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Quantification of Organochlorine Pesticide Residues in the Buffalo Milk Samples of Delhi City, India

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DOI: 10.4236/jep.2013.49111    4,860 Downloads   7,891 Views   Citations


The ill effects of green revolution include residues of extensively used chemical pesticides in various environmental components. The present study was designed to analyze the levels of organochlorine pesticide residues along with chemical composition in buffalo milk samples collected from different localities of Delhi. Milk monitoring can yield information about the kinds and quantities of pesticides in the environment as well as in our daily diet. In this study, the residue of three different organochlorine pesticides, namely Hexachlorocyclohexane (HCH), Dichlorodiphenyltrichloroethane (DDT) and Endosulfan have been reported. Residues of Lindane exceeded the Maximum Residual Limit values in 50% of the samples is a cause of serious concern. The p,p-DDT was detected in 70% of the samples with p,p’-DDE (dichlorodiphenyldichloroethylene) in 80% of the milk samples of different parts of Delhi state. DDD (Dichlorodiphenyldichloroethane) another metabolite of p,p’-DDT was detected in 65% of the milk samples. The analysis indicates that DDT is the major contaminants in different parts of Delhi state. α and β endosulfan were detected in 35% and 40% of the samples analyzed. The statistical correlation shows no significant correlation between chemical compositions of the samples. The presence of multiple chemicals in virtually all samples of buffalo milk raises new questions about the possible toxicological impacts of chemical mixtures on an infant’s developing nervous and immune systems and reproductive organs.

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The authors declare no conflicts of interest.

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M. Aslam, S. Rais and M. Alam, "Quantification of Organochlorine Pesticide Residues in the Buffalo Milk Samples of Delhi City, India," Journal of Environmental Protection, Vol. 4 No. 9, 2013, pp. 964-974. doi: 10.4236/jep.2013.49111.


[1] K. Ikhtiar and Z. Alam, “Nutritional Composition of Pakistani Wheat Varieties,” Journal of Zhejiang University Science B, Vol. 8, No. 8, 2007, pp. 555-559. doi:10.1631/jzus.2007.B0555
[2] M. S. Baloch, I. U. Awan and G. Hassan, “Growth and Yield of Rice as Affected by Transplanting Dates and Seedlings per Hill under High Temperature of Dera Ismail Khan, Pakistan,” Journal of Zhejiang University Science B, Vol. 7, No. 7, 2006, pp. 572-579. doi:10.1631/jzus.2006.B0572
[3] S. Rahman, N. Khalid, J. H. Zaidi, S. Ahmad and M. Z. Iqbal, “Non-Occupational Lead Exposure and Hypertension in Pakistani Adults,” Journal of Zhejiang University Science B, Vol. 7, No. 9, 2006, pp. 732-737. doi:10.1631/jzus.2006.B0732
[4] N. Ribas-Fito, E. Cardo, M. Sala, M. E. de Muga, C. Mazon, A. Verdu, M. Kogevinas, O. J. Grimalt and J. Sunyer, “Breastfeeding, Exposure to Organochlorine Compounds and Neurodevelopment in Infants,” Pediatrics, Vol. 111, No. 5, 2003, pp. 580-585. doi:10.1542/peds.111.5.e580
[5] M. A. Dalvie, J. E. Myers, M. L. Thompson, T. G. Robins, S. Dyer, J. Riebow, J. Molekwa, M. Jeebhay, R. Millar and P. Kruger, “The Long-Term Effects of DDT Exposure on Semen, Fertility, and Sexual Function of Malaria Vectorcontrol Workers in Limpopo Province,” South African Environmental Research, Vol. 96, No. 1, 2004, pp. 1-8. doi:10.1016/j.envres.2003.09.002
[6] M. P. Longnecker, M. A. Klebanoff, H. Zhou and J. W. Brock, “Association between Maternal Serum Concentration of the DDT Metabolite DDE and Preterm and Smallfor-Gestational-Age Babies at Birth,” The Lancet, Vol. 358, No. 9276, 2001, pp. 110-114. doi:10.1016/S0140-6736(01)05329-6
[7] G. S. Cooper, S. A. Martin, M. P. Longnecker, D. P. Sandler and D. R. Germolec, “Associations between Plasma DDE Levels and Immunologic Measures in African-American Farmers in North Carolina,” Environmental Health Perspectives, Vol. 112, No. 10, 2004, pp. 1080-1084. doi:10.1289/ehp.6892
[8] P. J. John, N. Bakore and P. Bhatnagar, “Assessment of Organochlorine Pesticide Residue Levels in Dairy Milk and Buffalo Milk from Jaipur City, Rajasthan, India,” Environment International, Vol. 26, 2001, pp. 231-236. doi:10.1016/S0160-4120(00)00111-2
[9] J. F. Davies, V. H. Freed and F. W. Whittemore, “An Agro Medical Approach to Pesticide Management,” University of Miami School of Medicine, Coral Gables, 1986.
