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Crocetin Prevents Amyloid β1-42-Induced Cell Death in Murine Hippocampal Cells

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DOI: 10.4236/pp.2014.51007    3,219 Downloads   4,687 Views   Citations

ABSTRACT

Crocetin is an aglycon of carotenoid extracted by saffron stigmas (Crocus sativus L.) and known to have a potent anti-oxidative effect. Amyliod β (Aβ), hallmark of Alzheimer’s disease, is reported to have neurotoxicity partly via oxidative stress. In this study, we investigated the effect of crocetin on hippocampal HT22 cell death induced by Aβ1-42. Furthermore, to clarify the mechanism underlying the protective effects of crocetin against Aβ1-42- induced cell death, we measured reactive oxygen species (ROS) production by CM-H2DCFDA kit assay. Crocetin at 1 -10 μM protected HT22 cells against Aβ1-42-induced neuronal cell death and decreased ROS production increased by Aβ1-42. These results that crocetin has the potent neuroprotective effect against Aβ1-42-induced cytotoxicity in hippocampal cells by attenuating oxidative stress, suggest that crocetin may provide a useful therapeutic strategy against Aβ-related disorders.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Y. Yoshino, M. Ishisaka, N. Umigai, M. Shimazawa, K. Tsuruma and H. Hara, "Crocetin Prevents Amyloid β1-42-Induced Cell Death in Murine Hippocampal Cells," Pharmacology & Pharmacy, Vol. 5 No. 1, 2014, pp. 37-42. doi: 10.4236/pp.2014.51007.

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