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Immunoresponse to Allogeneic Synovial or Xenogenic Mesenchymal Stromal Cells in a Co-Culture Model

DOI: 10.4236/ojcb.2012.21001    3,565 Downloads   7,352 Views   Citations

ABSTRACT

The purpose of our investigations was to measure, in a co-culture condition, the immunoresponse to allogeneic or xenogenic cells, selected as potential sources for cell therapy of arthritis. We challenged human spleen-derived cells (hSpl) by three different mechanisms: 1) exposure to donor allogeneic or xenogeneic cellular antigens; 2) exposure to donor cells transduced with adenoviral antigens (Ad) and 3) lipopolysaccharide (LPS), a known inflammatory immunostimulant. The immunoresponse to allogeneic human synovial-derived mesenchymal stromal cells alone or transduced with adenoviral green fluorescent protein (hSD-MSC or hSD-MSC/GFP) or the immunoresponse to xenogeneic equine mesenchymal stromal cells (eqMSC) or equine dermal fibroblasts (eqDFb), characterized by the proportion of CD3+, CD4+, and CD8+ human splenocytes (hSpl), was measured on Day 0 and Day 6 of co-culture by flow cytometry. In culture with hSD-MSC, hSD-MSC/GFP, eqDFb, or eqMSC, the proportion of CD3+ and CD8+ hSpl increased with time in culture but not with exposure to cell alloor xeno-antigens. Both hSD-MSC and hSD-MSC/GFP increased in number during culture and were not affected in viability or proliferation by co-culture with allogeneic hSpl. In this in vitro, primary exposure study, hSpl demonstrated a natural selection and adaptation to a short-term cell culture environment, and that neither allogeneic nor xenogeneic cell antigens incited a greater cellular immunoactivation than co-cultured hSpl alone.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

S. Jump, D. Smith, D. Flanigan and A. Bertone, "Immunoresponse to Allogeneic Synovial or Xenogenic Mesenchymal Stromal Cells in a Co-Culture Model," CellBio, Vol. 2 No. 1, 2012, pp. 1-9. doi: 10.4236/ojcb.2012.21001.

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