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Response of Explants of Calli Rice (Oryza sativa L.) Japonica cv. “Ilmi” to Gene Transformation Using Agrobacterium tumefaciens-Mediated

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DOI: 10.4236/ajps.2013.44102    3,413 Downloads   5,496 Views   Citations

ABSTRACT

The AtBI-1 gene encoding the Arabidopsis thaliana Bax inhibitor was introduced into Japonica cultivars of rice (Ilmi) by Agrobacterium-mediated transformation, and a large number of transgenic plants were produced. The neomycin phosphotransferase II (NPTII) gene was used as a selectable marker. The activity of neomycin phosphotransferase could be successfully detected in transgenic rice calluses. Introduction of the AtBI-1 gene was also confirmed by PCR using AtBI-1 specific oligonucleotide primers in regenerated plants. Stable integration and expression of the AtbI-1 gene in plants were confirmed by GFP analysis.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

F. Safitri, M. Ubaidillah, M.   and K. Kim, "Response of Explants of Calli Rice (Oryza sativa L.) Japonica cv. “Ilmi” to Gene Transformation Using Agrobacterium tumefaciens-Mediated," American Journal of Plant Sciences, Vol. 4 No. 4, 2013, pp. 838-843. doi: 10.4236/ajps.2013.44102.

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