Share This Article:

Relation between Amino Acids Profiles and Recalcitrancy of Cell Growth or Salt Tolerance in Tissue and Protoplast Cultures of Three Mangrove Species, Avicennia alba, Bruguiera sexangula, and Sonneratia alba

Abstract Full-Text HTML Download Download as PDF (Size:340KB) PP. 1366-1374
DOI: 10.4236/ajps.2013.47167    3,367 Downloads   4,867 Views   Citations


Amino acids profiles were investigated in tissues, cultured cells, i.e. callus or suspension cells, and their protoplasts of three mangrove species, Avicennia alba, Bruguiera sexangula, and Sonneratia alba. Original tissues of cultured cells of three mangrove species were cotyledons and hypocotyls, leaves, and cotyledons, respectively. In protoplasts isolated from cultured cells, glutamine and alanine were the major amino acids. Different contents of glycine, proline and serine were observed among protoplasts of three mangrove species. Large differences in the major amino acids were found among cultured cells and their protoplasts while no difference was found between callus and suspension cells independent of additional salt in culture medium. Protoplasts of original tissues, young leaves and cotyledons, contained alanine and glutamine and/or asparagine. In suspension cells of B. sexangula, total contents of amino acids were low while their protoplasts showed similar value as of other samples. Protoplasts of leaf and cotyledons of A. alba and cotyledons of A. lanata, A. marina and S. alba were also investigated. The total contents of amino acids and their profiles might be related to the recalcitrance for the growth and salt tolerance or halophilic nature of cells and basal media used for the maintenance of cell cultures or protoplast cultures of the mangrove species. This is the first report on callus induction from hypocotyls of A. alba.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

S. Tsuchiya, S. Ogita, Y. Kawana, T. Oyanagi, A. Hasegawa and H. Sasamoto, "Relation between Amino Acids Profiles and Recalcitrancy of Cell Growth or Salt Tolerance in Tissue and Protoplast Cultures of Three Mangrove Species, Avicennia alba, Bruguiera sexangula, and Sonneratia alba," American Journal of Plant Sciences, Vol. 4 No. 7, 2013, pp. 1366-1374. doi: 10.4236/ajps.2013.47167.


