Research of Quality Standards for Stachydrine Hydrochloride in Chinese Medicine TJF Granule

Yao Lu, Wei Yu, Pingniang Shen, T. J. Khoo, C. Wiart

Faculty of Sciences, School of Biomedical Sciences, University of Nottingham (Malaysia Campus), Semenyih, Malaysia.
National Engineering Research Center for Traditional Chinese Medicine, Shanghai, China..

DOI: 10.4236/pp.2013.43040                

Abstract

A high-performance liquid chromatographic method was applied to the determination of stachydrine hydrochloride concentration in TJF granule (Chinese name: Tiao-Jing-Fang), using a mobile phase of methanol-acetonitrile (50:50, v/v) by the Agilent Kromasi NH2 column (250 mm × 4.6 mm, 5 μm, S/N: 22N25110). Detection wavelength was 201 nm. The result revealed good linearity of stachydrine hydrochloride and was obtained within the range of 0.20 - 1.98 μg/mL (R = 0.9995). The average recovery was 97.01%; the relative standard deviation (RSD) was 0.19%. To the best of our knowledge, this is the first report dedicated to the determination of stachydrine hydrochloride by the evaporative light scattering detector-high-performance liquid chromatographic (ELSD-HPLC) method.

Keywords

TJF Granule; Quality Standards; Traditional Chinese Medicine; Evaporative Light Scattering Detector (ELSD); High-Performance Liquid Chromatographic (HPLC) Method; Stachydrine Hydrochloride; Pharmaceutical Analysis; Determination

Conflicts of Interest

The authors declare no conflicts of interest.

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