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Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization

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DOI: 10.4236/wjcd.2013.38080    3,518 Downloads   4,900 Views   Citations

ABSTRACT

The Amazon region is considered to be a high endemic area for Hepatitis B Virus (HBV) infections, Rondônia state having the highest prevalence. The aim of this study was to identify molecular genotypes and mutations in the S gene region of HBV viral genomes from 20 patients using a DNA microarray. Results: Serological tests showed that 88% of patients were HBeAg negative, 82% had anti-HBe antibodies and 33% were co-infected with Hepatitis Delta Virus. Sixteen percent of the patients were considered cirrhotic, and 11% have been transfused. The microarray technique identified the genotypes A (4 patients), D (7 patients) and F (7 patients) in 18 samples. Mutations were detected in all 3 genotypes and, overall, A159G, which has been associated with a reduced antigenicity of the virus, was detected most frequently. In genotype A, G119E was the most frequently detected mutation followed by mutations A159G, F134Y, W172C, Y161F and T143S. A159G was detected in all genotype D and F samples followed by mutations T143S, Y161F, N131T, T114S and G119E in genotype D and mutations T143S, Y161F, N131T, T114S and G119E in genotype F. Conclusion: The analysis of mutations repartition among genotypes suggests that some of them are preferentially or exclusively associated with genotype A, D or F. This type of tool is adapted for clinical and therapy monitoring of patient as well as for molecular epidemiology research on HBV.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Vieira, D. , Gauthier, M. , de Figueiredo Nicolete, L. , Santos, A. , Picelli, C. , Honda, E. , Paranhos-Baccalà, G. , Vernet, G. and Salcedo, J. (2013) Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization. World Journal of Cardiovascular Diseases, 3, 506-511. doi: 10.4236/wjcd.2013.38080.