[1] P. B. Tomlinson, “The Botany of Mangroves,” Cambridge University Press, New York, 1986.
[2] M. Spalding, M. Kainuma and L. Collins, “World Atlas of Mangroves,” Gutenberg Press, Malta, 2010.
[3] S. Ogita, E. C. Yeung and H. Sasamoto, “Histological Analysis in Shoot Organogenesis from Hypocotyl Explants of Kandelia candel (L.) Druce,” Journal of Plant Research, Vol. 117, No. 6, 2004, pp. 457-464. doi:10.1007/s10265-004-0180-4
[4] M. Akatsu, Y. Hosoi, H. Sasamoto and H. Ashihara, “Purine Metabolism in Cells of a Mangrove Plant, Sonneratia alba, in Tissue Culture,” Journal of Plant Physiology, Vol. 149, No. 1-2, 1996, pp. 133137. doi:10.1016/S0176-1617(96)80185-4
[5] Y. Kawana, R. Yamamoto, Y. Mochida, K. Suzuki, S. Baba and H. Sasamoto, “Generation and Maintenance of Suspension Cultures from Cotyledons and Their Organogenic Potential of Two Mangrove Species, Sonneratia alba and S. caseolaris,” Plant Biotechnology Reports, Vol. 1, No. 4, 2007, pp. 219-226. doi:10.1007/s11816-007-0035-2
[6] R. Yamamoto, Y. Kawana, R. Minagawa and H. Sasamoto, “Effects of Carbon and Nitrogen Sources on Induction of Cell Proliferation in Tissue Cultures of a Mangrove Plant, Sonneratia caseolaris,” Mangrove Science, Vol. 6, 2009, pp. 1-8.
[7] T. Murashige and F. Skoog, “A Revised Medium for Rapid Growth and Bioassay with Tobacco Tissue Cultures,” Physiologia Plantarum, Vol. 15, 1962, pp. 473497. doi:10.1111/j.1399-3054.1962.tb08052.x
[8] M. Kura-Hotta, M. Mimura, T. Tsujimura, S. Washitani-Nemoto and T. Mimura, “High Salt-Treatment-Induced Na+ Extrusion and Low Salt-Treatment-Induced Na+ Accumulation in Suspension-Cultured Cells of the Mangrove Plant Bruguiera sexangula,” Plant Cell Environment, Vol. 24, No. 10, 2001, pp. 1105-1112. doi:10.1046/j.0016-8025.2001.00761.x
[9] T. Mimura, M. Mimura, S. Washitani-Nemoto, K. Sakano, T. Shimmen and S. Siripatanadilok, “Efficient Callus Initiation from Leaf of Mangrove Plant, Bruguiera sexangula in Amino Acid Medium: Effect of NaCl on Callus Initiation,” Journal of Plant Research, Vol. 110, No. 1, 1997, pp. 25-29. doi:10.1007/BF02506839
[10] S. Hayashi, S. Kuriyama, Y. Kawana, A. Hasegawa, A. Kurita, R. Minagawa and H. Sasamoto, “Stimulatory Effects of Sea Salts on Cell Growth in Liquid Culture of Avicenniaceae Mangrove,” Plant Biotechnology, Vol. 26, No. 5, 2009, pp. 561-564. doi:10.5511/plantbiotechnology.26.561
[11] Y. Kawana and H. Sasamoto, “Stimulation Effects of Salts on Growth in Suspension Culture of a Mangrove Plant, Sonneratia alba, Compared with Another Mangrove, Bruguiera sexangula and Non-Mangrove Tobacco BY-2 Cells,” Plant Biotechnology, Vol. 25, No. 2, 2008, pp. 151-155. doi:10.5511/plantbiotechnology.25.151
[12] T. Fukumoto, T. Nakamura, M. Suzuki, S. Ogita, T. Mimura and H. Sasamoto, “Different Effects of Four Salts and pHs on Protoplast Cultures of a Mangrove, Bruguiera sexangula Suspension Cells, Populus alba Leaves and Tobacco BY-2 Cells,” Plant Biotechnology, Vol. 21, No. 3, 2004, pp. 177-182. doi:10.5511/plantbiotechnology.21.177
[13] A. Hasegawa, S. Hayashi, A. Kurita, F. Kaai, Y. Kawana, T. Fukumoto and H. Sasamoto, “Stimulatory and Inhibitory Effects of Abscisic Acid on Cell Growth in Protoplast Cultures and the Relation to Its Endogenous Levels in Avicenniaceae Mangrove Cells,” Mangrove Science, Vol. 8, 2011, pp. 11-18. doi:10.1007/s11816-012-0251-2
[14] A. Hasegawa, A. Kurita, S. Hayashi, T. Fukumoto and H. Sasamoto, “Halophilic and Salts Tolerant Protoplast Cultures of Mangrove Plants, Sonneratia alba and Avicennia alba,” Plant Biotechnology Reports, Vol. 7, No. 2, 2013, pp. 205-209. doi:10.1007/s11816-012-0251-2
