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Expression Pattern of Sucrose Transporters in Arabidopsis thaliana During Aphid (Myzus persicae) Infestation

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DOI: 10.4236/ajps.2013.412A3006    4,812 Downloads   6,276 Views   Citations

ABSTRACT

Herbivorous insects change the metabolism of the plant during their attack. Our study reports the changes in the expression pattern of sucrose transporters in response to the infestation of aphids at different time intervals. Results showed a significant enhancement in the expression pattern for six out of nine sucrose transporters in response to aphid infestation, followed by suppression after some point. During an earlier time point of infestation, the expressions of sucrose transporters were enhanced probably to compensate for the energy requirements of the damaged cell. However, suppression of sucrose transporters at a later stage may be a defense strategy of the plant to repel the aphids because at a later stage of infestation, aphids become a secondary sink. To complement our assumption, we performed aphid infestation choice and reproductive performance tests in the null mutant of one of the transporters, SUC2, which was compromised in phloem loading of sucrose. Results showed that the mutant was less preferable to aphid for choice as well as reproduction performance.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

N. Dubey, A. Idris, A. Verma, K. Chandrashekar and K. Pandey, "Expression Pattern of Sucrose Transporters in Arabidopsis thaliana During Aphid (Myzus persicae) Infestation," American Journal of Plant Sciences, Vol. 4 No. 12C, 2013, pp. 47-51. doi: 10.4236/ajps.2013.412A3006.

