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Adsorption of α-Chymotrypsin on Plant Biomass Charcoal

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DOI: 10.4236/jsemat.2013.34036    2,742 Downloads   4,194 Views   Citations


The adsorption of α-chymotrypsin onto plant biomass charcoal (PBC), which was prepared from plant biomass wastes such as bagasse and dumped adzuki beans by pyrolysis, has been examined. The PBC was characterized by SEM, specific surface area, and pore size distribution. The adsorption isotherms were successfully correlated by the Freundlich equation. The amount of α-chymotrypsin adsorbed on PBC was dramatically dependent upon the solution pH and temperature. Maximum adsorptions of α-chymotrypsin on adzuki bean charcoal and bagasse charcoal were observed at weak acidic and near neutral pH, respectively. The amount of α-chymotrypsin adsorbed on PBC decreased with an increase in the concentration of salts. Plots of the amount of α-chymotrypsin adsorbed on PBC versus temperature exhibited an optimum temperature.

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Cite this paper

H. Noritomi, K. Hishinuma, S. Kurihara, J. Nishigami, T. Takemoto, N. Endo and S. Kato, "Adsorption of α-Chymotrypsin on Plant Biomass Charcoal," Journal of Surface Engineered Materials and Advanced Technology, Vol. 3 No. 4, 2013, pp. 269-274. doi: 10.4236/jsemat.2013.34036.


[1] A. Cross and S. P. Sohi, “The Priming Potential of Biochar Products in Relation to Labile Carbon Contents and Soil Organic Matter Status,” Soil Biology & Biochemistry, Vol. 43, No. 10, 2011, pp. 2127-2134.
[2] L. L. Pulido, T. Hata, Y. Imamura, S. Ishihara and T. Kajimoto, “Removal of Mercury and Other Metals by Carbonized Wood Powder from Aqueous Solutions of their Salts,” Journal of Wood Science, Vol. 44, No. 3, 1998, pp. 237-243.
[3] I. Abe, M. Hitomi, N. Ikuta, I. Kawafune, K. Noda and Y. Kera, “Humidity-Control Capacity of Microporous Carbon,” Seikatsu Eisei, Vol. 39, No. 6, 1995, pp. 333-336.
[4] B. Khalfaoui, A. H. Meniai and R. Borja, “Removal of Copper from Industrial Wastewater by Raw Charcoal Obtained from Reeds,” Journal of Chemical Technology and Biotechnology, Vol. 64, No. 2, 1995, pp. 153-156.
[5] M. Yatagai, R. Ito, T. Ohira and K. Oba, “Effect of Charcoal on Purification of Wastewater,” Mokuzai Gakkaishi, Vol. 41, No. 4, 1995, pp. 425-432.
[6] H. Kominami, K. Sawai, M. Hitomi, I. Abe and Y. Kera, “Reduction of Nitrogen Monoxide by Charcoal,” Nippon Kagakukaishi, No. 6, 1994, pp. 582-584.
[7] C. Haynes and W. Norde, “Structure and Stabilities of Adsorbed Proteins,” Journal of Colloid and Interface Science, Vol. 169, No. 2, 1995, pp. 313-328.
[8] K. Nakanishi, T. Sakiyama and K. Imamura, “On the Adsorption of Proteins on Solid Surfaces, a Common but Very Complicated Phenomenon,” Journal of Bioscience and Bioengineering, Vol. 91, No. 4, 2001, pp. 233-244.
[9] H. Noritomi, D. Iwai, R. Kai, M. Tanaka and S. Kato, “Adsorption of Lysozyme on Biomass Charcoal Powder Prepared from Plant Biomass Wastes,” Journal of Chemical Engineering of Japan, Vol. 46, No. 3, 2013, pp. 196-200.
[10] H. Noritomi, R. Ishiyama, R. Kai, D. Iwai, M. Tanaka and S. Kato, “Immobilization of Lysozyme on Biomass Charcoal Powder Derived from Plant Biomass Wastes,” Journal of Biomaterials and Nanobiotechnology, Vol. 3, No. 3, 2012, pp. 446-451.
[11] H. Noritomi, R. Kai, D. Iwai, H. Tanaka, R. Kamiya, M. Tanaka, K. Muneki and S. Kato, “Increase in Thermal Stability of Proteins Adsorbed on Biomass Charcoal Powder Prepared from Plant Biomass Wastes,” Journal of Biomedical Science and Engineering, Vol. 4, No. 11, 2011, pp. 692-698.
[12] A. Kumar and P. Venkatesu, “Overview of the Stability of α-Chymotrypsin in Different Solvent Media,” Chemical Reviews, Vol. 112, No. 7, 2012, pp. 4283-4307.
[13] T. Asada, S. Ishihara, T. Yamane, A. Toba, A. Yamada and K. Oikawa, “Science of Bamboo Charcoal: Study on Carbonizing Temperature of Bamboo Charcoal and Removal Capability of Harmful Gases,” Journal of Health Science, Vol. 48, No. 6, 2002, pp. 473-479.
[14] E. P. Barrett, L. G. Joyner and P. H. Halenda, “The Determination of Pore Volume and Area Distributions in Porous Substances. I. Computations from Nitrogen Isotherms,” Journal of the American Chemical Society, Vol. 73, No. 1, 1951, pp. 373-380.
[15] W. Adamson, “Physical Chemistry of Surfaces,” 4th Edition, John Wiley & Sons, New York, 1982, p. 373.
[16] K. Nishimiya, T. Hata, Y. Imamura and S. Ishihara, “Analysis of Chemical Structure of Wood Charcoal by X-Ray Photoelectron Spectroscopy,” Journal of Wood Science, Vol. 44, No. 1, 1998, pp. 56-61.
[17] W. Norde and J. Lyklema, “The Adsorption of Human Plasma Albumin and Bovine Pancreas Ribonuclease at Negatively Charged Polystyrene Surfaces,” Journal of Colloid and Interface Science, Vol. 66, No. 2, 1978, pp. 257-265.

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