Isolation and expression analysis of CBF4 from Vitis amurensis associated with stress

Chang Dong, Meng Zhang, Zhiying Yu, Junpeng Ren, Yang Qin, Bailin Wang, Lizhen Xiao, Zhen Zhang, Jianmin Tao

College of Horticulture, Nanjing Agricultural University, Nanjing, China.
Department of Horticulture, Heilongjiang Academy of Agricultural Science, Harbin, China.

DOI: 10.4236/as.2013.45032                

Abstract

The C-repeat Binding Factor/Dehydration Responsive Element Binding factor (CBF/DREB) responsive pathway is involved in plant response to abiotic stress. In this study, we report the isolation of VaCBF4 (a complete cDNA) and its promoter from Vitis amurensis through rapid amplification of cDNA ends and genome walking techniques, respectively. The CBF4 transcript accumulation of V. amurensis increased under cold, salinity, and abscisic acid (ABA) and salicylic acid (SA) treatments, whereas that of Vitis vinifera showed a different change. The transcript levels of VaCBF4 inthe roots, stems, leaves, and petioles under cold, salinity, and ABA and SA treatments were up-regulated in a timedependent manner. The presence of the cis-elements MBC, ABRE, and as-2-box in the VaCBF4 promoter further confirmed that this promoter is a component of the CBF transduction pathway, which is involved in plant response to multistress.

Keywords

Vitis amurensis; CBF4; Stress; Transcript Accumulation

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Willimsky, G., et al. (1992) Characterization of cspB, a Bacillus subtilis inducible cold shock gene affecting cell viability at low temperatures. Journal of Bacteriology, 174, 6326-6335.
[2]	Liu, J., et al. (2002) Cold signalling associated with vernalization in Arabidopsis thaliana does not involve CBF1 or abscisic acid. Physiologia Plantarum, 114, 125-134. doi:10.1034/j.1399-3054.2002.1140117.x
[3]	Yamaguchi-Shinozaki, K. and Shinozaki, K. (1994) A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. The Plant Cell Online, 6, 251-264. doi:10.2307/3869643
[4]	Baker, S.S., Wilhelm, K.S. and Thomashow, M.F. (1994) The 5’-region of Arabidopsis thaliana cor15a has cis-acting elements that confer cold-, droughtand ABA-regulated gene expression. Plant Molecular Biology, 24, 701-713. doi:10.1007/BF00029852
[5]	Jiang, C., Iu, B. and Singh, J. (1996) Requirement of a CCGAC cis-acting element for cold induction of the BN115 gene from winter Brassica napus. Plant Molecular Biology, 30, 679-684. doi:10.1007/BF00049344
[6]	Chinnusamy, V., Zhu, J.K. and Sunkar, R. (2010) Gene regulation during cold stress acclimation in plants. Methods in Molecular Biology, 639, 39-55. doi:10.1007/978-1-60761-702-0_3
[7]	Medina, J.N., et al. (1999) The Arabidopsis CBF gene family is composed of three ggenes encoding AP2 domain-containing proteins whose expression is regulated by low temperature but not by abscisic acid or dehydration. Plant Physiology, 119, 463-470. doi:10.1104/pp.119.2.463
[8]	Haake, V., et al. (2002) Transcription factor CBF4 is a regulator of drought adaptation in Arabidopsis. Plant Physiology, 130, 639-648. doi:10.1104/pp.006478
[9]	Ito, Y., et al. (2006) Functional analysis of rice DREB1/ CBF-type transcription factors involved in cold-responsive gene expression in transgenic rice. Plant & Cell Physiology, 47, 141-153. doi:10.1093/pcp/pci230
[10]	Yang, W., et al. (2011) Dwarf apple MbDREB1 enhances plant tolerance to low temperature, drought, and salt stress via both ABA-dependent and ABA-independent pathways. Planta, 233, 219-229. doi:10.1007/s00425-010-1279-6
[11]	Polashock, J.J. (2010) Functional identification of a C-repeat binding factor transcriptional activator from blueberry associated with cold acclimation and freezing tolerance. Journal of the American Society for Hortcultural Science, 135.
[12]	Kitashiba, H., et al. (2004) Expression of a sweet cherry DREB1/CBF ortholog in Arabidopsis confers salt and freezing tolerance. Journal of Plant Physiology, 161, 1171-1176. doi:10.1016/j.jplph.2004.04.008
[13]	El Kayal, W. (2006) Expression profile of CBF-like transcriptional factor genes from Eucalyptus in response to cold. Journal of Experimental Botany, 57, 2455-2469. doi:10.1093/jxb/erl019
[14]	Kong, Q.S. (2004) Grape in China. Chinese Agricultural Science and Technique, Beijing.
[15]	Xiao, H., et al. (2008) CBF4 is a unique member of the CBF transcription factor family of Vitis vinifera and Vitis riparia. Plant, Cell & Environment, 31, 1-10.