[10] E. Kampire, B. T. Kiremire, S. A. Nyanzi and M. Kishimba, “Organochlorine Pesticide in Fresh and Pasteurized Cow’s Milk from Kampala Markets,” Chemosphere, Vol. 84, No. 7, 2011, pp. 923-927. doi:10.1016/j.chemosphere.2011.06.011
[11] A. C. Ribeiro and S. D. A. Ribeiro, “Specialty Products Made from Goat Milk,” Small Ruminant Research, Vol. 89, 2010, pp. 225-233. doi:10.1016/j.smallrumres.2009.12.048
[12] A. Alle, A. Dembelle, B. Yao and G. Ado, “Distribution of Organochlorine Pesticides in Human Breast Milk and Adipose Tissue from Two Locations in Cote d’Ivoire,” Asian Journal of Applied Sciences, Vol. 2, No. 5, 2009, pp. 456-462. doi:10.3923/ajaps.2009.456.463
[13] K. Borga, G. W. Gabrielsen and J. U. Skaare, “Biomagnification of Organochlorines along a Barents Sea Food Chain,” Environmental Pollution, Vol. 113, No. 2, 2001, pp. 187-198. doi:10.1016/S0269-7491(00)00171-8
[14] A. Di Muccio, I. Camoni, R. Dommarco, A. Santilio, A. Ausili, M. Rizzica, B. Gigli and C. Calzolari, “Evaluation of p,p′-DDE, p,p′-DDT and Polychlorobiphenyls (PCBs) Levels in Samples of Human Milk from Rome, Florence and the Surrounding Areas,” Annali dell'Istituto Superiore di Sanita, Vol. 26, No. 2, 1990, pp. 155-160.
[15] H. Tian, “Determination of Chloramphenicol, Enrofloxacin and 29 Pesticides Residues in Bovine Milk by Liquid Chromatography-Tandem Mass Spectrometry,” Chemosphere, Vol. 83, 2011, pp. 349-355. doi:10.1016/j.chemosphere.2010.12.016
[16] K. Mishra and R. C. Sharma, “Assessment of Organochlorine Pesticides in Human Milk and Risk Exposure to Infants from North-East India,” Science of the Total Environment, Vol. 409, 2011, pp. 4939-4949. doi:10.1016/j.scitotenv.2011.07.038
[17] H. Van den Berg, R. Velayudhan, A. Ebol, B. H. Catbagan, R. Turingan, M. Tuso and J. Hii, “Operational Efficiency and Sustainability of Vector Control of Malaria and Dengue: Descriptive Case Studies from the Philippines,” Malaria Journal, Vol. 11, 2012, pp. 269-279. doi:10.1186/1475-2875-11-269
[18] G. R. J. Khaniki, “Chemical Contaminants in Milk and Public Health Concerns: A Review,” International Journal of Dairy Science, Vol. 2, No. 2, 2007, pp. 104-115. doi:10.3923/ijds.2007.104.115
[19] E. Tsiplakou, C. J. Anagnostopoulos, K. Liapis, S. A. Haroutounian and G. Zervas, “Pesticides Residues in Milks and Feedstuff of Farm Animals Drawn from Greece,” Chemosphere, Vol. 80, No. 5, 2010, pp. 504-512. doi:10.1016/j.chemosphere.2010.04.069
[20] FAO/WHO, “Joint FAO/WHO Food Standards Programme-Codex Alimentarius Commission-Codex Alimentarius-Volume II. Pesticide Residues in Food,” Food and Agriculture Organization/World Health Organization, Rome, 1993.
