ss="ls3">-dihydoxy flavones CR M Naringenine CRM
X between-bottle (s1,%) 99.31 ± 0.018 99.58 ± 0.013 99.58 ± 0.015
within-bottle (s2,%) 99.31 ± 0.011 99.58 ± 0.010 99.58 ± 0.010
F-test
22
12
/Fss=
2.63 1.86 2.60
0.05
F (14,6)
3.96 3.96 3.96
0.05
FF<
Y Y Y
t-test
12
txx= −
0.0010% 0.0011% 0.0022%
1
22
2
12
α
12
ss
t2 nn

 

= +
 
 

 

0.0064% 0.0049% 0.0053%
tt
α
Y Y Y
Table 4. The results of stability of flavonoids CRMs.
Time interval Baicalein 5,7-dihydoxy fl avones Naringenine
Zero month 99.27 99.50 99.53
One month 99.37 99.56 99.57
Three months 99.31 99.54 99.58
Six months 99.31 99.52 99.50
Twelve months 99.35 99.46 99.55
Average (%) 99.32 99.52 99.55
RSD (%) 0.04 0.04 0.03
Table 5. The results of uncertainty evaluation of flavonoids CRMs.
Uncertainty Baicalein CRM 5,7-dihydoxy flavones CRM Naringenine CRM
u1/% 0.01 0.02 0.03
u2/% 0.04 0.04 0.01
u3/% 0.36 0.33 0.50
u4/% 0 0 0
u5/% 0.06 0.06 0.06
u6/% 0.01 0.01 0.01
uc/% 0.37 0.34 0.50
k 2 2 2
U/% 0.8 0.7 1.0
Purity value/% 98.8 99.1 99.5
REFERENCES
[1] Arunachalama, J., Bleiseb, A., Mahwar, R.S., et al. (2006)
The natural matrix reference material database of the In-
ternational Atomic Energy Agency: Reference materials
in support of dietary investigations. Journal of Food
Composition and Analysis, 19, 241-249.
http://dx.doi.org/10.1016/j.jfca.2005.07.002
[2] Sander, L.C., Sharpless, K.E. and Wise, S.A. (2006) Die-
tary supplement standard referenc e materials. Life S c ien c es,
78, 2044-2048.
http://dx.doi.org/10.1016/j.lfs.2005.12.037
[3] Chen, C.H., Huang, T.S., Wong, C.H. , et al. (2009) Syn-
ergistic anti-cancer effect of baicalein and silymarin on
human hepatoma HepG2 Cells. Food and Chemical Tox-
icology, 47, 638-644.
http://dx.doi.org/10.1016/j.fct.2008.12.024
[4] Middleton J r., E. and Kanda swami, C. (1992) Effects of
flavonoids on immune and inflammatory cell functions.
Biochemical Pharmacology, 43, 1167-1179.
http://dx.doi.org/10.1016/0006-2952(92)90489-6
[5] Heim, K.E., Tagliaferro, A.R. and Bobilya, D.J. (2002)
Flavonoid antioxidants: Chemistry, metabolism and struc-
ture-activity relationships. Journal of Nutritional Bio-
chemistry, 13, 572-584.
http://dx.doi.org/10.1016/S0955-2863(02)00208-5
[6] The Directorate for the Quality of Medicines of the
Council of Europe (2007) Establishment of reference
standards, in European Pharmacopoeia. 5th Edition, Sup-
plement 5.6, General texts 5.12.4, Council of Europe,
Strasbourg.
[7] World Health Organization (2003) Evaluation of chemi-
C. Quan et al. / Agricultural Sciences 4 (2013) 89-96
Copyright © 2013 SciRes. OPEN A CCESS
96
cal reference substance. In: International Pharmacopoeia,
3rd Editon, Vol . 5, Supplementary Information, Part A,
3.3, World Health Organization, Geneva.
[8] Liu, S.Y. and Hu, C.Q. (2007) A comparative uncertainty
study of the calibration of macrolide antibiotic reference
standards using quantitative nuclear magnetic resonance
and mass balance methods. Analytica Chimica Acta, 602,
114-121. http://dx.doi.org/10.1016/j.aca.2007.09.009
[9] JJG1006-1994 (1994) Technical norm of primary refer-
ence material of china. Beijing.
[10] Quan, H. (2002) Reference material and its application
technology. China Standard Press, Beijing. (in Chinese)
[11] Dai, X.H., Fang, X., Shao, M.W., Li, M., et al. (2011)
Certified reference materials (GBW09170 and 09171) of
creatinine in human serum. Journal of Chromatography B,
879, 429-435.
http://dx.doi.org/10.1016/j.jchromb.2010.12.031
[12] EURACHEM/CITAC Guide CG4 (2000) Quantifying
uncertainty in analytical measurement. Seconded.
[13] Li, Y.X., Suo, Q.L., He, W.Z., et al. (2008) Separation
and Purification and Structural Characterization of Bai-
calein. Spectroscopy and Spectral Analysis, 28, 1895-
1899.
[14] NIST, NIST Chemistry WebBook.
http://webbook.nist.gov/
[15] Wang, Y.G., Xie, S.W. and Su, W.W. (2005) St udy on the
chemical constituents of Polygonum chinese L. Journal of
Chinese Medicinal Materials, 28, 1000-1001.
[16] Quan, C., Su, F.H., Wang, H.F., et al. (2011) Develop-
ment of anabolic-androgenic steroids purity certified ref-
erence materials for anti-doping. Steroids, 76, 1527-1534.
http://dx.doi.org/10.1016/j.steroids.2011.09.001