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Air plasma for medical applications

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DOI: 10.4236/jbise.2012.59061    4,448 Downloads   7,234 Views   Citations
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The design and the electric and emission characteristics of two handheld air plasma spray generators are presented. The plasma is generated by 60 Hz periodic discharges between two concentrically cylindrical electrodes. A ring magnet is used to rotate arc discharges, which sprays outward by an air flow. The rotation of arc discharges keeps the generated plasma in non-equilibrium state and at relatively low temperature (<55°C). The plasma effluent yet contains high energy electrons which dissociate molecular oxygen into atomic oxygen. The emission spectroscopy of the plasma plume reveals that the plasma effluent, which carries abundant atomic oxygen, extends from the cap of the plasma spray by about 25 to 30 mm. Tests on blood droplets and smeared blood samples revealed the effectiveness and mechanism of low temperature air plasma on clotting blood. Tests on oral pathogens show that air plasma creates a zone of microbial growth inhibition in each of six treated samples, including those of grampositive bacteria and fungi, and on a cultivating biofilm sample of Streptococcus mutans UA159. The medical applications of the air plasma sprays for 1) bleeding control, 2) wound healing, and 3) dental disinfection, are then illustrated and discussed. As animal models, pigs were used in the tests of stopping wound bleeding and post-operative observation of wound healing by this air plasma spray. The results show that the bleeding from a cut to an ear artery is stopped swiftly; this air plasma spray also shortens wound healing time to about half (from 14 days to 8 days) after stopping the bleeding of a cross cut wound in the ham area. In-vitro tests demonstrate that the plasma effluent of the spray can prevent the formation of dental biofilms and further eliminate the mature biofilms.

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The authors declare no conflicts of interest.

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Kuo, S. (2012) Air plasma for medical applications. Journal of Biomedical Science and Engineering, 5, 481-495. doi: 10.4236/jbise.2012.59061.


[1] Laroussi, M. (1996) Sterilization of contaminated matter with an atmosphere pressure plasma. IEEE Transactions on Plasma Science, 24, 1188-1191. doi:10.1109/27.533129
[2] Herrmann, H.W., Henins, I., Park, J. and Selwyn, G.S. (1999) Decontamination of chemical and biological warfare (CBW) agents using an atmospheric pressure plasma jet (APPJ). Physics of Plasmas, 6, 2284-2289. doi:10.1063/1.873480
[3] Lai, W., Lai, H., Kuo, S. P., Tarasenko, O. and Levon, K. (2005) Decontamination of biological warfare agents by a microwave plasma torch. Physics of Plasmas, 12, 023501- 023506. doi:10.1063/1.1843131
[4] Baxter, H.C., Campbell, G.A., Whittaker, A.G., Aitken, A., Simpson, A.H., Casey, M., Jones, A.C., Bountiff, L., Gibbard, L. and Baxter, R.L. (2005) Elimination of TSE infectivity and decontamination of surgical instruments using RF gas-plasma treatment. Journal of General Virology, 86, 2393-2399. doi:10.1099/vir.0.81016-0
[5] Tarasenko, O., Nourkbash, S., Kuo, S.P., Bakhtina, A., Alusta, P., Kudasheva, D., Cowman, M. and Levon, K. (2006) Scanning electron and atomic force microscopy to study plasma torch effects on B. cereus spores. IEEE Transactions on Plasma Science, 34, 1281-1289. doi:10.1109/TPS.2006.878378
[6] Kuo, S.P., Tarasenko, O., Nourkbash, S., Bakhtina, A. and Levon, K. (2006) Plasma effects on bacterial spores in a wet environment. New Journal of Physics, 8, 41 (1- 11).
[7] Kalghatgi, S.U., Fridman, G., Cooper, M., Nagaraj, G., Peddinghaus, M., Balasubramanian, M., Vasilets, V.N., Gutsol, A.F., Fridman, A. and Friedman, G. (2007) Mechanism of blood coagulation by nonthermal atmospheric pressure dielectric barrier discharge plasma. IEEE Transactions on Plasma Science, 35, 1559-1566. doi:10.1109/TPS.2007.905953
[8] Kuo, S.P., Tarasenko, O., Popovic, S. and Levon, K. (2006) Killing of bacterial spores contained in a paper envelope by a microwave plasma torch. IEEE Transactions on Plasma Science, 34, 1275-1280.