References

[1] Lavanchy, D. (2004) Hepatitis B virus epidemiology, disease burden, treatment, and current and emerging prevention and control measures. Journal of Viral Hepatitis, 11, 97-107.
http://dx.doi.org/10.1046/j.1365-2893.2003.00487.x
[2] Gauthier, M., Bonnaud, B., Arsac, M., Lavocat, F., Maisetti, J., Kay, A., et al. (2010) Microarray for hepatitis B virus genotyping and detection of 994 mutations along the genome. Journal of Clinical Microbiology, 48, 4207-4215. http://dx.doi.org/10.1128/JCM.00344-10
[3] Sticchi, L., Caligiuri, P., Cacciani, R., Alicino, C. and Bruzzone, B. (2013) Epidemiology of HBV S-gene mutants in the Liguria Region, Italy: Implications for surveillance and detection of new escape variants. Human Vaccines & Immunotherapeutics, 9, 568-571.
http://dx.doi.org/10.4161/hv.23236
[4] Kao, J.-H., Liu, C.-J. and Chen, D.-S. (2002) Hepatitis B viral genotypes and lamivudine resistance. Journal of Hepatology, 36, 303-304.
http://dx.doi.org/10.1016/S0168-8278(01)00246-X
[5] Lai, C.L., Ratziu, V., Yuen, M.-F. and Poynard, T. (2003) Viral hepatitis B. The Lancet, 362, 2089-2094.
http://dx.doi.org/10.1016/S0140-6736(03)15108-2
[6] Schaefer, S. (2007) Hepatitis B virus taxonomy and hepatitis B virus genotypes. World Journal of Gastroenterology, 13, 14-21.
[7] Datta, S., Banerjee, A., Chandra, P.K., Chowdhury, A. and Chakravarty, R. (2006) Genotype, phylogenetic analysis, and transmission pattern of occult hepatitis B virus (HBV) infection in families of asymptomatic HBsAg carriers. Journal of Medical Virology, 78, 53-59.
http://dx.doi.org/10.1002/jmv.20503
[8] Weber, B. (2005) Genetic variability of the S gene of hepatitis B virus: clinical and diagnostic impact. Journal of Clinical Virology: The Official Publication of the Pan American Society for Clinical Virology, 32, 102-112.
http://dx.doi.org/10.1016/j.jcv.2004.10.008
[9] Francois, G., Kew, M., Damme, Van P., Mphahlele, M.J. and Meheus, A. (2001) Mutant hepatitis B viruses: A matter of academic interest only or a problem with farreaching implications? Vaccine, 19, 3799-3815.
http://dx.doi.org/10.1016/S0264-410X(01)00108-6
[10] Norder, H., Couroucé, A.-M., Coursaget, P., Echevarria, J.M., Lee, S.-D., Mushahwar, I.K., et al. (2004) Genetic diversity of hepatitis B virus strains derived worldwide: Genotypes, subgenotypes, and HBsAg subtypes. Intervirology, 47, 289-309. http://dx.doi.org/10.1159/000080872
[11] Mello, F.C.A., Souto, F.J.D., Nabuco, L.C., Villela-Nogueira, C.A., Coelho, H.S.M., Franz, H.C.F., et al. (2007) Hepatitis B virus genotypes circulating in Brazil: Molecular characterization of genotype F isolates. BMC Microbiology, 7, 103.
http://dx.doi.org/10.1186/1471-2180-7-103
[12] Ferreira, C.T. and Silveira, T.R. (2004) Hepatites virais: Aspectos da epidemiologia e da prevencao. Revista Brasileira de Epidemiologia, 7, 473-487.
http://dx.doi.org/10.1590/S1415-790X2004000400010
[13] Katsuragawa, T.H., Cunha, R.P.A., Salcedo, J.M.V., Souza, D.C.A., Oliveira, K.R.V., Gil, L.H.S., et al. (2010) Alta soroprevalência de infeccao pelos vírus das hepatites B e C na regiao do alto rio Madeira, Porto Velho, Rondonia, Brasil. Revista Pan-Amazonica de Saúde, 1, 91-106.
http://dx.doi.org/10.5123/S2176-62232010000200011
[14] Tran, N., Berne, R., Chann, R., Gauthier, M., Martin, D., Armand, M.-A., et al. (2006) European multicenter evaluation of high-density DNA probe arrays for detection of hepatitis B virus resistance mutations and identification of genotypes. Journal of Clinical Microbiology, 44, 2792-2800. http://dx.doi.org/10.1128/JCM.00295-06
[15] Ferreira, R.C., Teles, S.A., Dias, M.A., Tavares, V.R., Silva, S.A., Gomes, S.A., et al. (2006) Hepatitis B virus infection profile in hemodialysis patients in Central Brazil: prevalence, risk factors, and genotypes. Memórias Do Instituto Oswaldo Cruz, 101, 689-692.
http://dx.doi.org/10.1590/S0074-02762006000600019
[16] Oliveira, L.C.M. and Pontes, J.P.J. (2010) Frequency of hepatitis B immunity and occupational exposures to body fluids among Brazilian medical students at a public university. Revista Do Instituto de Medicina Tropical de Sao Paulo no date, 52, 247-252.
[17] Moraes, M.T., Niel, C. and Gomes, S.A. (1999) A polymerase chain reaction-based assay to identify genotype F of hepatitis B virus. Brazilian Journal of Medical and Biological Research, 32, 45-49.
[18] Araujo, N.M., Mello, F.C.A., Yoshida, C.F.T., Niel, C. and Gomes, S.A. (2004) High proportion of subgroup A’ (genotype A) among Brazilian isolates of Hepatitis B virus. Archives of Virology, 149, 1383-1395.
http://dx.doi.org/10.1007/s00705-003-0269-4
[19] Kay, A. and Zoulim, F. (2007) Hepatitis B virus genetic variability and evolution. Virus Research, 127, 164-176.
http://dx.doi.org/10.1016/j.virusres.2007.02.021
[20] Schaefer, S. (2005) Hepatitis B virus: Significance of genotypes. Journal of Viral Hepatitis, 12, 111-124.
http://dx.doi.org/10.1111/j.1365-2893.2005.00584.x
[21] Wu, C., Zhang, X., Tian, Y., Song, J., Yang, D., Roggendorf, M., et al. (2010) Biological significance of amino acid substitutions in hepatitis B surface antigen (HBsAg) for glycosylation, secretion, antigenicity and immunogenicity of HBsAg and hepatitis B virus replication. The Journal of General Virology, 91, 483-492.
http://dx.doi.org/10.1099/vir.0.012740-0
[22] Ruiz-Tachiquín, M.-E., Valdez-Salazar, H.-A., JuárezBarreto, V., Dehesa-Violante, M., Torres, J., Munoz-Hernández, O., et al. (2007) Molecular analysis of hepatitis B virus “a” determinant in asymptomatic and symptomatic Mexican carriers. Virology Journal, 4, 6.
http://dx.doi.org/10.1186/1743-422X-4-6
[23] Khan, N., Guarnieri, M., Ahn, S.H., Li, J., Zhou, Y., Bang, G., et al. (2004) Modulation of hepatitis B virus secretion by naturally occurring mutations in the S gene. Journal of Virology, 78, 3262-3270.
http://dx.doi.org/10.1128/JVI.78.7.3262-3270.2004
[24] Tong, S., Kim, K.-H., Chante, C., Wands, J. and Li, J. (2005) Hepatitis B Virus e Antigen Variants. International Journal of Medical Sciences, 2, 2-7.
http://dx.doi.org/10.7150/ijms.2.2
[25] Araujo, N.M., Vianna, C.O.A., Soares, C.C. and Gomes, S.A. (2008) A unique amino acid substitution, L215Q, in the hepatitis B virus small envelope protein of a genotype F isolate that inhibits secretion of hepatitis B virus subviral particles. Intervirology, 51, 81-86.
http://dx.doi.org/10.1159/000127430
[26] Oon, C.J., Lim, G.K., Ye, Z., Goh, K.T., Tan, K.L., Yo, S.L., et al. (1995) Molecular epidemiology of hepatitis B virus vaccine variants in Singapore. Vaccine, 13, 699-702.
http://dx.doi.org/10.1016/0264-410X(94)00080-7
[27] Ito, K., Qin, Y., Guarnieri, M., Garcia, T., Kwei, K., Mizokami, M., et al. (2005) Impairment of Hepatitis B Virus Virion Secretion by Single-Amino-Acid Substitutions in the Small Envelope Protein and Rescue by a Novel Glycosylation Site. Journal of Virology, 79, 14404-14410.

  
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