[21] European Community, “Council Directive 93/57/EEC of 29 June 1993 Ammending the Annexes to Directives 86/362/EEC and 86/363/EEC on the Fixing of Maximum Levels for Pesticides Residues in and on Cereals and Foodstuffs of Animal Origin, Respectively,” Official Journal of the European Communities, Vol. 211, 1993, pp. 1-5.
[22] E. Nanu, C. Latha, B. Sunil, Prejit, M. Thomas and K. V. Menon, “Quality Assurance and Public Health Safety of Raw Milk at the Production Point,” American Journal of Food Technology, Vol. 2, No. 3, 2007, pp. 145-152. doi:10.3923/ajft.2007.145.152
[23] M. Fernandez-Alvarez, M. Llompart, J. P. Lamas, M. Lores, C. Garcia-Jares, R. Cela and T. Dagnac, “Development of a Solid-Phase Microextraction Gas Chromatography with Microelectron-Capture Detection Method for a Multiresidue Analysis of Pesticides in Bovine Milk,” Analytica Chimica Acta, Vol. 617, 2008, pp. 37-50. doi:10.1016/j.aca.2008.01.021
[24] S. M. Waliszewski, V. T. Pardio, K. N. Waliszewski, J. N. Chantiri, A. A. Aguirre, R. M. Infanzon and J. Rivera, “Organochlorine Pesticide Residues in Cow’s Milk and Butter in Mexico,” Science of the Total Environment, Vol. 208, No. 1-2, 1997, pp. 127-132. doi:10.1016/S0048-9697(97)00270-2
[25] R. K. Mishra, R. B. Dominguez, S. Bhand, R. Munoz and J. L. Marty, “A Novel Automated Flow-Based Biosensor for the Determination of Organophosphate Pesticides in Milk,” Biosensors and Bioelectronics, Vol. 32, No. 1, 2012, pp. 56-61. doi:10.1016/j.bios.2011.11.028
[26] R. D. Johnson and D. D. Manske, “Pesticides in Food and Feed. Pesticide and Other Chemical Residues in Total Diet Samples,” Pesticides Monitoring Journal, Vol. 11, 1977, pp. 116-131.
[27] D. Mukherjee, B. R. Roy, J. Chakraborty and B. N. Ghosh, “Pesticide Residues Inhuman Food in Calcutta,” Indian Journal of Medical Research, Vol. 72, 1980, pp. 577-582.
[28] K. Fytianos, G. Vasilikiotis, L. Weil, E. Kavlendis and N. Laskaridis, “Preliminary Study of Organochlorine Compounds in Milk Products, Human Milk and Vegetables,” Bulletin of Environmental Contamination and Toxicology, Vol. 34, No. 1, 1985, pp. 504-508. doi:10.1007/BF01609767
[29] P. K. Vaidya, K. C. Goyal and V. Singh, “Evaluation of Endosulfan Residue in Okra (Abelmaschus esculantus) Fruit,” Ad. Bios., Vol. 10, 1991, pp. 107-110.
[30] R. Kashyap, L. R. Iyer and M. M. Singh, “Evaluation of Daily Dietary Intake of Dichlorodiphenyltrichloroethane (DDT) and Benzene Hexachloride (BHC) in India,” Archives of Environmental Health, Vol. 49, 1994, pp. 63-66. doi:10.1080/00039896.1994.9934417
[31] R. S. Battu, B. Singh, K. K. Chahal and R. L. Kalra, “Contamination of Animal Feed with Residues of HCH and DDT,” Pest Research Journal, Vol. 8, 1996, pp. 172-175.
