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Quantitative Detection of Inositol Hexakisphosphate (InsP6) in Crop Plants Using Polyacrylamide Gel Electrophoresis (PAGE)

DOI: 10.4236/ajps.2013.412A3001    3,128 Downloads   4,799 Views   Citations


Inositol phosphates are essential for cell development and signaling in all living organisms. Inositol hexakisphosphate (InsP6) is the most abundant phosphoinositol in both plants and animals. While the concentration of inorganic phosphorous (Pi) is often limited in soil, some plants overcome this limitation by creating a phosphate reservoir that serves as a source of Pi during phosphate deficiency. Although this strategy benefits plant development and signaling under adverse environmental conditions, excessive accumulation of Pi in crop plants has raised serious concerns about its toxicity and ill effects on human health. Consumption of crop plants with high InsP6 content or food products made from these crops is found to reduce nutrient intake significantly by way of chelating essential metal cations in human and livestock fed by such plants. Therefore, it is necessary to determine InsP6 contents in crop plants. Several methods have been developed for the screening and detection of InsP6 in plants. These detection methods however, are complex, labor-intensive, and often provide inaccurate results. We have developed a fast, reliable, and cost-effective method for the detection and quantification of InsP6 in plants using polyacrylamide gel electrophoresis (PAGE) with potential applications in industry, quality control labs, and research projects.

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The authors declare no conflicts of interest.

Cite this paper

M. Alimohammadi, N. Ali and M. Khodakovskaya, "Quantitative Detection of Inositol Hexakisphosphate (InsP6) in Crop Plants Using Polyacrylamide Gel Electrophoresis (PAGE)," American Journal of Plant Sciences, Vol. 4 No. 12C, 2013, pp. 1-6. doi: 10.4236/ajps.2013.412A3001.


