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Determination of Tramadol in Human Serum by Capillary Electrophoresis with the End-Column Electrochemiluminescence Detection

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DOI: 10.4236/ojbiphy.2013.32015    2,823 Downloads   6,349 Views  

ABSTRACT

A capillary electrophoresis (CE) coupled with end-column electrochemiluminescence (ECL) detection method for the analysis of tramadol (TMD) has been investigated. ECL detection was working electrode biased at 1.2 V in a 20mmol·L-1 sodium phosphate buffer (pH = 8.0) containing 5 mmol·L-1 Ru (where bpy = 2,2-bipyridyl). Linear correlation (r 0.997) between ECL intensity and drug concentration was obtained in the range 3 × 10-4 - 6 × 10-6 mol·L-1. The limits of detection (LODs) for tramadol in water was 3.012 × 10-8 mol·L-1(S/N = 3). The relative standard deviation values on peak size (10-5 mol·L-1 level) and migration time for the tramadol were 4.58% and 1.39% (n = 10), respectively. Applicability of the CE-ECL method to the analysis of human serum spiked with tramadol was examined

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

C. Zhang, T. Liu, X. Zhang and E. Liu, "Determination of Tramadol in Human Serum by Capillary Electrophoresis with the End-Column Electrochemiluminescence Detection," Open Journal of Biophysics, Vol. 3 No. 2, 2013, pp. 121-127. doi: 10.4236/ojbiphy.2013.32015.

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