[32] N. G. Mitra, A. Upadhyaya, B. Sachidanand and G. D. Agrawal, “Contamination of Pesticides in Samples of Milk and Milk Products,” Pestology, Vol. 23, No. 8, 1999, pp. 36-40.
[33] G. Devanathan, A. Subramanian, M. Someya, A. Sudaryanto, T. Isobe, S. Takahashi, P. Chakraborty and S. Tanabe, “Persistent Organochlorines in Human Breast Milk from Major Metropolitan Cities in India,” Environmental Pollution, Vol. 157, No. 1, 2009, pp. 148-154. doi:10.1016/j.envpol.2008.07.011
[34] R. S. Battu, B. Singh and B. K. Kang, “Contamination of Liquid Milk and Butter with Pesticide Residues in the Ludhiana District of Punjab State, India,” Ecotoxicology and Environmental Safety, Vol. 59, No. 3, 2004, pp. 324-331. doi:10.1016/j.ecoenv.2003.08.017
[35] R. L. Kalra and R. P. Chawla, “Studies on Pesticides Residues and Monitoring of Pesticidal Pollution,” Final Technical Report, PL480 Project, Punjab, Agricultural University, Ludhiana, 1983.
[36] N. P. Agnihotri, R. S. Dewan, H. K. Jain and S. Y. Pandey, “Residues of Insecticides in Food Commodities from Delhi: II. High Fat Content Food Materials,” Indian Journal of Entomology, Vol. 36, No. 3, 1974, pp. 203-208.
[37] G. S. Dhaliwal and R. L. Kalra, “DDT Residues in Milk Samples from Ludhiana and Surrounding Areas,” Indian Journal of Entomology, Vol. 4, No. 1, 1977, pp. 13-22.
[38] M. C. Saxena and M. K. J. Siddiqui, “Pesticide Pollution in India: Organochlorine Pesticides in Milk of Woman, Buffalo, and Goat,” Journal of Dairy Science, Vol. 65, No. 3, 1982, pp. 430-434. doi:10.3168/jds.S0022-0302(82)82209-1
[39] T. S. Kathpal, G. Singh, G. S. Yadav, J. S. Dhankar and A. Singh, “Monitoring of Milk and Milk Products for DDT and HCH Contamination,” Pesticide Research Journal, Vol. 4, No. 2, 1992, pp. 123-131.
[40] N. R. Kumar and A. Nath, “Monitoring of Bovine Milk for DDT and HCH,” Pesticide Research Journal, Vol. 8, No. 1, 1996, pp. 90-92.
[41] U. K. Srivastava and N. T. Patel, “Pesticides Industry in India,” Oxford and IBH Publishing Company Ltd., New Delhi, 1990.
[42] AOAC, “Official Methods of the Association Official Analytical Chemists,” Washington DC, 1995.
[43] K. Helrich, “Official Methods of Analysis of the Association of Official Analytical Chemists,” 15th Edition, Association of Official Analytical Chemists, Inc., Arlington, 1990.
[44] H. P. Their and H. Zeumer, “DFG Method S16: Organophosphorus Pesticides with Thioether Groups,” In: H. P. Their and H. Zeumer, Eds., Manual of Pesticide Residue Analysis, Vol. 1, Verlag Chemie, New York, 1987, p. 309.
[45] M. C. Bowman and M. Beroza, “Determination of Fenthion and Five of Its Metabolites in Corn, Grass and Milk,” Journal of Agricultural and Food Chemistry, Vol. 16, No. 3, 1968, pp. 399-402. doi:10.1021/jf60157a005
[46] V. Leoni, A. M. Caricchia and S. Chiavarini, “Multiresidue Method for Quantitation of Organophosphorus Pesticides in Vegetable and Animal Foods,” Journal of Association of Official Analytical Chemists International, Vol. 75, No. 3, 1992, pp. 511-518.
