Share This Article:

Chemo-Divergence in Essential Oil Composition among Thirty One Core Collections of Ocimum sanctum L. Grown under Sub-Tropical Region of Jammu, India

Abstract Full-Text HTML XML Download Download as PDF (Size:766KB) PP. 302-308
DOI: 10.4236/ajps.2013.42040    2,762 Downloads   4,361 Views   Citations

ABSTRACT

Evaluation of thirty one core collections of Ocimum sanctum L. synonyms O. tenuiflorum L. collected from different ecological regions representing contrasting environment of India was carried out. All the collections were grown under sub-tropical region of Jammu, India. Study revealed wide range of variability in quantitive and qualitative attributes of oil. Essential oil content ranged between 0.16% ± 0.01% - 0.55% ± 0.08% showing the presence of fifteen constituents. Methyl eugenol (1.54% - 93.16%) and Eugenol (0.06% - 70.41%), were the major constituent. The other major constituent of the oil was β-Caryophyllene (4.60% - 33.77%) which was detected in almost all the collections. Borneol, Copane, α Caryophyllene were other constituents detected in almost all the accessions. α selinene was detected in traces in only three accessions (OS-01, OS-03, OS-50) and β-selinene was detected in four accessions (OS-01, OS-03, OS-50, OS-72. Accession OS-70 collected from Patna, showed distinct chemical profile having β-Elemene (32.81%), β-Cary- ophyllene (16.37%), Germacrene-D (18.05%), β-Ocimene (17.69%) and Copane (5.738%). Being distinct in oil profiling, Patna collection was designated as distinct chemotype. Collections OS-50 from Gwalior from Central India and OS-59 from Rajkot Western India have been identified as methyl eugenol (93.16%) and eugenol (70.41%) rich geno- types. The data collected provided useful information with respect to composition of essential oil among core collection evaluated representing various agro-climatic zones.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

S. Kitchlu, R. Bhadauria, G. Ram, K. Bindu, R. Khajuria and A. Ahuja, "Chemo-Divergence in Essential Oil Composition among Thirty One Core Collections of Ocimum sanctum L. Grown under Sub-Tropical Region of Jammu, India," American Journal of Plant Sciences, Vol. 4 No. 2, 2013, pp. 302-308. doi: 10.4236/ajps.2013.42040.

