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Microbial and enzyme technology: An efficient and convenient method for MiniPrep analysis of recombinant plasmids

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DOI: 10.4236/jbise.2014.73013    3,387 Downloads   4,690 Views  

ABSTRACT

Minipreparation (MiniPrep) analysis is an essential step for obtaining a recombinant plasmid that carries a DNA insert containing a gene of interest. The most commonly used method for this involves cultivation of transformed Escherichia coli (E. coli) in liquid medium, brief centrifugation for precipitation of bacterial pellets, and subsequent lysis of the pellets. This process is time-consuming and laborious, especially when the sample number is high. Here, we describe a more convenient method for MiniPrep analysis that utilizes solid medium-based cultivation of bacteria.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Sato, M. , Inada, E. , Saitoh, I. and Matsumoto, Y. (2014) Microbial and enzyme technology: An efficient and convenient method for MiniPrep analysis of recombinant plasmids. Journal of Biomedical Science and Engineering, 7, 105-107. doi: 10.4236/jbise.2014.73013.

References

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[3] QIAGEN Plasmid Purification System in Qiagen Home Page. http://www.ebiotrade.com/buyf/productsf/qiagen/QIAGEN_plasmid_purification_system.htm
[4] Sato, M., Akasaka, E., Saitoh, I., Ohtsuka, M., Nakamura, S., Sakurai, T. and Watanabe, S. (2012) A simplified protocol for the semi-large scale recovery of plasmids from Escherichia coli grown on agar plates. Journal of Biomedical Science and Engineering, 5, 406-408.
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[6] Mini-prep Protocol.
http://irc.igd.cornell.edu/Protocols/Mini-prep.htm

  
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