Share This Article:

The Use of Exoskeletons of Shrimp (Litopenaeus vanammei) and Crab (Ucides cordatus) for the Extraction of Chitosan and Production of Nanomembrane

Abstract Full-Text HTML Download Download as PDF (Size:3594KB) PP. 495-508
DOI: 10.4236/msa.2012.37070    6,438 Downloads   10,106 Views   Citations


Chitin is widely distributed in nature, being the main structural component of the exoskeleton of crustaceans and is non-toxic, biodegradable and biocompatible. These exoskeletons once discarded become an industrial waste creating environmental pollutant. In order to find an alternative use, the present work exploits the extraction of the chitosan from chitin that is present in the exoskeletons of shrimps Litopenaeus vannamei and crabs Ucides cordatus and transforms it into high valued products, which can help solving the environmental problem as well to provide extra income to the fishermen. One example is the manufacture of nanomembranes from chitosan for the application in medical textiles. Nanomembranes using electrospinning of chitosan solutions (7% and 5wt%) with 100:0 v/v (TFA/DCM) and 70:30 v/v (TFA/DCM) were produced. Morphological properties of chitin and chitosan were studied using SEM, DRX, and thermal properties through TG/DTG and molecular structure by FTIR analysis. TG/DTG showed thermal decomposition of chitosan samples. X-ray diffraction analysis indicated the semi-crystalline structure of chitosan, and highly crystalline structure for chitin. Morphologies of the nanomembranes were also observed from scanning electron micrographs. Results showed that the nanomembranes with 5% chitosan solutions with 70:30 v/v (TFA/DCM) showed facilitation in the formation of the nanomembranes. The nanomembranes of shrimp and crab with 5% 70:30 v/v (TFA/DCM) had higher breaking tension and breaking extension. With positive results obtained, the present work will help the authorities to organize the fishermen to have consciousness in the collection of exoskeleton waste as well as helping to have a better environment.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

S. Andrade, R. Ladchumananandasivam, B. Rocha, D. Belarmino and A. Galvão, "The Use of Exoskeletons of Shrimp (Litopenaeus vanammei) and Crab (Ucides cordatus) for the Extraction of Chitosan and Production of Nanomembrane," Materials Sciences and Applications, Vol. 3 No. 7, 2012, pp. 495-508. doi: 10.4236/msa.2012.37070.


[1] A. Gildberg and E. Stenberg, “A New Process for Ad- vanced Utilization of Shrimp Waste,” Process Biochem- istry, Vol. 36, No. 8-9, 2001, pp. 809-812. doi:10.1016/S0032-9592(00)00278-8
[2] E. Tonhi and A. M. G. Plepis, “Obten??o e caracteriza??o de blendas colágeno-quitosana,” Química Nova, Vol. 25, No. 6, 2002, pp. 943-948.
[3] K. Okuyama, K. Noguchi, M. Kanenari, T. Egawa, K. Osawa and K. Ogawa, “Structural Diversity of Chitosan and Its Complexes,” Osaka Prefecture University, Sakai, 1999.
[4] I. Y. Kimura, J. R. Gon?alves, J. Stolberg, M. C. M. Laranjeira and V. T. Favere, “Efeito do pH e do tempo de contato na adsor??o de corantes reativos por microesferas de quitosana,” Polímeros, Vol. 9, No. 3, 1999, p. 51.
[5] Y. Noishiki, Y. Nishiyama, M. Wada, S. Okada and S. Kuga, “Inclusion Complex of Beta-Chitin and Aliphatic Amines,” Biomacromolecules, Vol. 4, No. 4, 2003, pp. 944-949. doi:10.1021/bm034024k
[6] F. S. P. Campana, D. Britto, E. Curti, F. R. Abreu, M. B. Cardoso, M. V. Battisti, P. C. Sim, R. C. Goy, R. Signini, R. L. Lavall, “Extra??o, estruturas e propriedades de a- e b-quitina,” Química Nova, Vol. 30, No. 3, 2007.
[7] F. C. Wu, R. L. Tseng and R. S. Juang, “Enhanced Abili- ties of Highly Swollen Chitosan Beads for Colour Re- moval and Tyrosinase Immobilization,” Journal of Haz- ardous Materials, Vol. 81, No. 1-2, 2001, pp. 167-177. doi:10.1016/S0304-3894(00)00340-X
[8] M. Rinaudo, “Chitin and Chitosan Properties and Appli- cations,” Progress in Polymer Science, Vol. 31, No. 7, 2006, pp. 603-632.doi:10.1016/j.progpolymsci.2006.06.001
[9] P. Abram, “Chitin e Chitosan: Obtención caracterización y aplicaciones,” Pontificia Universidad Católica-Perú, 2004.
[10] R. M. Madri, “Camar?es-Como está o agro negócio camar?o marinho,” Revista Brasileira de Agropecuária, Vol. 11, 2001, p. 66.
[11] ABCC-Associa??o Brasileira de Criadores de Camar?o.
[12] G. A. S. Melo, “Manual de identifica??o dos Brachyura (caranguejos e siris) do litoral brasileiro,” Editora Plêiade, S?o Paulo, 1996.
[13] G. G. Castilho, “Aspectos reprodutivos do caranguejo- u?á,Ucides cordatus (Crustacea,Brachyura, Ocypodidae), Baía de Antonina, Paraná, Brasil. Curitiba.” Disserta??o (Mestrado em Ciências Veterinária)—Setor de Ciências Agrárias, Universidade Federal do Paraná, 2006.
[14] M. A. A. Pinheiro and A. G. Fiscarelli, “Manual de apoio à fiscaliza??o do caranguejo-u?á (Ucides cordatus),” Santa Catarina, Centro de Pesquisa e Gest?o de Recursos Pesqueiros do Litoral Sudeste e Su—CEPSUL, Itajaí, 2001.
[15] A. A. Craveiro, A. C. Craveiro and D. C. Queiroz, “Qui- tosana: A fibra do futuro,” Editora Universitária, Pernam- buco, 1998.
[16] I. P. Rocha, J. Rodrigues and L. A. Amorim, “Carcini- cultura brasileira em 2003,” Revista da Associa??o Brasileira de Criadores de Camar?o-30, 2004.
[17] N. M. Soares, C. M. Moura, J. Rizzi, S. R. Vasconcelos, V. O. B. Santos and L. A. A. Pinto, “Study of the Chito- san Production Starting from Shrimp Residues in Pilot Scale,” XVI CRICTE_Regional Congress of Scientific and Technological Initiation in Engineering, Ijuí, 2003.
[18] J. M. Moura, F. C. Ferreira, F. M. M. Silva, J. Rizzi and L. A. A. Pinto, “Obten??o de quitina a partir de carapa?as de siri—Uso de um Planejamento Experimental na Etapa de Desmineraliza??o,” Vol. 15, 2005, pp. 7-17.
[19] G. A. F. Roberts, “Chitin Chemistry,” The Macmillan Press Ltd., Hong Kong, 1992.
[20] N. A. R. Gow, G. W. Goodway, J. D. Russell and M. J. Wilson, “Infrared and X-Ray Diffraction Data on Chitins of Variable Structure,” Carbohydrate Research, Vol. 165, 1987, pp. 105-110.
[21] J. Brugnerotto, J. Lizardi, F. M. Goycoolea, W. Arguel- las-Monal, J. Desbrières and M. Rinaudo, “An Infrared Investigation in Relation with Chitin and Chitosan Char- acterization”, Polymer, Vol. 42, No. 8, 2001, pp. 35-69. doi:10.1016/S0032-3861(00)00713-8

comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.