[9] Kuo, S.P. (2006) Portable Arcseeded Microwave Plasma Torch. US Patent No. 7091441 B1.
[10] Kuo, S.P., Popovic, S., Tarasenko, O., Rubinraut, M. and Raskovic, M. (2007) Fanshaped microwave plasma for mail decontamination. Plasma Sources Science & Technology, 16, 581-586.
[11] Kuo, S.P. (2007) Mail Decontaminator. China Patent No. I 288005.
[12] Kuo, S., Tarasenko, O., Chang, J., Popovic, S., Chen, C., Fan, H., Scott, A., Lahiani, M., Alusta, P., Drake, J. and Nikolic, M. (2009) Contribution of a portable air plasma torch to rapid blood coagulation as a method of preventing bleeding. New Journal of Physics, 11, 115016 (1-17).
[13] Ambrosio, G., Oriente, A., Napoli, C., Palumbo, G., Chiariello, P., Marone, G., Condorelli, M., Chiariello, M. and Triggiani, M. (1994) Oxygen radicals inhibit human plasma acetylhydrolase, the enzyme that catabolizes platelet-activating factor. Journal of Clinical Investigation, 93. 2408-2416. doi:10.1172/JCI117248
[14] Kalghatgi, S.U., Fridman, G., Cooper, M., Nagaraj, G., Peddinghaus, M., Balasubramanian, M., Vasilets, V.N., Gutsol, A.F., Fridman, A. and Friedman, G. (2007) Mechanism of blood coagulation by nonthermal atmospheric pressure dielectric barrier discharge plasma. IEEE Transactions on Plasma Science, 35, 1559-1566. doi:10.1109/TPS.2007.905953
[15] Fridman, G., Peddinghaus, M., Ayan, H., Fridman, A., Balasubramanian, M., Gutsol, A., Brooks, A.D. and Friedman, G. (2006) Blood coagulation and living tissue sterilization by floating electrode dielectric barrier discharge in air. Plasma Chemistry and Plasma Processing, 26, 425-442. doi:10.1007/s11090-006-9024-4
[16] Chen, C.Y., Fan, H.W., Kuo, S.P., Chang, J., Pedersen, T., Mills, T. and Huang, C.C. (2009) Blood clotting by low temperature air plasma. IEEE Transactions on Plasma Science, 37, 993-999. doi:10.1109/TPS.2009.2016344
[17] Jevon P. and Cooper, L. (2005) First aid. Part 5. First-aid treatment for severe bleeding. Nursing Times, 104, 26-27.
[18] Spinella, P.C., Perkins, J.G., McLaughlin, D.F., Niles, S.E., Grathwohl, K.W., Beekley, A.C., Salinas, J., Mehta, S., Wade, C.E. and Holcomb, J.B. (2008) The effect of recombinant activated factor VII on mortality in combat-related casualties with severe trauma and massive transfusion. Journal of Trauma, 64, 286-294. doi:10.1097/TA.0b013e318162759f
[19] Kramer, A.H., Gurka, M.J., Nathan, B., Dumont, A.S., Kassell, N.F. and Bleck, T.P. (2008) Complications associated with anemia and blood transfusion in patients with aneurysmal subarachnoid hemorrhage. Critical Care Medicine, 36, 2070-2075. doi:10.1097/CCM.0b013e31817c1095
[20] Perkins, J.G., Cap, A.P., Weiss, B.M., Reid, T.J. and Bolan, C.D. (2008) Massive transfusion and nonsurgical hemostatic agents. Critical Care Medicine, 36, S325-S339. doi:10.1097/CCM.0b013e31817e2ec5
[21] Scharf, R.E. (2008) Acquired platelet function disorders: pathogenesis, classification, frequency, diagnosis, clinical management. Hamostaseologie, 28, 299-311.
[22] Trouillas, P. and von Kummer, R. (2006) Classification and athogenesis of cerebral hemorrhages after thrombolysis in ischemic stroke. Stroke, 37, 556-561. doi:10.1161/01.STR.0000196942.84707.71
[23] Franchini, M. (2008) Surgical prophylaxis in von Willebrand’s disease: A difficult balance to manage. Blood Transfusion, 6, S33-S38.
[24] Al-Sallami, H., Ferguson, R., Wilkins, G., Gray, A. and Medlicott, N.J. (2008) Bleeding events in patients receiving enoxaparin for the management of non-ST-elevation acute coronary syndrome (NSTEACS) at Dunedin Public Hospital, New Zealand. New Zealand Medical Journal, 121, 87-95.
