Preliminary Report of PGR’s Influence to Multiple Shoots Induction and Plant Regeneration on Plumbago auriculata

Yi Chen, Suping Gao

Institute of Landscape Architecture, Sichuan Agriculture University, Chengdu, China..

DOI: 10.4236/ajps.2013.45A004                

Abstract

With the explants of young nodals and leaves of Plumbago auriculata, we studied multiple shoots induction and plant regeneration in the medium with different PGR proportions. The results showed the nodals of Plumbago auriculata could be used as the explant to induce multiple shoots. After culturing young nodals for 30 d, optimum single shoot formation was achieved on MS medium supplemented with 0.3 mg/L 6-BA and the induction rate was 70.79%. After being transferred to the medium containing 1.0 mg/L 6-BA, 1.0 mg/L NAA and 0.2 mg/L IAA for 1 - 2 generation, single shoot was induced to form multiple shoots with the multiplication rate was 506.45%. After multiple shoots grew to 3 cm high, the multiple shoots were cut into single shoots and transferred onto half-strength MS medium supplemented with 0.5 mg/L NAA and the rooted rate was 94.33%. Finally the intact plants were obtained.

Keywords

Plumbago auriculata; Multiple Shoots; Induction; Plant Regeneration; PGR

Conflicts of Interest

The authors declare no conflicts of interest.

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