Share This Article:

Search for a Microsatellite Marker Linked with Resistance Gene to Xanthomonas axonopodis pv. malvacearum in Brazilian Cotton

Abstract Full-Text HTML Download Download as PDF (Size:244KB) PP. 2039-2042
DOI: 10.4236/ajps.2013.410255    3,569 Downloads   4,903 Views   Citations

ABSTRACT

The cotton cultivar DELTAOPAL is resistant under field as well as under glasshouse conditions to the Brazilian isolates of Xanthomonas axonopodis pv. malvacearum (Xam). Segregating populations derived from the cross between this cultivar and one susceptible cv. BRS ITA 90, were utilized to identify molecular marker linked with the resistance gene to Xam by “Bulk Segregant Analysis (BSA)”. Two hundred and twenty microsatellite (Single Sequence Repeat—SSR) primers were tested. The amplification products were visualized in polyacrylamide gels stained with silver nitrate. Only one primer was informative and showed polymorphism between the DNA of the parents and their respective bulks of homozygous F2 populations contrasting for resistance and susceptibility, and hence was used to analyze DNA of 120 F2 populations. The microsatellite primer yielded one band of 80 bp linked with the resistance locus, which was absent in the susceptible parent as well as in the bulk of the homozygous susceptible plants of the cross. The segregation ratio as determined by phenotypic analysis was 3R:1S. It is believed that the microsatellite marker was linked with the resistance locus and hence may offer new perspectives for marker assisted selection against the angular leaf spot disease of cotton. It is however, felt necessary to repeat the microsatellite analysis and make sure that the primer is tightly linked with the resistance locus and at the same time verify the genetic distance between the marker and the resistance locus.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

M. Marangoni, L. Girotto, M. Nunes, W. Almeida, R. Galbieri, I. Schuster and Y. Mehta, "Search for a Microsatellite Marker Linked with Resistance Gene to Xanthomonas axonopodis pv. malvacearum in Brazilian Cotton," American Journal of Plant Sciences, Vol. 4 No. 10, 2013, pp. 2039-2042. doi: 10.4236/ajps.2013.410255.

References

[1] T. P. Wallace and K. M. El-Zik, “Inheritance of Resistance in Three Cotton Cultivars to the HV1 Isolate of Bacterial Blight,” Crop Science, Vol. 29, No. 5, 1989, pp. 1114-1119.
http://dx.doi.org/10.2135/cropsci1989.0011183X002900050003x
[2] Y. R. Mehta, “A Semi-Selective Agar Medium to Detect the Presence of Xanthomonas axonopodis pv. Malvacearum in Naturaly Infected Cotton Seed,” Fitopatologia Brasileira, Vol. 30, No. 5, 2005, pp. 489-496.
http://dx.doi.org/10.1590/S0100-41582005000500005
[3] C. Zandoná, Y. R. Mehta, I. Schuster, P. F. R. Alves, C. A. Bomfeti, K. R. P. Bibanco, R. B. Silva, L. P. Lopes, “Mecanismo Genético de Resistência em três Cultivares de Algodoeiro a Xanthomonas axonopodis pv. Malvacearum,” Fitopatologia Brasileira, Vol. 30, No. 6, 2005.
[4] J. E. Hamer, “Molecular Probes for Rice Blast Disease,” Science, Vol. 252, No. 5006, 1991, pp. 632-633.
http://dx.doi.org/10.1126/science.252.5006.632
[5] A. L. Alzate-Martin, H. Metarim, G. A. Carvalho, T. J. Paula Jr., E. G. Barros and M. A. Morreira, “Improved Selection with Newly Identified RAPD Markers Linked to Resistance Gene to Four Pathotypes of Colletotrichum lindemuthianum in Common Bean,” Physiopathology, Vol. 89, No. 4, 1999, pp. 281-285.
http://dx.doi.org/10.1094/PHYTO.1999.89.4.281
[6] L. G. Araujo, A. S. Prabhu and M. C. Fillipi, “Identification of RAPD Marker Linked to Blast Resistance Gene in a Somaclone of Rice Cultivar Araguaia,” Fitopatologia Brasileira, Vol. 27, No. 2, 2002, pp. 181-185.
http://dx.doi.org/10.1590/S0100-41582002000200010
[7] G. Bai, F. L. Kolb, G. Shaner and L. Domer, “Amplified Fragment Length Polymorphism Markers Linked to a Major Quantitative Trait Locus Controlling Scab Resistance in Wheat,” Phytopathology, Vol. 89, No. 4, 1999, pp. 343-348.
http://dx.doi.org/10.1094/PHYTO.1999.89.4.343
[8] G. A. Carvalho, T. Sediyama, A. L. Alzate-Martin, E. J. Barros and M. A. Moreira, “Identificação de Marcadores RAPD Ligados a um Gene de Resistência ao Cancro da Haste da Soja,” Fitopatologia Brasileira, Vol. 27, No. 5, 2002, pp. 474-478.
http://dx.doi.org/10.1590/S0100-41582002000500006
[9] R. W. Michelmore, I. Paran and R. V. Resseli, “Identification of Markers Linked to Disease Resistance Genes by Bulked Segregant Analysis: A Rapid Method to Detect Markers in Specific Genomic Regions Using Segregating Populations,” Proceedings of the National Academy of Sciences, Vol. 88, No. 21, 1991, pp. 9828-9832.
http://dx.doi.org/10.1073/pnas.88.21.9828
[10] M. A. Saghai-Maroof, Y. G. Yue, Z. X. Xiang, E. L. Stromberg and G. K. Rufener, “Identification of Quantitative Trait Loci Controlling Resistance to Gray Leaf Spot Disease in Maize,” Theoretical and Applied Genetics, Vol. 93, No. 4, 1996, pp. 539-546.
http://dx.doi.org/10.1007/BF00417945
[11] R. J. Wright, P. M. Thaxton, A. H. Paterson and K. M. ElZik, “QTL Analysis of Bacterial Blight Resistance Genes in Cotton Using RFLP Markers,” Proceedings of the Beltwide Cotton Conference, National Cotton Council, Memphis, Vol. 1, 1996, pp. 635-636.
[12] N. D. Young, “A Cautiously Optimistic Vision for MarkerAssisted Breeding,” Molecular Breeding, Vol. 5, No. 6, 1999, pp. 505-510.
http://dx.doi.org/10.1023/A:1009684409326
[13] Y. R. Mehta, C. Bomfeti and V. Bolognini, “A Semiselective Agar Medium to Detect the Presence of Xanthomonas axonopodis pv. malvacearum in Naturally Infected Cotton Seed,” Fitopatologia Brasileira, Vol. 30, No. 5, 2004, pp. 489-496.
http://dx.doi.org/10.1590/S0100-41582005000500005
[14] J. J. Doyle and J. L. Doyle, “Isolation of Plant DNA from Fresh Tissue,” Focus, Vol. 12, 1990, pp. 13-15.
[15] J. D. Kelly, “Use of Random Amplified Polymorphic DNA Markers in Breeding for Major Gene Resistance to Plant Pathogens,” Horticultural Science, Vol. 30, 1995, pp. 461-465.

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.