Share This Article:

Protective Effect of Aloe vera (Aloe barbadensis Miller) on Erythrocytes Anion Transporter and Oxidative Change

Abstract Full-Text HTML XML Download Download as PDF (Size:589KB) PP. 1697-1702
DOI: 10.4236/fns.2012.312222    4,855 Downloads   7,980 Views   Citations


The purpose of this study was to evaluate the ability of aqueous extract of Aloe barbadensis Miller (Aloe vera) on oxidative damage and Anion Exchanger 1 (AE1, also known as Band 3) expression in human erythrocytes exposed to the water soluble free radical initiator 2.2’-azobis-2-amidinopropano dihydrochloride (AAPH). In addition, total phenolic compounds in the extracts were determined as catechin equivalent and the various antioxidant activities were compared to natural and synthetic standard antioxidants such as BHA and ascorbic acid. Since Aloe vera extract did not cause a consumption of the cytosolic antioxidant, glutathione (GSH) when it was direct incubated with GSH in basic aerated aqueous solution, this indicates that Aloe vera extract does not proceed auto oxidation at this experimental condition. Furthermore, Aloe vera extract prevent the consumption of GSH, in radical treated RBCs. It also inhibit consumption of GSH when it was direct incubated with AAPH. Aloe vera gel extract inhibits the generation of diphenyl-2-picrylhy-drazyl (DPPH) and the scavenging activity was increased in a dose dependent manner. Aloe vera extract was shown the similar reducing power than standards BHT and ascorbic acid. Biochemical analysis by SDS-PAGE and western blotting showed that AAPH-induced oxidative stress increased the susceptibility of AE1 to proteolytic degradation. Of note, our data evidenced that Aloe vera treatment was able to partially restore the normal RBC membrane protein profiles in a dose-dependent manner. These results clearly demonstrate the antioxidative activity of Aloe vera gel extract that might be ascribed to a synergistic action of the bioactive compounds contained therein.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

S. Mazzulla, S. Sesti, A. Schella, I. Perrotta, A. Anile and S. Drogo, "Protective Effect of Aloe vera (Aloe barbadensis Miller) on Erythrocytes Anion Transporter and Oxidative Change," Food and Nutrition Sciences, Vol. 3 No. 12, 2012, pp. 1697-1702. doi: 10.4236/fns.2012.312222.


