Share This Article:

Morphology observation of dipalmitoyl phosphatidyl choline (DPPC) monolayer on water surface by dropping method

Abstract Full-Text HTML XML Download Download as PDF (Size:2771KB) PP. 115-121
DOI: 10.4236/jbpc.2013.44016    2,934 Downloads   4,232 Views   Citations


Preparation of DPPC lipid monolayer in water trough has been done by dropping method and compared with compression method. Monolayer was studied by surface pressure isotherm, fluorescence microscopy, Brewster angle microscopy, and infrared external reflection spectroscopy. Results of these measurements showed that dropping method gave better results compared to compression method. In dropping method, transition from liquid expanded state to liquid condensed is gradual compared to sharp one in compressed method. During monolayer formation, adjustment and interaction between hydrophilic part of lipid and water and among hydrophobic part of lipid molecule are slow, stable, and more natural as worked out from surface area versus pressure isotherm. At a given molecular area, surface pressure is less compared to compression method thus monolayer is in more fluidic state in dropping method than compression method. The observation was supported by all techniques described above.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Yoshida, D. , Yokoyama, T. , Shimoaki, T. , Tomita, T. , Yoshida, T. , Yamamoto, Y. , Taga, K. , Sumino, A. , Dewa, T. , Nango, M. , Yamamoto, M. and Sheravani, Z. (2013) Morphology observation of dipalmitoyl phosphatidyl choline (DPPC) monolayer on water surface by dropping method. Journal of Biophysical Chemistry, 4, 115-121. doi: 10.4236/jbpc.2013.44016.


[1] Singer, S.J. and Nicolson G.L. (1972) The fluid mosaic model of the structure of cell membranes. Science, 175, 720-731.
[2] Gennis, R.G. (1990) Biomembrane, molecular structure and function. Springer, New York.
[3] Ohnishi, S. (1993) Seitaimaku no doutekikouzou. 2nd Edition, University Tokyo Press, Tokyo.
[4] Takahashi, H., Yasue, T., Ohki, K. and Hatta, I. (1996) Structure and phase behavior of dimyristoylphosphatidic acid/poly(L-lysine) systems. Molecular Membrane Biology, 13, 233-240.
[5] Shimabayashi, S., terada, H. and Okabayashi, H. (1990) Seitai Colloid, Hirokawa Pub. Co., Tokyo.
[6] Ohe, C., Ida, Y., Matsumoto, S., Sakaki, T., Goto, Y., Noi, M., Tsurumaru, T. and Itoh, K. (2004) Investigations of polymyxin B-phospholipid interactions by vibrational sum frequency generation spectroscopy. The Journal of Physical Chemistry B, 108, 18081-18087.
[7] Ebara, Y. and Okahata, Y. (1994) A kinetic study of con-canavalin A binding to glycolipid monolayers by using a quartz-crystal microbalance. Journal of the American Chemical Society, 116, 11209-11212.
[8] Sato, T., Serizawa, T., Ohtake, F., Nakamura, M., Terabayashi, T., Kawanishi, Y. and Okahata, Y. (1998) Quantitative measurements of the interaction between mono-sialoganglioside monolayers and wheat germ agglutinin (WGA) by a quartz-crystal microbalance. Biochimica et Biophysica Acta, 1380, 82-92.
[9] Okahata, Y. and Furusawa, H. (2004) Gravimetry of biomolecules at the water-substrate interface: Quartz-crystal microbalance. Hyoumen Kagaku, 25, 131-138.
[10] Myers, D. (1991) Surfaces, interfaces, and colloids. VCH, Weinheim.
[11] Nagayama, K. (2000) Mizu to seimei. Kyoritsu Publication, Tokyo.
[12] Makino, M. and Kamiya, M. (1996) Effects of local an- esthetics on the dynamic behavior of phospholipid thin film. Langmuir, 12, 4221-4217.
[13] Kato, T., Matsumoto, N., Kawano, M., Suzuki, N. and Iriyama, K. (1994) Transition to three-dimensional structures in monolayers of a series of long-chain acids on the water surface by compression studied by transmission electron microscopy and atomic force microscopy. Thin Solid Films, 242, 223-228.
[14] Yamamoto, M., Suzuki, M., Kimura, T. and Itoh, K. (2008) Molecular structures at free surfaces of liquid nheptadecane investigated by infrared external reflection spectroscopy. The Journal of Physical Chemistry C, 112, 13232-13239.
[15] Mingotaud, A.F., Mingotaud, C. and Patterson, L.K. (1993) Handbook of monolayers, Vol. 1. Academic Press, San Diego.
[16] Yoshida, T., Koga, Y., Minowa, H., Kamaya, H. and Ueda, I. (2000) Interfacial lateral electrical conductance on lipid monolayer: Dose-dependent converse effect of alcohols. Journal of the American Chemical Society, 104, 1249-1252.
[17] Burn, A., Brezesinsuki, G., Mohwald, H., Blanzat, M., Perez, E. and Rico-Lattes, I. (2003) Interaction between phospholipids and new Gemini catanionic surfactants having anti-HIV activity. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 228, 3-16.
[18] M. Yamamoto, D. Yoshida, T. Yokoyama, H. Mori, K. Sekiguchi, Y. Yamamoto and K. Taga, “DPPC Langmuir Monolayer Prepared by Dropping Method: Probing the Tail Groups by Infrared External Reflection Spectroscopy.”
[19] Hatta, I. and Murata, M. (2000) Seitaimaku no dainamikusu. Kyouritsu Publication, Tokyo.
[20] Gauger, D.R., Selle, C., Fritzsche, H. and Pohle, W. (2001) Chain-length dependence of the hydration properties of saturated phosphatidylcholines as revealed by FTIR spectroscopy. Journal of Molecular Structure, 565-566, 25-29.

comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.