[47] R. Frank, H. E. Braun, G. H. Sirons, J. Rasper and G. G. Ward, “Organochlorine and Organophosphorus Insecticides and Industrial Pollutants in the Milk Supplies of Ontario,” Journal of Food Protection, Vol. 48, No. 6, 1985, pp. 499-504.
[48] M. P. Carvalho, M. C. Duraes, C. E. Martins, F. Deresz, J. R. F. Brito, A. F. Freitas, J. A. B. Portugal and C. N. Costa, “Effect of Nutrition on Milk Quality and Composition,” Proceedings of the Anais do 2o Minas Leite: Avancos Tecnologicos Para Oaumento da Produtividade Leiteira, Juiz de Fora, May 2000, pp. 77-82.
[49] B. H. Webb, A. H. Johnson and J. A. Alford, “Fundamentals of Dairy Chemistry,” 3rd Edition, Chapman and Hall, London, 1974.
[50] M. Ayub, Q. Ahmad, M. Abbas, I. M. Qazi and I. A. Khattak, “Composition and Adulteration Analysis of Milk Samples,” Sarhad Journal of Agriculture, Vol. 23, No. 4, 2007, pp. 1127-1130.
[51] I. Q. El Beit, J. V. Wheelock and D. E. Cotton, “Factors Affecting Soil Residues of Dieldrin, Endosulfan, Gamma HCH, Dimethoate and Pyrolan,” Ecotoxicology and Environmental Safety, Vol. 5, No. 2, 1981, pp. 135-160. doi:10.1016/0147-6513(81)90030-0
[52] G. Vettorazzi, “State of the Toxicological Evaluation Carried Out by the Joint FAO/WHO Expert Committee on Pesticide Residues, I, Organohalogenated Pesticides Used in Public Health and Agriculture,” Residue Reviews, Vol. 56, 1975, pp. 107-134. doi:10.1007/978-1-4613-9388-7_3
[53] K. Pfeilsticker, “Pesticides in Children’s Diets,” Monthly Journal of Pediatrics, Vol. 121, 1973, pp. 551-553.
[54] F. W. Kutz, P. H. Wood and D. P. Bottimore, “Organochlorine Pesticides and Polychlorinated Biphenyls in Human Adipose Tissue,” Reviews of Environmental Contamination and Toxicology, Vol. 120, 1991, pp. 1-82. doi:10.1007/978-1-4612-3080-9_1
[55] A. Kumar, P. Dayal, G. Shukla, G. Singh and P. E. Joseph, “DDT and HCH Residue Load in Mother’s Breast Milk: A Survey of Lactating Mother’s from Remote Villages in Agra Region,” Environment International, Vol. 32, No. 2, 2006, pp. 248-251. doi:10.1016/j.envint.2005.08.019
[56] A. Ashnagar, N. N. Gharib and F. M. Cheraghi, “Determination of Organochlorine Pesticide Residues in Cow’s Milk Marketed in Ahwaz City of Iran,” International Journal of pharmTech Research, Vol. 1, No. 2, 2009, pp. 247-251.
[57] S. M. Waliszewski, V. T. Pardio, K. N. Waliszewski, J. N. Chantiri, R. M. Infanzon and J. Rivera, “Detection of Some Organochlorine Pesticides in Cow’s Milk,” Food Additives and Contaminants, Vol. 13, No. 2, 1996, pp. 231-235. doi:10.1080/02652039609374401
[58] K. Kannan, S. Tanabe, A. Ramesh, A. Subramanian and R. Tatsukawa, “Persistent Organochlorine Residues in Foodstuffs from India and Their Implications on Human Dietary Exposure,” Journal of Agricultural and Food Chemistry, Vol. 40, No. 3, 1992, pp. 518-524. doi:10.1021/jf00015a032
[59] S. K. Nag and M. K. Raikwar, “Organochlorine Pesticide Residues in Bovine Milk,” Bulletin of Environmental Contamination and Toxicology, Vol. 80, No. 1, 2008, pp. 5-9. doi:10.1007/s00128-007-9276-6

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