E. and Franche, C. (1996) Agrobacterium tumefaciens Gene Transfer to Casuarina glauca, a tropical nitrogen-fixing tree. Plant Science, 118, 57-69.
http://dx.doi.org/10.1016/0168-9452(96)04386-5
[6] Franche, C., Diouf, D., Le, Q., Bogusz, D., N’Diaye, A., Gherbi, H., Gobé, C. and Duhoux, E. (1997) Genetic Transformation of the Actinorhizal Tree Allocasuarina verticillata by Agrobacterium tumefaciens. The Plant Journal, 11, 897-904. http://dx.doi.org/10.1046/j.1365-313X.1997.11040897.x
[7] Sriskandarajah, S. and Lundquist, P.-O. (2009) High Frequency Shoot Organogenesis and Somatic Embryogenesis in Juvenile and Adult Tissues of Seabuckthorn (Hippophae rhamnoides L.). Plant Cell, Tissue and Organ Culture (PCTOC), 99, 259-268. http://dx.doi.org/10.1007/s11240-009-9597-8
[8] Clapham, D., Elfstrand, M., Sabala, I., Von Arnold, S., Demel, P. and Koop, H.-U. (2000) Gene Transfer by Particle Bombardment to Embryogenic Cultures of Picea abies and the Production of Transgenic Plantlets. Scandinavian Journal of Forest Research, 15, 151-160. http://dx.doi.org/10.1080/028275800750014957
[9] Wadenbäck, J., von Arnold, S., Egertsdotter, U., Walter, M.H., Grima-Pettenati, J., Goffner, D., Gellerstedt, G., Gullion, T. and Clapham, D. (2008) Lignin Biosynthesis in Transgenic Norway Spruce Plants Harboring an Antisense Construct for Cinnamoyl CoA Reductase (CCR). Transgenic Research, 17, 379-392.
http://dx.doi.org/10.1007/s11248-007-9113-z
[10] Parasharami, V.A., Naik, V.B., von Arnold, S., Nadgauda, R.S. and Clapham, D.H. (2006) Stable Transformation of Mature Zygotic Embryos and Regeneration of Transgenic Plants of Chir Pine (Pinus roxburghii Sarg). Plant Cell Reports, 24, 708-714. http://dx.doi.org/10.1007/s00299-005-0019-z
[11] Shirgurkar, M.V., Naik, V.B., Von Arnold, S., Nadgauda, R.S. and Clapham, D. (2006) An Efficient Protocol for Genetic Transformation and Shoot Regeneration of Turmeric (Curcuma longa L.) via Particle Bombardment. Plant Cell Reports, 25, 112-116. http://dx.doi.org/10.1007/s00299-005-0033-1
[12] Bruvelius, A. (2003) Sea Buckthorn Cultivation in Baltic States. Proceeding of the 1st Congress of the International Seabuckthorn Association, Berlin, 14-18 September 2003, 64-66.
[13] Lloyd, G. and McCown, B. (1981) Commercially Feasible Micropropagation of Mountain Laurel, Kalmia latifolia, by Use of Shoot Tip Culture. Combined Proceedings—International Plant Propagator’s Society, 30, 421-427.
[14] Töpfer, R., Schell, J. and Steinbiss, H. (1988) Versatile Cloning Vectors for Transient Gene Expression and Direct Gene Transfer in Plant Cells. Nucleic Acids Research, 16, 8725. http://dx.doi.org/10.1093/nar/16.17.8725
[15] Tartoff, K.D. and Hobbs, C.A. (1987) Improved Media for Growing Plasmid and Cosmid Clones. Bethesda Research Laboratories Focus, 9, 12.
[16] Sambrook, J., Fritsch, E. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
[17] Sivamani, E., DeLong, R.K. and Qu, R. (2009) Protamine-Mediated DNA Coating Remarkably Improves Bombardment Transformation Efficiency in Plant Cells. Plant Cell Reports, 28, 213-221.
http://dx.doi.org/10.1007/s00299-008-0636-4
[18] Doyle, J.J. and Doyle, J.L. (1990) A Rapid Total DNA Preparation Procedure for Fresh Plant Tissue. Focus, 12, 13-15.
[19] Azevedo, H., Lino-Neto, T. and Tavares, R. (2003) An Improved Method for High-Quality RNA Isolation from Needle of Adult Maritime Pine Trees. Plant Molecular Biology Reporter, 21, 333-338. http://dx.doi.org/10.1007/BF02772582
[20] Lummerding, L. (2001) Agri-Food Innovation Fund Project. Prairie Plant Systems, Saskatoon.
[21] Serres, R., McCown, B. and Zeldin, E. (1997) Detectable β-Glucuronidase Activity in Transgenic Cranberry Is Affected by Endogenous Inhibitors and Plant Development. Plant Cell Reports, 16, 641-646.

  
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