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Influence of Bavistin and Silver Thiosulphate on in Vitro Regeneration of Asclepias curassavica (L). Using Nodal Explants

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DOI: 10.4236/ajps.2012.37111    4,414 Downloads   8,082 Views   Citations

ABSTRACT

The effect of bavistin and the ethylene inhibitor (silver thiosulphate) on shoot regeneration using nodal explants of Asclepias curassavica (L). has been investigated. Among the different concentrations studied, highest number of shoots was obtained on MS media with 200 mg/L bavistin. Among the varying concentrations (10-100 μM/L) of silver thio-sulphate tested, highest number of shoots was obtained on MS medium amended with 60 μM/L silver thiosulphate without growth regulators. This study also establishes the stronger cytokinin like activity of bavistin. Effect of different growth additives like coconut milk, ascorbic acid and casein hydrolysate were tested on direct shoot regeneration. Among the different growth additives tested casein hydrolysate showed better and reproducible result at 0.025% in combination with 3 mg/l KN + 0.5 mg/l NAA. Antioxidants, activated charcoals and polyvinyl pyrrolidone were used to remove phenolics. Activated charcoal removed the phenolic exudates completely at 0.025% and prevented the browning of media and thus enhanced the frequency of regeneration (85%). The microshoots developed through in vitro regeneration were transferred to rooting media containing IBA alone and in combination with KN and the highest number of roots was observed on MS medium with IBA 1 mg/L + 0.2 mg/L KN.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

S. Hemadri Reddy, M. Chakravarthi, K. Chandrashekara and C. Naidu, "Influence of Bavistin and Silver Thiosulphate on in Vitro Regeneration of Asclepias curassavica (L). Using Nodal Explants," American Journal of Plant Sciences, Vol. 3 No. 7, 2012, pp. 941-946. doi: 10.4236/ajps.2012.37111.

