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Isolation of aromatic yeasts(non-Saccharomyces cerevisiae) from Korean traditional nuruks and identification of fermentation characteristics

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DOI: 10.4236/as.2013.45B025    3,222 Downloads   4,622 Views   Citations

ABSTRACT

Ethyl caproate and isoamyl alcohol are important for the quality of Yakju, one of the Korean traditional alcoholic beverages. From Korean traditional fermentation agent, Nuruk, we have isolated 8 yeasts which produced rich aromatic compounds using YEPD agar plates (1% yeast extract, 2% peptone, 2% dextrose, 2% agar) containing 50 uL cerulenin at 30℃. The isolated aromatic yeasts are identified as Pichia anomala (4 strains), Pichia fabianii (2 strains), Pichia farinose (1 strains), Geotrichum candidum (1 strains). We conducted alcohol fermentation with each of the aromatic yeasts and the compounds (ethyl caproate and isoamyl alcohol) producing range were 59.5 - 193.2 ppm and 10.8 - 91.6 ppm respectively. As a control, Fermivin®, famous aromatic wine yeast, was 89.4 ppm and 16.2 ppm respectively. We also find that the isolated Pichia anomala could produce higher level ethanol (4.2% - 5.0%, v/v) than other species (0.4% - 2.2%, v/v). Using the aromatic yeasts in fermentation industries, we expect to improve the quality of traditional alcoholic beverages.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Ho, C. J., Yeo, S. H., Park, J.-H., Choi, H. S., Gang, J. -E., Kim, S.I., Jeong, S. T. and Kim, S. R. (2013) Isolation of aromatic yeasts(non-Saccharomyces cerevisiae) from Korean traditional nuruks and identification of fermentation characteristics. Agricultural Sciences, 4, 136-140. doi: 10.4236/as.2013.45B025.

References

[1] Álvarez-Pérez, J.M., Campo, E., San-Juan, F., Coque, J.J.R., Ferreira, V. and Hernández-Orte. P. (2012) Sensory and chemical characterisation of the aroma of Prieto Picudo rosé wines: The differential role of autochthonous yeast strains on aroma profiles. Food Chemistry, 133, 284-292. doi:10.1016/j.foodchem.2012.01.024
[2] Asano, T., Inoue, T., Kurose, N., Hiraoka, N. and Kawakita, S. (1999) Improvement of isoamyl acetate productivity in Sake yeast by isolating mutants resistant to econazole. Journal of bioscience and bioengineering, 87, 697-699. doi:10.1016/S1389-1723(99)80137-6
[3] Díaz-Montaño, D.M., Délia, M.L., Estarrón-Espinosa, M. and Strehaiano, P. (2008) Fermentative capability and aroma compound production by yeast strains isolated from Agave tequilana Weber juice. Enzyme and Microbial Technology, 42, 608-616. doi:10.1016/j.enzmictec.2007.12.007
[4] Furukawa, K., Yamada, T., Mizoguchi, H. and Shodo, H. (2004) Increased alchol acetyltransferase activity by inositol limitation in Saccharomyces cerevisiae in Sake mash. Journal of bioscience and bioengineering, 96, 380-386.
[5] Hazelwood, L.A., Daran, J.M., Maris, A.J.A.van., Pronk, J. and Dickinson, J.R. (2008) The ehrlich pathway for fusel alcohol production: A century of research on Saccharomyces cerevisiae metabolism. Applied and Environmental Microbiology, 74, 2259-2266. doi:10.1128/AEM.02625-07
[6] Ichikawa, E., Hosokawa, N., HATA, Y., Abe, Y., Suginami, K. and Iimayasu, S. (1991) Breeding of a sake yeast with Improved Ethyl caproate productivity. Agricultural and Biological Chemistry, 55, 2153-2154. doi:10.1271/bbb1961.55.2153
[7] Jin, T.Y., Kim, E.S., Eun, J.B., Wang, S.J. and Wang. M.H.(2007) Changes in physicochemical and sensory characteristics of rice wine, Yakju prepared with different amount of red yeast rice. Korean Journal of Food Science and Technology, 39, 309-314.
[8] Katou, T., Namise, M. Kitagaki, H. Akao, T. and Shi- moi, H. (2009) QTL mapping of sake brewing characteristics of yeast. Journal of Bioscience and Bioengineering, 107, 383-393. doi:10.1016/j.jbiosc.2008.12.014
[9] Kim, H.R., Kim, J.H., Bai, D.H. and Ahn, B. (2012) Feasibility of brewing makgeolli using Pichia anomala Y197-13, a non-Saccharomyces cerevisiae. Journal of Microbiology and Biotechnology, 22, 1749-1757. doi:10.4014/jmb.1210.10038
[10] Liang, H.Y., Chen, J.Y., Reeves, M. and Han, B.Z. (2013) Aromatic and sensorial profiles of young Cabernet Sauvignon wines fermented by different Chinese autochthonous Saccharomyces cerevisiae strains. Food Research International: In Press, Accepted Manuscript. doi:10.1016/j.foodres.2013.01.056
[11] Miller, G.L. (1959) Use of dinitrosalicylic acid reagent for determinaion of reducing sugar. Anal. Chem. 31, 426- 428. doi:10.1021/ac60147a030
[12] NTSTS, I. (2009) Terms of liquor analysis. National Tax Service Technical Service, 41-42.
[13] NTSTS, I. (2009) Terms of liquor analysis. National Tax Service Technical Service, 38-39.
[14] Rojas, V., Gil, J.V., Piñaga, F. and Manzanares, P. (2001) Studies on acetate ester production by non-Saccharomyces wine yeasts. International Journal of Food Microbiology, 70, 283-289. doi:10.1016/S0168-1605(01)00552-9

  
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