Share This Article:

Non-invasive prediction of endometriosis revisited; 3 biomarkers as Angiopoietin-2, Interleukin-1β and Vascular Endothelial Growth Factor

Abstract Full-Text HTML Download Download as PDF (Size:211KB) PP. 528-535
DOI: 10.4236/ojog.2013.37095    2,712 Downloads   4,209 Views   Citations

ABSTRACT

Introduction: Endometriosis affects up to 1 every 5 women at their reproductive age, with variable and complex symptomatology. Patients may be asymptomatic but may have pain episodes or subfertility. Its negative impact is on patients’ health and quality of life. Objective: it was to investigate the serum and peritoneal fluid (PF) concentrations of Angiopoietin- 2, Interleukin-1β, and Vascular Endothelial Growth Factor, aiming to evaluate their diagnostic performance in endometriosis. Methods: Serum and peritoneal fluid samples were taken from 112 women undergoing laparoscopy for infertility, pelvic pain or adnexal masses. 61 diagnosed with endometriosis and 51 controlled. Primary outcome was to estimate serum and PF concentrations of Angio-2, IL-1β and VEGF and secondarily correlate these concentrations to disease stages thus assuming their diagnostic potential. Results: Significant differences were found between patients and control as regards serum and PF concentration of all studied markers except serum IL-1β. Serum Angio-2 and PF VEGF showed a significantly higher level in more advanced stages of endometriosis. PF VEGF showed a positively significant correlation with the stage of the disease, spearman coefficient t = 0.442 p = 0.014. PF concentrations of Angio-2 and Serum VEGF did not show significant pattern changes with stage-related levels. Diagnostic potential of serum and PF concentrations of the 3 markers were assessed by the ROC curve. Angio-2 proved an excellent diagnostic ability for endometriosis. PF and serum VEGF proved an equal diagnostic performance, whereas, PF IL-1β was the least efficient. Based on the results, we suggested preliminary serum threshold values for these markers to be used as diagnostic or follow-up landmarks with relatively acceptable sensitivity, specificity, positive and negative predictive values. Conclusion: Non-invasive predictive biomarkers for endometriosis were Serum Angio-2, IL-1β, and VEGF independently or in combination with the estimated threshold values. Serum Angio-2 merit is considered as a novel marker for endometriosis due to its diagnostic power.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Moety, H. , Khalil, G. , Sharkawy, R. , Ghandour, M. , Moiety, F. and Salem, H. (2013) Non-invasive prediction of endometriosis revisited; 3 biomarkers as Angiopoietin-2, Interleukin-1β and Vascular Endothelial Growth Factor. Open Journal of Obstetrics and Gynecology, 3, 528-535. doi: 10.4236/ojog.2013.37095.