References

[1] S. S. Balyan and P. Pushpangadan, “A Study on the Taxonomic Status and Geographic Distribution of the Genus, Ocimum,” PAFAI, Vol. 10, No. 2, 1988, pp. 13-19.
[2] S. Dutt, “Essential Oil of Ocimum sanctum L.,” Proceedings of Indian Academy of Sciences, Section A, Vol. 9, No. 1, 1939, pp. 72-77.
[3] V. Singh, S. Amdekar and O. Verma, “Ocimum sanctum (tulsi): Bio-Pharmacological Activities,” Webmed Central Pharmacology, Vol. 1, No. 10, 2010, pp. 1-7.
[4] R. N.Chopra, D. N. Roy, S. M. Ghosh and J. Malaria, “The Insecticidal and Larvicidal Action of the Essential Oils of Ocimum basilicum [L.] and Ocimum sanctum [L.],” Inxt. India, Vol. 4, 1941, pp. 109-112.
[5] R. Dupcar, “Gonopoti Chemical Composition of Ocimum sanctum,” Congreso Luso-Espanol Farm, Vol. 3, 1952, pp. 187-191.
[6] J. E. Simon and D. R. Bubenheim, “Field Performance of American Basil Varieties,” Journal of Herb, Spice and Medicinal Plant, Vol. 6, No. 1, 1987, pp. 1-4.
[7] J. E. Simon, J. Quinn and R. G. Murray, “Basil: A Source of Essential Oils,” Advances in New Crops, Timber Press, Portland, 1990, pp. 484-489.
[8] S. Laskar, S. Majumdar, S. Ghosh, S. Laskar, S. Majumdar and S. Ghosh, “Variation of Major Constituents of Essential Oil of the Leaves of Ocimum sanctum L.,” Journal of the Indian Chemical Society, Vol. 65, No. 4, 1988, pp. 301-302.
[9] M. L. Maheshwari, B. M. Singh and R. Gupta, “Essential Oil of Sacred Basil (Ocimum sanctum),” Indian Perfumer, Vol. 31, No. 2, 1987, pp. 137-145.
[10] H. Skaltsa-Diamantidis, O. Tzakou, A. Loukis and N. Argyriadou, “Analysis of the Essential Oil of Ocimum sanctum L.,” Plantes Medicinales et Phytotherapie, Vol. 24, No. 2, 1990, pp. 79-81.
[11] R. F. Vieira, R. J. Grayer, A. Paton and J. E. Simon, “Genetic Diversity of Ocimum gratissimum L. Based on Volatile Oil Constituents, Flavonoids and RAPD Markers,” Biochemical Systematics and Ecology, Vol. 29, No. 3, 2001, pp. 287-304. doi:10.1016/S0305-1978(00)00062-4
[12] S. D. Ahmad and I. Khaliq, “Top of Form Morpho-Molecular Variability and Heritability in Ocimum sanctum Genotypes from Northern Himalayan Region of Pakistan,” Pakistan Journal of Biological Sciences, Vol. 5, No. 10, 2002, pp. 1084-1087. doi:10.3923/pjbs.2002.1084.1087
[13] R. P. Adams, “Identifi-cation of Essential Oil Components by Gas Chromatogra-phy/Mass Spectroscopy,” Allured Publishing Corporation, Carol Stream, 2006.
[14] M. Anwar, D. D. Patra, S. Hand, A. Kumar, A. A. Naqvi and S. P. S. Khanuja, “Effect of Organic Manures and Inorganic Fertilizer on Growth, Herb and Oil Yield, Nutrient Accumulation, and Oil Quality of French Basil,” Soil Science and Plant Analysis, Vol. 36, No. 13-14, 2005, pp. 1737-1746. doi:10.1081/CSS-200062434
[15] K. Bowes and V. D. Zheljazkov, “Ocimum sanctum L. and Ocimum basilicum L. Grown in Nova Scotia, Canada Show Potential as Essential Oil Crops,” Journal of the American Society for Horticultural Science, Vol. 129, 2004, pp. 789-794.
[16] M. Marotti and R. Piccaglia and E. Giovanelli, “Differences in Essential Oil Composition of Basil (Ocimum basilicum L.) Italian Cultivars Related to Morphological Characteristics,” Journal of Agricultural and Food Chemistry, Vol. 44, No. 12, 1996, pp. 3926-3929. doi:10.1021/jf9601067
[17] V. D. Topalov, “Essential Oil and Medicinal Plants,” Hr. G. Danov Press, Plovdiv, 1962, pp. 106-153.
[18] J. A. Pino, E. Roncal, A. Rosado and I. Goire, “The Essential Oil of Ocimum basilicum L. from Cuba,” Journal of Essential Oil Research, Vol. 6, No. 1, 1994, pp. 89-90. doi:10.1080/10412905.1994.9698333
[19] S. B. Wetzeil, H. Kruger, K. Hammer and K. Bachmann, “Investigations on Morphological and Molecular Variability of Ocimum L. Spe-cies,” Journal of Herb, Spice and Medicinal Plant, Vol. 9, 2002, pp. 8183-8188.
[20] J. E. Simon, M. R. Morales, W. B. Phippen, R. F. Vieira and Z. Hao, In: J. Janick and J. E. Simon, Eds., Perspectives on New Crops and New Uses, Basil: A Source of Aroma Compounds and Popular Culinary and Ornamental Herb, 1999, pp. 499-505.
[21] R. D. Gang, N. L. C. Zubieta, F. Chen, T. Beuerle, E. Lewinsohn, P. J. Noel and E. Pichersky, “Characterization of Phenylpropene O-Methyltransferases from Sweet Basil. Facile Change of Substrate Specificity and Con-vergent Evolution within a Plant O-Methyltransferase Family,” The Plant Cell, Vol. 14. No. 2, 2002, pp. 505-519. doi:10.1105/tpc.010327
[22] J. Grossman, “Botanical pesticides in Africa,” Integrated Pest Management Programme Practice, Vol. 15, 1993, pp. 1-9.
[23] C. Sisk, H. Shorey, R. Gerber and L. Gaston, “Semiochemicals That Disrupt Foraging by the Argentine Ant (Hymenoptera: Formicidae): Laboratory Bioassays,” Journal of Economic Entomology, Vol. 89, 1985, pp. 381-385.
[24] D. Obeng-Ofori and C. Reichmuth, “Bioactivity of Eugenol, a Major Component of Essential Oil of Ocimum suave (Wild.) against Four Species of Stored-Product Coleoptera,” Integrated Pest Management Programme Practice, Vol. 43, 1997, pp. 89-94.
[25] N. Chatterjee, Sukul, S. Laskar and S. Ghoshmajumdar, “Nematicidal Principles from Two Species of Lamiaceae, Ocimum sanctum and Ocimum basilicum,” The Journal of Nematology, Vol. 14, 1982, pp. 118-122.
[26] N. Sangwan, B. Verman, K. Verma and K. Dhindsa, “Nematicidal Activity of Some Essential Plant Oils,” Pesticide Science, Vol. 28, No. 3, 1990, pp. 331-335. doi:10.1002/ps.2780280311
[27] M. Karapinar and S. Aktug, “Inhibition of Foodborne Pathogens by Thymol, Eugenol, Menthol and Anethole,” International Journal of Food Micro-biology, Vol. 4, 1987, pp. 161-166.
[28] S. Adams and M. Weidenborner, “Mycelial Deformations of Cladosporium her-barum Due to the Application of Eugenol or Carvacrol,” Journal of Essential Oil Research, Vol. 8, No. 5, 1996, pp. 535-540. doi:10.1080/10412905.1996.9700682
[29] S. Miyao, “Inhibitory Effects of Ethanol Extract of Mace and Eugenol on the Growth of Microorganisms Isolated from Vienna Sausages,” Nihon Shokuhin Eisei Gakkai, Vol. 16, No. 6, 1975, pp. 412-416. doi:10.3358/shokueishi.16.412
[30] R. Shukla and V. Prasad, “Population Fluctuations of the Oriental Fruit Fly, Dacus Dorsalis Hendel, in Relation to Hosts and Abiotic Factors,” Tropical Pest Management, Vol. 31, No. 4, 1985, pp. 273-275. doi:10.1080/09670878509370999
[31] H. Kruger, S.B. Wetzel and B. Zeiger, “The Chemical Variability of Ocimum Species,” Journal of Herb, Spice and Medicinal Plant, Vol. 9, No. 4, 2002, pp. 335-344. doi:10.1300/J044v09n04_11
[32] T. I. Knishevetskaya, “Composition of Essential Oil of Hybrid Basil,” Trudy Gonsu-darrst, Nikitakoyo Botan, Soda, Vol. 27, 1939, pp. 29-36.
[33] J. A. Javanmardi, A. Khalighi, H. P. Kashi, Bais and J.M. Vivanco, “Chemical Characterization of Basil (Ocimum basilicum L.) Found in ‘Local’ Accessions and Used in Traditional Medicines in Iran,” Journal of Agricultural and Food Chemistry, Vol. 50, 2002, pp. 5878-5883. doi:10.1021/jf020487q

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.