[25] Roy, P., Bonello, L., Torguson, R., de Labriolle, A., Lemesle, G., Slottow, T., Steinberg, D., Kaneshige, K., Xue, Z., Satler, L., Kent, K., Suddath, W., Pichard, A., Lindsay, J. and Waksman, R. (2008) Impact of “nuisance” bleeding on clopidogrel compliance in patients undergoing intracoronary drug-eluting stent implantation. American Journal of Cardiology, 102, 1614-1617. doi:10.1016/j.amjcard.2008.07.063
[26] Milovanov, A.P. and Kirsanov, Ia.N. (2008) The pathogenesis of uterine hemorrhages in the so-called placental polyps. Arkhiv Patologii Journal, 70, 34-37.
[27] Jamal, M.M., Samarasena, J.B. and Hashemzadeh, M. (2008) Decreasing in-hospital mortality for oesophageal variceal hemorrhage in the USA. European Journal of Gastroenterology & Hepatology, 20, 947-955. doi:10.1097/MEG.0b013e32830280c7
[28] Thanvi, B.R., Treadwell, S. and Robinson, T. (2008) Haemorrhagic transformation in acute ischaemic stroke following thrombolysis therapy: classification, pathogenesis and risk factors. Postgraduate Medical Journal, 84, 361-367. doi:10.1136/pgmj.2007.067058
[29] USA Today (2009) Advanced first aid for troops sought. 14 September 2009, A1.
[30] Petersen, P.E., Bourgeois, D., Ogawa, H., Estupinan-Day, S. and Ndiaye, C. (2005) The global burden of oral diseases and risks to oral health. WHO Bulletin, 83, 661-669.
[31] Beighton, D. (2005) The complex oral microflora of high-risk individuals and groups and its role in the caries process. Community Dentistry and Oral Epidemiology, 33, 248-255. doi:10.1111/j.1600-0528.2005.00232.x
[32] Costerton, J.W., Stewart, P.S. and Greenberg, E.P. (1999) Bacterial biofilms: A common cause of persistent infections. Science, 284, 1318-1322. doi:10.1126/science.284.5418.1318
[33] Schilling, K.M. and Bowen, W.H. (1992) Glucans synthesized in situ in experimental salivary pellicle function as specific binding sites for Streptococcus mutans. Infection and Immunity, 60, 284-295.
[34] Kuo, S.P. (2010) Portable Plasma Sterilizer. US Patent No. 7777151.
[35] Kuo, S.P., Pedersen, T. and Mills, T. (2008) Lateral distribution of atomic oxygen flux produced by an array of three fanshaped plasma torches. IEEE Transactions on Plasma Science, 36, 1056-1057. doi:10.1109/TPS.2004.924556
[36] Kuo, S.P., Pedersen, T. and Mills, T. (2011) Two-dimensional distribution of atomic oxygen multiplet radiation Produced by an air plasma torch. IEEE Transactions on Plasma Science, 39, 2282-2283. doi:10.1109/TPS.2011.2155089
[37] Georg, A., Engemann, J. and Brockhaus, A. (2002) Investigation of a pulsed oxygen microwave plasma by time-resolved two-photon allowed laser-induced fluorescence. Journal of Physics D, 35, 875-881. doi:10.1088/0022-3727/35/9/307
[38] Henderson, W.R., Fite, W.L. and Brackmann, R.T. (1969) Dissociative attachment of electrons to hot oxygen. Physical Review, 183, 157-166. doi:10.1103/PhysRev.183.157
[39] Anthea, M., et al. (1993) Human biology and health. Prentice Hall, Englewood Cliffs.
[40] Reimers, R.C., Sutera, S.P. and Joist, H.J. (1984) Potentiation by red blood cells of shear-induced platelet aggregation: Relative importance of chemical and physical mechanisms. Blood, 64, 1200-1206.
[41] Sachs, U.J. and Nieswandt, B. (2007) In vivo thrombus formation in murine models. Circulation Research, 100, 979-991. doi:10.1161/01.RES.0000261936.85776.5f
[42] Kolev, K., Longstaff, C. and Machovich, R. (2005) Fibrinolysis at the fluid-solid interface of thrombi. Current Medicinal Chemistry—Cardiovascular & Hematological Agents, 3, 341-355. doi:10.2174/156801605774322337
[43] Bethesda, L.D. (2005) Blood Groups and Red Cell Antigens. National Library of Medicine (US): NCBI.
