Share This Article:

Intramedullary Spinal Cord Glioma Following Microinjection of Glioblastoma Cell Line C6 in Rats

Abstract Full-Text HTML XML Download Download as PDF (Size:1113KB) PP. 43-46
DOI: 10.4236/ojmn.2014.41010    2,805 Downloads   4,384 Views   Citations

ABSTRACT

Background: This paper describes the establishment of a rat intramedullary spinal cord tumor (IMSCT) model and histopathological characterization of the tumor model. Methods: Fourteen male Wistar rats were randomized into two groups. The rats in group 1 (control group, n = 7) received a 5 μl intramedullary injection of serum physiologic (SF). Those in group 2 (experimental group, n= 7) received a 5 μl intramedullary implantation of media containing 5 × 105 C6 glioma cells. The animals were sacrificed for histopathological examination at 21 days. Results: The control group showed normal functional and histopathological findings. The group 2 rats implanted with C6 glioblastoma cells developed hind-limb paraplegia. Pathological sections confirmed intramedullary C6 glioblastoma invading the spinal cord. Conclusions: A rat C6 IMSCT model was successfully established. This model may be useful in increasing understanding of intramedullary spinal cord gliomas in humans.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Y. Dagistan, G. Ozyalvacli, T. Firat, K. Yagmurlu and E. Terzi, "Intramedullary Spinal Cord Glioma Following Microinjection of Glioblastoma Cell Line C6 in Rats," Open Journal of Modern Neurosurgery, Vol. 4 No. 1, 2014, pp. 43-46. doi: 10.4236/ojmn.2014.41010.