[1] D. Oberleas, “Phytates,” In: Toxicants Occurring Naturally in Foods, 2nd Edition, National Academy of Sciences, Washington DC, 1973, pp. 363-371.
[2] F. A. Loewus, “Structure and Occurrence of Inositol in Plants,” In: D. J. Morri, W. F. Boss and F. A. Loewus, Eds., Inositol Metabolism in Plants, Wiley-Liss Inc., New York, 1990, pp. 1-11.
[3] V. Raboy, “Biochemistry and Genetics of Phytic Acid Synthesis,” In: D. J. Morré, W. F. Boss and F. A. Loewus, Eds., Inositol Metabolism in Plants, Wiley-Liss Inc., New York, 1990, pp. 55-76.
[4] J. N. A. Lott, “Accumulation of Seed Reserves of Phosphorus and Other Minerals,” In: D. R. Murray, Ed., Seed Physiology, Academic Press, 1984, pp. 139-166.
[5] J. N. A. Lott, J. S. Greenwood and G. D. Batten, “Mechanisms and Regulation of Mineral Nutrient Storage during Seed Development,” In: J. Kigel and G. Galili, Eds., Seed Development and Germination, Marcel Dekker, New York, 1995, pp. 215-235.
[6] J. Stevenson-Paulik, R. J. Bastidas, S. T. Chiou, R. A. Frye and J. D. York, “Generation of Phytate-Free Seeds in Arabidopsis through Disruption of Inositol Polyphosphate Kinases,” Proceedings of the National Academy of Sciences, Vol. 102, No. 35, 2005, pp. 12612-12617.
[7] J. S. Greenwood and J. D. Bewley, “Subcellular Distribution of Phytin in the Endosperm of Developing Castor Bean: A Possibility for Its Synthesis in the Cytoplasm Prior to Deposition within Protein Bodies,” Planta, Vol. 160, No. 2, 1984, pp. 113-120.
[8] L. R. Stephens and R. F. Irvine, “Stepwise Phosphorylation of Myo-Inositol Leading to Myo-Inositol Hexakisphosphate in Dictyostelium,” Nature, Vol. 346, No. 6284, 1990, pp. 580-583.
[9] J. van der Kaay, J. Wesseling and P. J. van Haastert, “Nucleus-Associated Phosphorylation of Ins(1,4,5)P3 to InsP6 in Dictyostelium,” Biochemical Journal, Vol. 312, No. 3, 1995, pp. 911-917.
[10] M. Singh and A. D. Krikorian, “Inhibition of Trypsin Activity in Vitro by Phytate,” Journal of Agricultural and Food Chemistry, Vol. 30, No. 4, 1982, pp. 799-800.
[11] S. R. Tannenbaum and V. R. Young, “Vitamins and Minerals,” In: O. R. Fennema, Ed., Food Chemistry, 2nd Edition, Marcel Dekker, New York, 1985.
[12] O. Losito, Z. Szijgyarto, A. C. Resnick and A. Saiardi, “Inositol Pyrophosphates and Their Unique Metabolic Complexity: Analysis by Gel Electrophoresis,” PLoS One, Vol. 4, No. 5, 2009, p. e5580.
[13] H. Marschner, “Mineral Nutrition of Higher Plants,” 2nd Edition, Academic Press, London, 1995.
[14] T. J. Chiou and S. I. Lin, “Signaling Network in Sensing Phosphate Availability in Plants,” Annual Review of Plant Biology, Vol. 62, No. 1, 2011, pp. 185-206.
[15] J. P. Singh, K. Selvendiran, M. Banu, R. Padmavathia and D. Sakthisekaran, “Protective Role of Apigenin on the Status of Lipid Peroxidation and Antioxidant Defense against Hepatocarcinogenesis in Wistar Albino Rats,” Phytomedicine, Vol. 11, No. 4, 2004, pp. 309-314.
[16] A. M. Shamsuddin, G. Y. Yang and I. Vucenik, “Novel Anti-Cancer Functions of IP6; Growth Inhibition and Differentiation of Human Mammary Cancer Cell Lines in Vitro,” Anticancer Research, Vol. 16, No. 6A, 1996, pp. 3287-3292.
[17] M. Hirose, S. Fukushima, K. Imaida, N. Ito and T. Shirai, “Modifying Effects of Phytic Acid and G-Oryzanol on the Promotion Stage of Rat,” Carcinogenesis, Vol. 19, 1999, pp. 3665-3670.
[18] R. Agarwal, H. Mumtaz and N. Ali, “Role of Inositol Polyphosphates in Programmed Cell Death,” Molecular and Cellular Biochemistry, Vol. 328, No. 1-2, 2009, pp. 155-165.
[19] L. R. McDowell, “Minerals in Animal and Human Nutrition,” Academic Press, San Diego, 1992.
[20] M. Gillooly, T. H. Bothwell, J. D. Torrance, A. P. MacPhail, D. P. Derman, W. R. Bezwoda, W. Mills, R. W. Charlton and F. Mayet, “The Effects of Organic Acids, Phytates and Polyphenols on the Absorption of Iron from Vegetables,” British Journal of Nutrition, Vol. 49 No. 3, 1983, pp. 331-342.
[21] P. R. Heaney, C. M. Weaver and M. L. Fitzsimmons, “Soybean Phytate Content: Effect on Calcium Absorption,” American Journal of Clinical Nutrition, Vol. 53, No. 3, 1991, pp. 745-747.
[22] K. Johansen and H. D. Poulsen, “Substitution of Inorganic Phosphorus in Pig Diets by Microbial Phytase Supplementation—A Review,” Pig News and Information, Vol. 24, No. 3, 2003, pp. 77N-82N.
[23] L. Barrientos, J. J. Scott and P. P. N. Murthy, “Specificity of Hydrolysis of Phytic Acid by Alkaline Phytase from Lily Pollen,” Plant Physiology, Vol. 106, No. 4, 1994, pp. 1489-1495.

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