[45] Greer, J.P., et al. (2003) Wintrobe’s clinical hematology. 11th Edition, Lippincott Williams & Wilkins, Philadelphia.
[46] Fridman, G., Shereshevsky, A., Jost, M., Brooks, A., Fridman, A., Gutsol, A., Vasilets, V. and Friedman, G. (2007) Floating electrode dielectric barrier discharge plasma in air promoting apoptotic behavior in melanoma skin cancer cell lines. Plasma Chemistry & Plasma Processing, 27, 163-176. doi:10.1007/s11090-007-9048-4
[47] Machovich, R. and Owen, W.G. (1990) The elastase-mediated pathway of fibrinolysis. Blood Coagulation & Fibrinolysis, 1, 79-90. doi:10.1097/00001721-199003000-00011
[48] Born, G.V.R., Bergquist, D. and Arfors, K.E. (1976) Evidence for inhibition of platelet activation in blood by a drug effect on erythrocytes. Nature, 259, 233-235. doi:10.1038/259233a0
[49] Bergquist, D. and Arfors, K.E. (1980) Haemostatic platelet plug formation in the isolated rabbit mesenteric preparation—An analysis of red blood cell participation. Thrombosis and Haemostasis, 44, 6-8.
[50] Schmid-Schdnbein, H., et al. (1979) Basic aspects of blood trauma. Schattauer Verlag, Stuttgart, 322-340.
[51] Tiefenbach, H. J., Durchschlag, H., Schneider, G. and Jaenicke, R. (2004) Thermodynamic analysis of serum albumin denaturation by sodium dodecyl sulfate. Aqueous polymer dispersions, 124, 130-140.
[52] Schmid-Schdnbein, H., Born, G.V.R., Richardson, P.D., Cusack, N., Rieger, H., Forst, R., Rohling-Winkel, J., Blasberg, P. and Wehmeyer, A. (1981) Rheology of thrombotic processes in flow: The interaction of erythrocytes and thrombocytes subjected to high flow forces. Biorheology, 18, 415-444.
[53] Meireles, M., Aimar, P. and Sanchez, V. (2004) Albumin denaturation during ultrafiltration: Effects of operating conditions and consequences on membrane fouling. Biotechnology and Bioengineering, 38, 528-534. doi:10.1002/bit.260380511
[54] Ricciardi, M.J., Knight, B.P., Martinez, F.J. and Ruben-fire, M. (1998) Inhaled nitric oxide in primary hypertension: A safe and effective agent for predicting response to nifedipine. Journal of the American College of Cardiology, 32, 1068-1073. doi:10.1016/S0735-1097(98)00361-1
[55] Mathisen, D.J., Kuo, E.Y., Hahn, C., et al. (1998) Inhaled nitric oxide for adult respiratory distress syndrome after pulmonary resection. The Annals of Thoracic Surgery, 66, 1894-1902. doi:10.1016/S0003-4975(98)01167-9
[56] Weber, A., Strobach, H. and Schror, K. (1993) Direct inhibition of platelet function by organic nitrates via nitric oxide formation. European Journal of Pharmacology, 247, 29-37. doi:10.1016/0922-4106(93)90134-U
[57] Moro, M.A., Russell, R.J., Cellek, S., et al. (1996) GMP mediates the vascular and platelet actions of nitric oxide: Confirmation using an inhibitor of the soluble guanylyl cyclase. Proceedings of the National Academy of Sciences, 93, 1480-1485. doi:10.1073/pnas.93.4.1480
[58] Aoki, H., Inoue, M., Mizobe, T., et al. (1997) Platelet function is inhibited by nitric oxide liberation during nitroglycerin-induced anaesthesia. British Journal of Anaesthesia, 79, 476-481. doi:10.1093/bja/79.4.476
[59] Nong, Z., Hoylaerts, M., Van, Pelt N., et al. (1997) Nitric oxide inhalation inhibits platelet aggregation and plate-let-mediated pulmonary thrombosis in rats. Circulation Research, 81, 865-869. doi:10.1161/01.RES.81.5.865
[60] Brune, B. and Hanstein, K. (1998) Rapid reversibility of nitric oxide induced platelet inhibition. Thrombosis Research, 90, 83-91. doi:10.1016/S0049-3848(98)00024-3
