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A β-Glucosidase Activity Potentially Involved in Cell Division and Wall Development of Phyllostachys Bamboo Suspension Cells

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DOI: 10.4236/ajps.2012.38127    3,169 Downloads   5,434 Views   Citations

ABSTRACT

We propose a novel Madake (Phyllostachys bambusoides) bamboo suspension culture model for investigation of key enzyme(s) activity involved in growth/differentiation. Sedimented Cell Volume (SCV) and fresh weight (FW) of the suspension cultured cells reached 34% (v/v) and 8.7 g in 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D)-containing medium while only 7% (v/v) SCV and 1.9 g FW of the cells in 10 μM gibberellic acid (GA3)-containing medium in 14 days. Proportion of mitotically active cells (S to G2/M phases) at a log phase was identified as 29.5% in the former cells with tiny cytoplasmic features while 5.4% in the latter cells with elongation, wall thickening, and lignification by using flow cytometry and laser scanning microscopic analysis. The total β-glucosidase (BGL) activity under the 2,4-D condition increased from 4.8 U in day 2 to 26.2 U in day 14 (ca. 5.5-fold) while a slight reduction, from 4.4 U in day 2 to 2.1 U in day 14 (ca. 0.5-fold), occurred when cell division was suppressed under the GA3 condition. Ratio of the BGL activity of the soluble fractions to the membrane-associated fractions varied depending of the culture condition. The ratio was stable (2 to 8) during the culture period under the 2,4-D condition. Interestingly, the activity of the soluble enzyme fractions increased up to ca. 65% under the GA3 condition in inverse proportion to the membrane-associated enzymes. All together, it was strongly suggested that the detected specificity/variability of BGL activity is potentially involved in cell division and lignification in Madake bamboo cells.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

S. Ogita, S. Ohki, T. Nomura and Y. Kato, "A β-Glucosidase Activity Potentially Involved in Cell Division and Wall Development of Phyllostachys Bamboo Suspension Cells," American Journal of Plant Sciences, Vol. 3 No. 8, 2012, pp. 1066-1072. doi: 10.4236/ajps.2012.38127.

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