[15] S. Ogita, H. Sasamoto, E. C. Yeung and T. A. Thorpe, “The Effects of Glutamine on the Maintenance of Embryogenic Cultures of Cryptomeria japonica,” In Vitro Cellular and Developmental Biology Plant, Vol. 37, No. 2, 2001, pp. 268-273. doi:10.1007/s11627-001-0048-4
[16] H. Sasamoto, Y. Wakita and S. Baba, “Effect of High Sorbitol Concentration on Protoplast Isolation from Cotyledons of Mangroves, Avicennia marina, and A. lanata,” Plant Biotechnology, Vo. 14, No. 2, 1997, pp. 101-104. doi:10.5511/plantbiotechnology.14.101
[17] Y. Kawana, H. Sasamoto, Y. Mochida and K. Suzuki, “Leaf Protoplast Isolation from Eight Mangrove Species of Three Different Families; Avicenniaceae, Rhizophoraceae and Sonneratiaceae,” Mangrove Science, Vol. 3, 2004, pp. 25-31.
[18] Y. Kawana, F. Kaai and H. Sasamoto, “Abscisic Acid Stimulates Cell Divisions in Cultures of Protoplasts Isolated from Cotyledons and Suspension Cells of a Mangrove Plant Sonneratia alba: Small-Scale Measurements of Abscisic Acid and Gibberellins in Protoplasts,” Mangrove Science, Vol. 6, 2009, pp. 9-15.
[19] H. Kotaniguchi and M. Kawakatsu, “Automatic Amino Acid Analysis Utilizing 4-Fluoro-7-nitobenzo-s-oxa-diazole,” Journal of Chromatography, Vol. 420, 1987, pp. 141-145.
[20] H. Ashihara, K. Adachi, M. Otawa, E. Yasumoto, Y. Fukushima, M. Kato, H. Sano, H. Sasamoto and S. Baba, “Compatible Solutes and Inorganic Ions in the Mangrove Plant, Avicennia marina and Their Effects on the Activities of Enzymes,” Zeitshrift fur Naturforschung, Vol. 52c, 1997, pp. 433-440.
[21] A. Hasegawa, S. Hayashi, A. Kurita, Y. Kawana, T. Fukumoto and H. Sasamoto, “Isolation and Cultures of Suspension Cells of Sonneratia alba: Development of Bioassay Method of Allelopathy at Cellular Levels,” Abstracts of 14th MTG of Japanese Mangrove Society, 2008, p. 14.
[22] A. Kurita, S. Kuriyama, Y. Kawana and H. Sasamoto, “Characteristics of Basal Medium for Suspension Cultures and Their Protoplasts Cultures of Avicennia alba and Bruguiera sexangula,” Abstracts of 13th mtg of Jpn Mangrove Society, 2007, p. 6.
[23] S. Tsuchiya, R. Minagawa, A. Inoue and H. Sasamoto, “Development of Cell Culture System from Hypocotyls of Avicennia alba: Callus Proliferation and Their Protoplasts Isolation and Cultures,” Abstracts of 16th MTG of Japanese Mangrove Society, 2010, p. 15.
[24] C. N. Chowdhry, A. K. Tyagi, N. M. Maheshwari and S. C. Maheshwari, “Effect of l-Proline and l-Tryptophan on Somatic-Embryogenesis and Regeneration of Rice (Oryza sativa L. cv. Pusa169,” Plant Cell Tissue and Organ Culture, Vol. 32, No. 3, 1993, pp. 357-361. doi:10.1007/BF00042300
[25] S. Ogita, H. Sasamoto, H. Ashihara and S. Baba, “Levels of Glycinebetaine and Amino Acids in Cotyledons Protoplasts of a Mangrove, Avicennia marina,” Proceedings of 111th MTG of Japanese Forest Society, 2000, p. 604.
[26] R. Waditee, T. Hibino, Y. Tanaka, T. Nakamura, A. Incharoensakdi, S. Hayakawa, S. Suzuki, Y. Futsuhara, Y. Kawamitsu, T. Takabe and T. Takabe, “Functional Characterization of Betaine/Proline Transporters in Betaine Accumulating Mangrove,” Journal of Biological Chemistry, Vol. 277, 2002, pp. 18373-18382. doi:10.1074/jbc.M112012200
[27] H. Sasamoto and S. Ogita, “Endogenous Plant Hormones in Protoplasts of Embryogenic Cells of Conifers,” In: N. Morohoshi and A. Komamine, Eds., Molecular Breeding of Woody Plants, Elsevier Science, Amsterdam, 2001, pp. 279-288.

comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.