[61] Gries, A., Bode, C., Peter, K., et al. (1998) Inhaled nitric oxide inhibits human platelet aggregation, pselectin expression, and fibrinogen binding in vitro and in vivo. Circulation, 97, 1481-1487. doi:10.1161/01.CIR.97.15.1481
[62] Koo, H., Gomes, B.P., Rosalen, P.L., Ambrosano, G.M., Park, Y.K. and Cury, J.A. (2000) In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens. Archives of Oral Biology, 45, 141-148. doi:10.1016/S0003-9969(99)00117-X
[63] Kuo, S., Chen, C.Y., Lin, C.S. and Chiang, S.H. (2010) Wound bleeding control by low temperature air plasma. IEEE Transactions on Plasma Science, 38, 1908-1914. doi:10.1109/TPS.2010.2047028
[64] Kuo, S.P., Chen, C.Y., Lin, C.S. and Chiang, S.H. (2012) Applications of air plasma for wound bleeding control and healing. IEEE Transactions on Plasma Science, 40, 1117-1123. doi:10.1109/TPS.2012.2184142
[65] Finazzi-Agro’, A., Menichelli, A., Persiani, M., Biancini, G. and Del Principe, D. (1982) Hydrogen peroxide release from human blood platelets. Biochimica et Biophysica Acta, 718, 21-25. doi:10.1016/0304-4165(82)90004-6
[66] Del Principe, D., Menichelli, A., De Matteis, W., Di Giulio, S., Giordani, M., Savini, I. and Finazzi-Agro’, A. (1991) Hydrogen peroxide is an intermediate in the platelet activation cascade triggered by collagen, but not by thrombin. Thrombosis Research, 62, 365-375. doi:10.1016/0049-3848(91)90010-T
[67] Del Principe, D., Menichelli, A., De Matteis, W., Di Corpo, M.L., Di Giulio, S. and Finazzi-Agro’, A. (1985) Hydrogen peroxide has a role in the aggregation of human platelets. FEBS Letters, 185, 142-146. doi:10.1016/0014-5793(85)80758-4
[68] Pratico, D., Iuliano, L., Alessandri, C., Camastra, C. and Violi, F. (1993) Polymorphonuclear leukocyte-derived O2- reactive species activate primed platelets in human whole blood. American Journal of Physiology, 264, H1582-H1587.
[69] Madison, K.C. (2003) Barrier function of the skin: “La raison d'être” of the epidermis. Journal of Investigative Dermatology, 121, 231-241. doi:10.1046/j.1523-1747.2003.12359.x
[70] Nguyen, D.T. Orgill, D.P. and Murphy, G.F. (2009) Biomaterials for treating skin loss. CRC Press, Boca Ratons.
[71] Clark, R.A.F. (1996) Wound repair: Overview and general considerations. In: The molecular and cellular boilogy of wound repair. Plenum Press, New York, 3-50.
[72] Levenson, S.M., Geever, E.F., Crowley, L.V., Oates, J.F., Berard, C.W. and Rosen, H. (1965) The healing of rat skin wounds. Annals of Surgery, 161, 293-308.
[73] Davis, J.C. and Hunt, T.K. (1988) Problem wounds: The role of oxygen. Elsevier, Berlin.
[74] La Van, F.B. and Hunt, T.K. (1990) Oxygen and wound healing. Clinics in Plastic Surgery, 17, 463-472.
[75] Duarte, S., Gregoire, S., Singh, A.P., Vorsa, N., Schaich, K., Bowen, W.H. and Koo, H. (2006) Inhibitory effects of cranberry polyphenols on formation and acidogenicity of Streptococcus mutans biofilms. FEMS Microbiology Letters, 257, 50-56. doi:10.1111/j.1574-6968.2006.00147.x
[76] Cegelski, L., Marshall, G.R., Eldridge, G.R. and Hultgren, S.J. (2008) The biology and future prospects of antivirulence therapies. Nature Reviews Microbiology, 6, 17-27. doi:10.1038/nrmicro1818
[77] Duarte, S., Kuo, S.P., Murata, R.M., Chen, C.Y., Saxena, D., Huang, K.J. and Popovic, S. (2011) Air plasma effect on dental disinfection. Physics of Plasmas, 18, 073503 (1-7).

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