References

[1] Kennedy, S., Bergqvist, A., Chapron, C., D’Hooghe, T., Dunselman, G., Greb, R., Hummelshoj, L., Prentice, A., Saridogan, E. on Behalf of the ESHRE Special Interest Group for Endometriosis Endometrium Guideline Development Group (2005) ESHRE guideline for the diagnosis and treatment of endometriosis. Human Reproduction, 20, 2698-2704. doi:10.1093/humrep/dei135
[2] Vercellini, P., Trespidi, L., De Giorgi, O., Cortesi, I., Parazzini, F. and Crosignani, P.G. (1996) Endometriosis and pelvic pain: Relation to disease stage and localization. Fertility and Sterility, 65, 299-304.
[3] Leng, J.H., Lang, J.H., Dai, Y., Li, H.J. and Li, X.Y. (2007) Relationship between pain symptoms and clinicopathological features of pelvic endometriosis. Zhonghua Fu Chan, 42, 165-168.
[4] American Society for Reproductive Medicine (1997) The revised American Society of endometriosis: 1996. Fertility and Sterility, 67, 817-821. doi:10.1016/S0015-0282(97)81391-X
[5] Bedaiwy, M.A., Falcone, T., Sharma, R.K., Goldberg, J.M., Attaran, M., Nelson, D.R. and Agarwal, A. (2008) Prediction of endometriosis with serum and peritoneal fluid markers: A prospective controlled trial. Human Reproduction, 17, 426-431. doi:10.1093/humrep/17.2.426
[6] Ramey, J.W. and Archer, D.F. (1993) Fertil Steril. Peritoneal fluid: its relevance to the development of endometriosis. Fertility and Sterility, 60, 1-14.
[7] Cai, J.T., Zhang, Y.D., Zhang, Y., Yu, R., Li, M.X., Zhang, Y., Peng, G.Q. and Peng, L.X. (2008) Expression of heparanase and angiopoietin-2 in patients with endometriosis. European Journal of Obstetrics & Gynecology and Reproductive Biology, 136, 199-209. doi:10.1016/j.ejogrb.2006.09.018
[8] Wang, H., Gorpudolo, N., Li, Y., Feng, D., Wang, Z. and Zhang, Y. (2009) Elevated vascular endothelial growth factor-A in the serum and peritoneal fluid of patients with endometriosis. Journal of Huazhong University of Science and Technology—Medical Sciences, 29, 637-641. doi:10.1007/s11596-009-0520-7
[9] Scholl, B., Bersinger, N.A., Kuhn, A. and Mueller, M.D. (2009) Correlation between symptoms of pain and peritoneal fluid inflammatory cytokine concentrations in endometriosis. Gynecological Endocrinology, 25, 701-706. doi:10.3109/09513590903159680
[10] Mihalyi, A., Gevaert, O., Kyama, C.M., Simsa, P., Pochet, N., De Smet, F., De Moor, B., Meuleman, C., Billen, J., Blanckaert, N., Vodolazkaia, A., Fulop, V. and D’Hooghe, T.M. (2010) Non-invasive diagnosis of endometriosis based on a combined analysis of six plasma biomarkers. Human Reproduction, 25, 654-664. doi:10.1093/humrep/dep425
[11] Donnez, J., Smoes, P., Gillerot, S., Casanas-Roux, F. and Nisolle, M. (1998) Vascular endothelial growth factor (VEGF) in endometriosis. Human Reproduction, 13, 1686-1690. doi:10.1093/humrep/13.6.1686
[12] Lebovic, D.I., Shifren, J.L., Ryan, I.P., Mueller, M.D., Korn, A.P., Darney, P.D. and Taylor, R.N. (2000) Ovarian steroid and cytokine modulation of human endometrial angiogenesis. Human Reproduction, 15, 67-77. doi:10.1093/humrep/15.suppl_3.67
[13] Meresman, G., Buquet, R., Bilotas, M., Sueldo, C. and Baranao, R.I. (2010) Effect of vascular endothelial growth factor and interleukin-1 on apoptosis in endometrial cell cultures from patients with endometriosis and controls. Journal of Reproductive Immunology, 84, 193-198. doi:10.1016/j.jri.2009.12.002
[14] Zhang, Y., Cai, J.T., Li, M.X., Zhang, Y., Liu, H.N. and Yu, R. (2006) Expression of heparanase and angiopoietin-2 mRNA in endometriosis. Zhonghua Fu Chan Ke Za Zhi, 41, 291-294.
[15] Di Carlo, C., Bonifacio, M., Tommaselli, G.A., Bifulco, G., Guerra, G. and Nappi, C. (2009) Metalloproteinases, vascular endothelial growth factor, and angiopoietin 1 and 2 in eutopic and ectopic endometrium. Fertility and Sterility, 91, 2315-2323. doi:10.1016/j.fertnstert.2008.03.079
[16] Bourlev, V., Iljasova, N., Adamyan, L., Larsson, A. and Olovsson, M. (2010) Signs of reduced angiogenic activity after surgical removal of deeply infiltrating endometriosis. Fertility and Sterility, 94, 52-57. doi:10.1016/j.fertnstert.2009.02.019
[17] Rein, D.T., Schmidt, T., Bauerschmitz, G., Hampl, M., Beyer, I.M., Paupoo, A.V., Curiel, D.T. and Breidenbach, M. (2010) Vascular Endothelial Growth Factor (VEGF)targeted gene therapy for the treatment of endometriosis. Fertility and Sterility, 93, 2687-2694.
[18] Plank, M.J., Sleeman, B.D. and Jones, P.F. (2004) The role of the angiopoietins in tumour angiogenesis. Growth Factors, 22, 1-11. doi:10.1080/08977190310001643218
[19] Gescher, D.M., Berndorrf, U., Meyhoefer-Malik, A., Moubayed, P. and Malik, E. (2004) Immunolocalization of angiopoietin1 in human peritoneal endometriotic lesions. Fertility and Sterility, 81, 857-862. doi:10.1016/j.fertnstert.2003.09.032
[20] Kalu, E., Sumar, N., Giannopoulos, T., Patel, P., Croucher, C., Sherriff, E. and Bansal, A. (2007) Cytokine profiles in serum and peritoneal fluid from infertile women with and without endometriosis. Journal of Obstetrics and Gynaecology Research, 33, 490-495. doi:10.1111/j.1447-0756.2007.00569.x
[21] Hou, Z., Zhou, J., Ma, X., Fan, L., Liao, L. and Liu, J. (2008) Role of interleukin-1 receptor type II in the pathogenesis of endometriosis. Fertility and Sterility, 89, 42-51. doi:10.1016/j.fertnstert.2007.01.044

  
comments powered by Disqus

Copyright © 2019 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.