References

[1] C. Couldridge, H. J. Newbury, B. Ford-Lloyd, J. Bale and J. Pritchard, “Exploring Plant Responses to Aphid Feeding Using a Full Arabidopsis Microarray Reveals a Small Number of Genes with Significantly Altered Expression,” Bulletin of Entomological Research, Vol. 97, No. 5, 2007, pp. 523-532.
http://dx.doi.org/10.1017/S0007485307005160
[2] P. J. Moran and G. A. Thompson, “Molecular Responses to Aphid Feeding in Arabidopsis in Relation to Plant Defense Pathways,” Plant Physiology, Vol. 125, No. 2, 2001, pp. 1074-1085. http://dx.doi.org/10.1104/pp.125.2.1074
[3] L. A. Kempema, X. Cui, F. M. Holzer and L. L. Walling, “Arabidopsis Transcriptome Changes in Response to Phloem-Feeding Silver Leaf Whitefly Nymphs. Similarities and Distinctions in Responses to Aphids,” Plant Physiology, Vol. 143, No. 2, 2007, pp. 849-865.
http://dx.doi.org/10.1104/pp.106.090662
[4] S. Meyer, C. Lauterbach, M. Niedermeier, I. Barth, R. D. Sjolund and N. Sauer, “Wounding Enhances Expression of AtSUC3, a Sucrose Transporter from Arabidopsis Sieve Elements and Sink Tissues,” Plant Physiology, Vol. 134, No. 2, 2004, pp. 684-693.
http://dx.doi.org/10.1104/pp.103.033399
[5] A. B. Sivitz, A. Reinders, M. E. Johnson, A. D. Krentz, C. P. L. Grof, J. M. Perroux and J. M. Ward, “Arabidopsis Sucrose Transporter AtSUC9. High-Affinity Transport Activity, Intragenic Control of Expression, and Early Flowering Mutant Phenotype,” Plant Physiology, Vol. 143, No. 1, 2007, pp. 188-198.
http://dx.doi.org/10.1104/pp.106.089003
[6] J. R. Gottwald, P. J. Krysan, J. C. Young, R. F. Evert and M. R. Sussman, “Genetic Evidence for the in Planta Role of Phloem-Specific Plasma Membrane Sucrose Transporters,” Proceedings of the National Academy of Sciences USA, Vol. 97, No. 25, 2000, pp. 13979-13984.
http://dx.doi.org/10.1073/pnas.250473797
[7] A. Weise, L. Barker, C. Kühn, S. Lalonde, H. Buschmann, W. B. Frommer and J. M. Ward, “A New Subfamily of Sucrose Transporters, SUT4., with Low Affinity/High Capacity Localized in Enucleate Sieve Elements of Plants,” The Plant Cell Online, Vol. 12, No. 8, 2000, pp. 1345-1355.
[8] S. Baud, S. Wuilleme, R. Lemoine, J. Kronenberger, M. Caboche, L. Lepiniec and C. Rochat, “The AtSUC5 Sucrose Transporter Specifically Expressed in the Endosperm Is Involved in Early Seed Development in Arabidopsis,” The Plant Journal, Vol. 43, No. 6, 2005, pp. 824-836.
http://dx.doi.org/10.1111/j.1365-313X.2005.02496.x
[9] N. Sauer, A. Ludwig, A. Knoblauch, P. Rothe, M. Gahrtz and F. Klebl, “AtSUC8 and AtSUC9 Encode Functional Sucrose Transporters, but the Closely Related AtSUC6 and AtSUC7 Genes Encode Aberrant Proteins in Different Arabidopsis Ecotypes,” The Plant Journal, Vol. 40, No. 1, 2004, pp. 120-130.
http://dx.doi.org/10.1111/j.1365-313X.2004.02196.x
[10] U. Z. Hammes, D. P. Schachtman, R. H. Berg, E. Nielsen, W. Koch, L. M. McIntyre and C. G. Taylor, “Nematode-Induced Changes of Transporter Gene Expression in Arabidopsis Roots,” Molecular Plant-Microbe Interactions, Vol. 18, No. 12, 2005, pp. 1247-1257.
http://dx.doi.org/10.1094/MPMI-18-1247
[11] K. J. Livak and T. D. Schmittgen, “Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2-[Delta][Delta] CT Method,” Methods, Vol. 25, No. 4, 2001, pp. 402-408.
http://dx.doi.org/10.1006/meth.2001.1262
[12] R. Flemming and A. Retnakaran, “Evaluating Single Treatment Data with Reference to Insecticide,” Journal of Economic Entomology, Vol. 78, 1985, pp. 1179-1181.
[13] M. De Vos, V. R. Van Oosten, R. M. P. Van Poecke, J. A. Van Pelt, M. J. Pozo, M. J. Mueller, A. J. Buchala, J. P. Métraux, L. C. Van Loon and M. Dicke, “Signal Signature and Transcriptome Changes of Arabidopsis during Pathogen and Insect Attack,” Molecular Plant-Microbe Interactions, Vol. 18, No. 9, 2005, pp. 923-937.
http://dx.doi.org/10.1094/MPMI-18-0923
[14] K. Juergensen, J. Scholz-Starke, N. Sauer, P. Hess, A. J. E. Van Bel and F. M. W. Grundler, “The Companion Cell-Specific Arabidopsis Disaccharide Carrier AtSUC2 Is Expressed in Nematode-Induced Syncytia,” Plant Physiology, Vol. 131, 2003, No. 1, pp. 161-169.
[15] A. C. Srivastava, S. Ganesan, I. O. Ismail and B. G. Ayre, “Functional Characterization of the Arabidopsis AtSUC2 Sucrose/H+ Symporter by Tissue-Specific Complementation Reveals an Essential Role in Phloem Loading but Not in Long-Distance Transport,” Plant Physiology, Vol. 148, No. 1, 2008, pp. 200-211.
http://dx.doi.org/10.1104/pp.108.124776
[16] M. Birschwilks, S. Haupt, D. Hofius and S. Neumann, “Transfer of Phloem-Mobile Substances from the host Plants to the Holoparasite Cuscuta sp,” Journal of Experimental Botany, Vol. 57, No. 4, 2006, pp. 911-921.
http://dx.doi.org/10.1093/jxb/erj076
[17] J. N. Holland, W. Cheng and D. A. Crossley, “Herbivore-Induced Changes in Plant Carbon Allocation: Assessment of Below-Ground C Fluxes Using Carbon-14,” Oecologia, Vol. 107, No. 1, 1996, pp. 87-94.
http://dx.doi.org/10.1007/BF00582238
[18] F. Divol, F. Vilaine, S. Thibivilliers, J. Amselem, J. C. Palauqui, C. Kusiak and S. Dinant, “Systemic Response to Aphid Infestation by Myzus persicae in the Phloem of Apium graveolens,” Plant Molecular Biology, Vol. 57, No. 4, 2005, pp. 517-540.
http://dx.doi.org/10.1007/s11103-005-0338-z
[19] M. Morita, N. Shitan, K. Sawada, M. C. Van Montagu, D. Inzé, H. Rischer, A. Goossens, K. M. Oksman Caldentey, Y. Moriyama and K. Yazaki, “Vacuolar Transport of Nicotine Is Mediated by a Multidrug and Toxic Compound Extrusion (MATE) Transporter in Nicotiana tabacum,” Proceedings of the National Academy of Sciences USA, Vol. 106, No. 7, 2009, pp. 2447-2252.
http://dx.doi.org/10.1073/pnas.0812512106
[20] B. L. J. Ellerbrock, J. H. Kim and G. Jander, “Contribution of Glucosinolate Transport to Arabidopsis Defense Responses,” Plant Signaling & Behavior, Vol. 2, No. 4, 2007, pp. 282-283. http://dx.doi.org/10.4161/psb.2.4.4014
[21] J. M. Ward, C. Kühn, M. Tegeder and W. B. Frommer, “Sucrose Transport in Higher Plants,” International Review of Cytology, Vol. 178, 1997, pp. 41-71.
http://dx.doi.org/10.1016/S0074-7696(08)62135-X
[22] S. Chen, X. Q. Li, A. Zhao, L. Wang, X. Li, Q. Shi, M. Chen, J. Guo, J. Zhang, D. Qi and G. Liu, “Genes and Pathways Induced in Early Response to Defoliation in Rice Seedlings,” Current Issues in Molecular Biology, Vol. 11, No. 2, 2009, pp. 81-100.
[23] A. C. Srivastava, K. Dasgupta, E. Ajieren, G. Costilla, R. C. McGarry and B. G. Ayre, “Arabidopsis Plants Harbouring a Mutation in AtSUC2, Encoding the Predominant Sucrose/Proton Symporter Necessary for Efficient Phloem Transport, Are Able to Complete Their Life Cycle and Produce Viable Seed,” Annals of Botany, Vol. 104, No. 6, 2009, pp. 1121-1128.
http://dx.doi.org/10.1093/aob/mcp215

  
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