References

[1] D. C. Miller, “Surgical Pathology of Intramedullary Spinal Cord Neoplasms,” Journal of Neurooncology, Vol. 47, No. 3, 2000, pp. 189-194. http://dx.doi.org/10.1023/A:1006496204396
[2] A. Raco, V. Esposito, J. Lenzi, M. Piccirilli, R. Delfini and G. Cantore, “Long-Term Follow-Up of Intramedullary Spinal Cord Tumors: A Series of 202 Cases,” Neurosurgery, Vol. 56, No. 5, 2005, pp. 972-981.
[3] T. H. Schwartz and P. C. McCormick, “Intramedullary Ependymomas: Clinical Presentation, Surgical Treatment Strategies and Prognosis,” Journal of Neurooncology, Vol. 47, No. 3, 2000, pp. 211-218. http://dx.doi.org/10.1023/A:1006414405305
[4] A. A. Andrews, L. Enriques, J. Renaudin and U. Tomiyasu, “Spinal Intramedullary Glioblastoma with Intracranial Seeding,” Archives of Neurology, Vol. 35, No. 4, 1978, pp. 244-245. http://dx.doi.org/10.1001/archneur.1978.00500280062013
[5] A. Raco, M. Piccirilli, A. Landi, J. Lenzi, R. Delfini and G. Cantore, “High-Grade Intramedullary Astrocytomas: 30 Years’ Experience at the Neurosurgery Department of the University of Rome ‘Sapienza’,” Journal of Neurosurgical Spine, Vol. 12, No. 2, 2010, pp. 144-153. http://dx.doi.org/10.3171/2009.6.SPINE08910
[6] S. Yang, X. Yang and G. Hong, “Surgical Treatment of One Hundred Seventy-Four Intramedullary Spinal Cord Tumors,” Spine (Phila Pa 1976), Vol. 34, No. 24, 2009, pp. 2705-2710. http://dx.doi.org/10.1097/BRS.0b013e3181b43484
[7] R. F. Barth, “Rat Brain Tumor Models in Experimental Neuro-Oncology: The 9L, C6, T9, F98, RG2 (D74), RT-2 and CNS-1 Gliomas,” Journal of Neurooncology, Vol. 36, No. 1, 1998, pp. 91-102. http://dx.doi.org/10.1023/A:1005805203044
[8] A. T. Parsa, J. H. Chi, F. L. Acosta Jr., et al., “Intramedullary Spinal Cord Tumors: Molecular Insights and Surgical Innovation,” Clinical Neurosurgery, Vol. 52, No. 49-51, 2005, pp. 76-84.
[9] K. A. Schellinger, J. M. Propp, J. L. Villano and B. J. McCarthy, “Descriptive Epidemiology of Primary Spinal Cord Tumors,” Journal of Neurooncology, Vol. 87, No. 2, 2008, pp. 173-179. http://dx.doi.org/10.1007/s11060-007-9507-z
[10] G. Kopelson, R. M. Linggood, G. M. Kleinman, J. Doucette and C. C. Wang, “Management of Intramedullary Spinal Cord Tumors,” Radiology, Vol. 135, No. 2, 1980, pp. 473-479.
[11] G. Kopelson and R. M. Linggood, “Intramedullary Spinal Cord Astrocytoma versus Glioblastoma: The Prognostic Importance of Histologic Grade,” Cancer, Vol. 50, No. 4, 1982, pp. 732-735. http://dx.doi.org/10.1002/1097-0142(19820815)50:4<732::AID-CNCR2820500418>3.0.CO;2-0
[12] A. Di Marco, C. Griso, R. Pradella, F. Campostrini and G. F. Garusi, “Postoperative Management of Primary Spinal Cord Ependymomas,” Acta Oncology, Vol. 27, No. 4, 1988, pp. 371-375. http://dx.doi.org/10.3109/02841868809093557
[13] M. C. Chamberlain and T. L. Tredway, “Adult Primary Intradural Spinal Cord Tumors: A Review,” Current Neurology and Neuroscience Reports, Vol. 11, No. 3, 2011, pp. 320-328. http://dx.doi.org/10.1007/s11910-011-0190-2
[14] J. W. Henson, “Spinal Cord Gliomas,” Current Opinion in Neurology, Vol. 14, No. 6, 2001, pp. 679-682. http://dx.doi.org/10.1097/00019052-200112000-00001
[15] J. L. Slooff, J. W. Kernohan and C. S. MacCarty, “Primary Intramedullary Tumors of the Spinal Cord and Filum Terminale,” WB Saunders Company, Philadelphia, 1964.
[16] G. B. Rodrigues, J. N. Waldron, S. Wong and N. J. Laperriere, “A Retrospective Analysis of 52 Cases of Spinal Cord Glioma Managed with Radiation Therapy,” International Journal of Radiation Oncology, Biology and Physics, Vol. 48, No. 3, 2001, pp. 837-842. http://dx.doi.org/10.1016/S0360-3016(00)00690-8
[17] B. S. Carson, J. H. Anderson, S. A. Grossman, J. Hilton, C. L. White 3rd, O. M. Colvin, et al., “Improved Rabbit Brain Tumor Model Amenable to Diagnostic Radiographic Procedures,” Neurosurgery, Vol. 11, No. 5, 1982, pp. 603-608. http://dx.doi.org/10.1227/00006123-198211000-00003
[18] W. Griffitt, R. P. Glick and T. Lichtor, “Development of a New Mouse Brain Tumor Model Using Implantable Micro Cannulas,” Journal of Neurooncology, Vol. 41, No. 2, 1999, pp. 117-120. http://dx.doi.org/10.1023/A:1006115221761
[19] G. I. Jallo, M. Penno and L. Sukay, “Experimental Models of Brainstem Tumors: Development of a Neonatal Rat Model,” Child’s Nervous System, Vol. 21, No. 5, 2005, pp. 399-403. http://dx.doi.org/10.1007/s00381-004-1100-6
[20] M. Salcman, E. Botero, K. C. Rao, R. D. Broadwell and E. Scott, “Intramedullary Canine Spinal Cord Tumor Model,” Journal of Neurooncology, Vol. 61, No. 4, 1984, pp. 761-766. http://dx.doi.org/10.3171/jns.1984.61.4.0761
[21] G. Mavinkurve, G. Pradilla, F. G. Legnani, B. M. Tyler, C. A. Bagley and H. Brem, “A Novel Intramedullary Spinal Cord Tumor Model: Functional, Radiological, and Histopathological Characterization,” Journal of Neurosurgical Spine, Vol. 3, Journal of Neurooncology, Vol. 3, No. 2, 2005, pp. 142-148. http://dx.doi.org/10.3171/spi.2005.3.2.0142
[22] J. Caplan, G. Pradilla, A. Hdeib, B. M. Tyler, F. G. Legnani, C. A. Bagley, et al., “A Novel Model of Intramedullary Spinal Cord Tumors in Rats: Functional Progression and Histopathological Characterization,” Neurosurgery, Vol. 59, Journal of Neurooncology, Vol. 59, No. 1, 2006, pp. 193-200. http://dx.doi.org/10.1227/01.NEU.0000219276.44563.DA
[23] T. J. Ren, Z. C. Wang, Y. Z. Zhang, D. Li, H. Y. Wang and Z. Z. Li, “Establishment of Intramedullary Spinal Cord Glioma Model in Rats,” Chinical Medicine Journal (English), Vol. 123, No. 18, 2010, pp. 2580-2585.

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.