References

[1] M. E. Endress and P. V. Bruyns “A revised classification of the Apocynaceae s.l.,” Botanical Review, No. 66, 2000, pp. 1-56.
[2] S. S. Samant, U. Dhar and L. M. S. Palni “Medicinal Plants of Indian Himalaya: Diversity, Distribution Potential Values,” Himavikas Publication, No. 13, Gyan Prakash, Nainital, 1998.
[3] N. Komalavalli and M. V. Rao. “In vitro micropropagation of Gymnema elegans – a rare medicinal plant,” Indian Journal of Experimental Biology, Vol. 35, 1997, pp. 1088-1092.
[4] P. Knogstrup, S. Baldursson and J. V. Norgard “Ex situ genetic conservation by use of tissue culture,” Operational Botany, Vol. 113, 1992, pp. 49-53.
[5] M. F. Fay “In what is in vitro culture appropriate to plant conservation?,” Biodiversity and Conservation, Vol. 3, 1994, pp. 176-183.
[6] I. K. Vasil and T. A. Thorpe “Plant cell and tissue culture,” Kluwer Academic Publishers, Dordrecht, 1994.
[7] S. Govil and S. C. Gupta “Commercialisation of plant tissue culture in India,” Plant Cell, Tissue and Organ Culture, Vol. 51, 1997, pp. 65-73.
[8] R. Shields, S. J. Robinson, and P. A. Anslow “Use of fungicides in plant tissue culture,” Plant Cell Reports, Vol. 3, 1984, pp. 33-36.
[9] L. Burgos and N. Alburquerque, “Ethylene inhibitors and low kanamycin concentrations improve adventitious regeneration from apricot leaves,” Plant Cell Reports, Vol. 21, 2003, pp. 1167-1174.
[10] E. M. Beyer (1979). “Effect of silver ion, carbon dioxide, and oxygen on ethylene action and metabolism,” Plant Physiology, Vol. 63, 1979, pp. 169-173.
[11] G. L. Chi, D. G. Barfield, G. E. Sim and E. C. Pua “Effect of AgNO3 and aminoethoxyvinylglycine on in vitro shoot and root organogenesis from seedling explants of recalcitrant Brassica genotypes,” Plant Cell Reports, Vol. 9, 1990, pp. 195-198.
[12] D. D. Songstad, D. R. Duncan and J. M. Widholm “Effect of 1-aminocyclopropane-1-carboxylic acid, silver nitrate, and noborn-adiene on plant regeneration from maize callus cultures,” Plant Cell Reports, Vol. 7, 1988, pp. 262-265.
[13] K. M. Charaibi, A. Latche, J. P. Roustan, and J. Fallot “Stimulation of shoot regeneration from cotyledons of Helianthus annuus by the ethylene inhibitors, silver and cobalt,” Plant Cell Reports, Vol. 10, 1991, pp.204-207.
[14] H. P. Bais, G. Sudha and G. A. Ravishankar “Influence of putrescine, silver nitrate and polyamine inhibitors on the morphogenetic response in untransformed and transformed tissues of Cichorium intybus and their regenerants,” Plant Cell Reports, Vol. 20, 2001, pp. 547-555.
[15] P. Sujana and C. V. Naidu “Influence of Bavistin, Cefotaxime, Kanamycin and Silver Thiosulphate on Plant Regeneration of Mentha piperita (L.) – An Important Multi-purpose Medicinal Plant,” Journal of Phytology, Vol. 3, 2011, pp. 36-40.
[16] D. Preethi, T. M. Sridhar and C. V. Naidu “Effect of Bavistin and Silver Thiosulphate on In Vitro Plant Regeneration of Stevia rebaudiana,” Journal of Phytology, Vol. 3, 2011, pp. 74-77.
[17] P. Bantawa, O. S. Roy, P. Ghosh and T. K. Mondal “Effect of Bavistin and Adenine Sulphate on In vitro Shoot Multiplication of Picrorhiza scrophulariiflora Pennell.: An Endangered Medicinal Plant of Indo‐China Himalayan Regions,” Plant Tissue Culture and Biotechnology, Vol. 19, 2009, pp.237-245.
[18] N. Komalavalli and M. V. Rao “In vitro micropropagation of Gymnema sylvestre-a multipurpose medicinal plant,” Plant Cell, Tissue and Organ Culture, Vol. 61, 2000, pp. 97-105.
[19] C. J. Delp “Benzimidazole and related fungicides,” In: Modern selective fungicides: properties, applications, mechanisms of action, Wiley, New York, 1987.
[20] R. K. Tripathi and S. Ram “Induction of growth and differentiation of carrot callus cultures by carbendazim and benzimidazole,” Indian Journal of Experimental Biology, Vol. 20, 1982, pp. 674-677.
[21] P. C. Garcia, R. M. Rivero, J. M. Ruiz and L. Romero “The role of fungicides in the physiology of higher plants: implications for defense responses,” Botanical Review, Vol. 69, 2003, pp. 162-172.
[22] K. G. M. Skene, K.G.M. “Cytokinin-like properties of the systemic fungicide benomyl,” Journal of Horticultural Science, Vol. 47, 1972, pp. 179-182.
[23] T. H. Thomas “Investigations into the cytoki-nin-like properties of benzimidazole derived fungicides” Annals of Applied Biology, Vol. 76, 1974, pp. 237-241.
[24] D. Raghuramulu “In vitro morphogenetic studies of Hemidesmus indicus and Cynanchum callialatum. (Asclepiadaceae),” Ph.D. Thesis, Sri Krishnadevaraya University, Ananthapur, India, 2001.
[25] R. W. Joy-Iv, K. R. Patel and T. A. Thorpe “Ascorbic acid enhancement of organogenesis in tobacco callus,” Plant Cell, Tissue and Organ Culture, Vol. 13, 1988, pp. 219-228.
[26] A. Ahuja, M. Verma and S. Grewal “Clonal propagation of Ocomum species by Tissue Culture,” Indian Journal of Experimental Biology, Vol. 20, 1982, pp. 455-458.
[27] N. Sharma and K. P. S. Chandel “Effect of ascorbic acid on axillary shoot induction in Tylophoro indica (Burm. f.) Merril,” Plant Cell, Tissue and Organ Culture, Vol. 29, 1992b, pp. 109-113.
[28] V. Sarasan, E. Y. Sonia and G. M. Nair “Regeneration of Indian sarasapilla, Hemidesmus indicus R. Br., through organogenesis and somatic embryogenesis,” Indian Journal of Experimental Biology, Vol. 32, 1994, pp. 284-287.
[29] E. F. George and P. D. Sherrington “Handbook and directory of commercial laboratories,” In: plant propagation by tissue culture, Exegeties Limited, Eversley. U.K, 1984.
[30] C. G. Sudha “Tissue culture studies on three rare medicinal plants of India, Holostemma anulare, Janakia arayalpathra and Rauwolfia micrantha”. Ph. D. Thesis, University of Kerala, India, 1996.
[31] T. Chakradhar “In vitro culture, physiological, phytochemical and antimicrobial studies of a Medicinal plant. Wattakaka volubilis (L.f.) stapf (Asclepiadaceae),” Ph.D. thesis submitted to S.K. University, Ananthapur, India, 2004.

  
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