[1] U. Eggli, “Illustrated Handbook of Succulent plants: Monocotyledons,” Springer, New York, 2001. doi:10.1007/978-3-642-56715-5
[2] M. D. Boudreau and F. A. Beland, “An Evaluation of the Biological and Toxicological Properties of Aloe Barbadensis (Miller), Aloe Vera,” Journal of Environmental Science and Health Part C Environmental Carcinogenesis & Ecotoxicology Reviews, Vol. 24, No 1, 2006, pp. 103-154. doi:10.1080/10590500600614303
[3] P. Rishi, A. Rampuria, R. Tewari and A. Koul, “Phytomodulatory Potentials of Aloe Vera against Salmonella OmpR-Mediated Inflammation,” Phytotherapy Research, Vol. 22, No. 8, 2008, pp.1075-1082. doi/10.1002/ptr.2458/abstract
[4] O. A. Wintola and A. J. Afolayan, “Phytochemical Constituents and Antioxidant Activities of the Whole Leaf Extract of Aloe Ferox Mill,” Pharmacognosy Magazine, Vol. 7, No. 28, 2011, pp. 325-333. doi:10.4103/0973-1296.90414
[5] F. Habeeb, E. Shakir, F. Bradbury, P. Cameron, M. R. Taravati, A. J. Drummond, A. I. Gray and V. A. Ferro, “Screening Methods Used to Determine the Anti-Microbial Properties of Aloe vera Inner Gel,” Methods, Vol. 42, No. 4, 2007, pp. 315-320. doi:10.1016/j.ymeth.2007.03.004
[6] K. Kim, H. Kim, J. Kwon, S. Lee, H. Kong, S.-A. Im, Y.-H. Lee, Y.-R. Lee, S.-T. Oh, T. H. Jo, Y. l. Park and C.-K. Lee, “Hypoglycemic and Hypolipidemic Effects of Processed Aloe vera Gel in a Mouse Model of Non-In- sulin-Dependent Diabetes Mellitus,” Phytomedicine, Vol. 16, No. 9, 2009, pp. 856-863.
[7] V. Steenkamp and M. J. Stewart, “Medicinal Applications and Toxicological Activities of Aloe Products,” Pharmaceutical Biology, Vol. 45, No. 5, 2007, pp. 411-420. doi:10.1080/13880200701215307
[8] J. H. Hamman, “Composition and Applications of Aloe vera Leaf Gel,” Molecules, Vol. 13, No 8, 2008, pp. 1599-1616.
[9] M. A. Gbadegesin, O. A. Odunola, K. A. Akinwumi and O. O. Osifeso, “Comparative Hepatotoxicity and Clastogenicity of Sodium Arsenite and Three Petroleum Products in Experimental Swiss Albino Mice: The Modulatory Effects of Aloe vera Gel,” Food and Chemical Toxicology, Vol. 47, No. 10, 2009, pp. 2454-2457. doi:10.1016/j.fct.2009.07.002
[10] N. Yun, C. H. Lee and S. M. Lee, “Protective Effect of Aloe vera on Polymicrobial Sepsis in Mice,” Food and Chemical Toxicology, Vol. 47, No. 6, 2009, pp. 1341- 1348. doi:10.1016/j.fct.2009.03.013
[11] M. J. A. Tanner, “The Structure and Function of Band 3 (AE1): Recent Developments (Review),” Molecular Membrane Biology, Vol.14, No. 4, 1997, pp. 155-165. doi:10.3109/09687689709048178
[12] L. J. Bruce and M. J. A. Tanner, “Erythroid Band 3 Variants and Disease,” Baillière’s Clin Haematol, Vol. 12, No. 4, 1999, pp. 637-654. doi:10.1053/beha.1999.0046
[13] T. Somer and H. J. Meiselman, “Disorders of Blood Viscosity,” Annals of Medicine, Vol. 25, No. 1, 1993, pp. 31-39. doi:10.3109/07853899309147854
[14] K. Slinkard and V. L. Singleton, “Total Phenol Analyses: Automation and Comparison with Manual Methods,” American Journal of Enology and Viticulture, Vol. 28, No. 1, 1977, pp. 49-55.
[15] W. Brand-Williams, M. E. Cuvelier and C. Berset, “Use of a Free Radical Method to Evaluate Antioxidant Activity,” LWT—Food Science and Technology, Vol. 28, No. 1, 1995, pp. 25-30. doi:10.1016/S0023-6438(95)80008-5
[16] M. Oyaizu, “Studies on Product of Browning Reaction Prepared from Glucose Amine,” Japanese Journal of Nutrition, Vol. 44, No. 6, 1986, pp. 307-315. doi:10.5264/eiyogakuzashi.44.307
[17] F. N. Ko, G. Hsiao and Y. H. Kuo “Protection of Oxidative Hemolysis by Demethyldiisoeugenol in Normal and Beta-Thalassemic Red Blood Cells,” Free Radical Biology & Medicine, Vol. 22, No. 1-2, 1997, pp. 215-222.
[18] E. Beutler, O. Duron and B. M. Kelly, “Improved Method for the Determination of Blood Glutathione,” Journal of Laboratory and Clinical Medicine, Vol. 61, No. 3, 1963, pp. 882-888.
[19] J. T. Dodge, C. Mitchell and D. J. Hanahan, “The Preparation and Chemical Characteristics of Hemoglobin-Free Ghosts of Human Erythrocytes,” Archives of Biochemistry and Biophysics, Vol. 100, No. 1, 1963, pp. 119-130. doi:10.1016/0003-9861(63)90042-0
[20] M. M. Bradford, “A Rapid and Sensitive Method for the Quantification of Microgram Quantities of Protein Utilizing the Principle of the Protein Dye Binding,” Analytical Biochemistry, Vol. 72, No. 1-2, 1976, pp. 248-254. doi:10.1016/0003-2697(76)90527-3
[21] M. Skerget, P. Kotnik, M. Hadolin, A. R. Hras, M. Simonic and Z. Knez, “Phenols, Proanthocyanidins, Flavones and Flavonols in Some Plant Materials and Their Antioxidant Activities,” Food Chemistry, Vol. 89, No. 2, 2005, pp. 191-198. doi:10.1016/j.foodchem.2004.02.025
[22] V. Saritha, K. R. Anilakumar and F. Khanum, “Antioxidant and Antibacterial Activity of Aloe vera Gel Extracts,” International Journal of Pharmaceutical & Biological Archive, Vol. 1, No 4, 2010, pp. 376-384.
[23] G. Williamson and C. Manach, “Bioavailability and Bioefficacy of Polyphenols in Humans. II. Review of 93 Intervention Studies,” The American Journal of Clinical Nutrition, Vol. 81, No. 1, 2005, pp. 243S-255S.
[24] M. Miki, H. Tamai, M. Mino, Y. Yamamoto and E. Niki, “Free Radical Chain Oxidation of Rat Red Blood Cells by Molecular Oxygen and Its Inhibition by A-Tocopherol,” Archives of Biochemistry and Biophysics, Vol. 258, No. 2, 1987, pp. 373-380. doi:10.1016/0003-9861
[25] L. L. Peters, R. A. Shivdasani, S.-C. Liu, M. Hanspal, K. M. John, J. M. Gonzalez, C. Brugnara, B. Gwynn, N. Mohandas, S. L. Alper, S. H. Orkin and S. E. Lux, “Anion Exchanger 1 (Band 3) Is Required to Prevent Erythrocyte Membrane Surface Loss but Not to Form the Membrane Skeleton,” Cell, Vol. 86, No. 6, 1996, PP. 917-927. doi:10.1016/S0092-8674(00)80167-1
[26] G. J. C. G. M. Bosman, M. Stappers and V. M. J. Novotny, “Changes in Band 3 Structure as Determinants of Erythrocyte Integrity during Storage and Survival after Transfusion,” Blood Transfusion, Vol. 8, No. 3, 2010, pp. s48-s52. doi:10.2450/2010.008S

comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.