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The Fusability of Erythrocytes as a Method for Evaluating the Instability of Their Membranes

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DOI: 10.4236/ojbiphy.2013.33023    2,207 Downloads   4,368 Views  

ABSTRACT

A novel approach to the study of erythrocytes instability based on assessment fusability of their plasma membrane is presented. The fusability of erythrocytes normal blood, stored for 7 days at 4°C, and type 2 patients with diabetes were studied. Human erythrocytes were fused by incubation with La3+ at final concentrations from 50 to 200 μM. Erythrocytes aggregates were incubated at 37°C for 120 min. The fusability of erythrocytes was evaluated using a light microscope. It was shown that La3+ induced extensive fusion of erythrocytes after 7 days of blood storage and erythrocytes of patients with diabetes. Incubation of normal erythrocytes at 37°C for 120 min does not induce cell fusion.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Y. Sheremet’ev, A. Popovicheva, M. Rogozin and G. Levin, "The Fusability of Erythrocytes as a Method for Evaluating the Instability of Their Membranes," Open Journal of Biophysics, Vol. 3 No. 3, 2013, pp. 198-200. doi: 10.4236/ojbiphy.2013.33023.

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