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Physicochemical Characteristics and QTL Mapping Associated with the Lipid Content of High-Lipid Rice

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DOI: 10.4236/ajps.2013.410241    3,270 Downloads   4,683 Views   Citations

ABSTRACT

This study was conducted to examine the physicochemical characteristics and perform QTL mapping of genetic factors associated with the lipid content of rice. A rice strain with a high lipid content, P31-2-2-2-B-B, was developed from mutants of Dongjin created by T-DNA insertion. The lipid content of P31-2-2-2-B-B brown rice was 4.42% whereas that of the donor cultivar Dongjin was 2.56%. The total fatty acid content of the high-lipid mutant brown rice was 7.82% and that of Dongjin was 3.43%. The unsaturated fatty acid composition of the mutant brown rice was 2.73% oleic acid, 2.74% linoleic acid, and 0.34% linolenic acid. In contrast, the fatty acid composition of the donor cultivar Dongjinwas 1.30% oleic acid and 0.99% linoleic acid. The percentage of unsaturated fatty acid to total fatty acid in the high-lipid mutant was higher (74.3%) than that of Dongjin (66.8%). Continuous frequency distribution and transgressive segregation of the lipid content were observed in the F3 family (seeds) derived from a cross between the high-lipid mutant P31-2-2-2-B-Band a tongil-type cultivar Samgang. This result implied that the lipid content was a quantitative trait controlled by a polygene. Additionally, the broad sense heritability of lipid content was estimated to be 89.6% based on analysis of the F3 seeds. A significant QTL, qRLC5, was identified on chromosome 5 with a LOD score of 2.37, and was flanked by 5007 and 5014. Results of the present study should be useful for improving rice nutritional quality through marker-assisted selection.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

N. Kim, J. Sohn and K. Kim, "Physicochemical Characteristics and QTL Mapping Associated with the Lipid Content of High-Lipid Rice," American Journal of Plant Sciences, Vol. 4 No. 10, 2013, pp. 1949-1953. doi: 10.4236/ajps.2013.410241.

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