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Functional Analysis of a Wilt Fungus Inducible PR10-1 Gene from Cotton

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DOI: 10.4236/ajps.2013.42A054    4,233 Downloads   6,705 Views   Citations

ABSTRACT

Early stage expression of PR10 combined with phytoalexins contributed to Verticillium wilt resistance in cotton. In order to analysis the activities of PR10 proteins during pathogens’ infection, we cloned a Verticillium-induced PR10 (GbPR10-1) gene from cotton (Gossypium barbadense) and compared its expression patterns and domains with other PR10 proteins. Bioinformatics indicated that GbPR10-1 showed the lowest similarity with other 12 different PR10 genes in cotton (Upland and sea-island cotton). Expression profiles showed that GbPR10-1 gene instantly up-regulated after infection by V. dahliae in the sea-island cotton plants. GbPR10-1 was also induced by environmental stimulus including heat, submergence and salt, and ethylene but not by ABA and salicylic acid. The GbPR10-1 protein expressed in E. coli BL21 demonstrated that it had a low ribonuclease-like activity in vitro, and could inhibit V. dahliae hyphae growth but not its spores. Comparison analysis of GbPR10-1 (from resistant species) and GhPR10-1 (from susceptible species) responding to V. dahliae infection, only GbPR10-1 gene was strongly induced in the sea-island cotton plants (incompatible response), indicating that PR10-1 genes was linked to resistance signal. In summary, the earlier activation of GbPR10-1 gene, as the index of resistance response, would be aid to block the

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

L. Chen, N. Sun, J. Wang, H. Ling, L. Zhang and K. Zuo, "Functional Analysis of a Wilt Fungus Inducible PR10-1 Gene from Cotton," American Journal of Plant Sciences, Vol. 4 No. 2A, 2013, pp. 417-426. doi: 10.4236/ajps.2013.42A054.

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